Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant

Graeme Iain Paton, B. J. Reid, K. T. Semple

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > beta CD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.

Original languageEnglish
Pages (from-to)1643-1648
Number of pages6
JournalEnvironmental Pollution
Volume157
Issue number5
DOIs
Publication statusPublished - May 2009

Keywords

  • bioluminescent bacteria
  • organic contaminants
  • biodegradation
  • extraction
  • bioavailability

Cite this

Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant. / Paton, Graeme Iain; Reid, B. J.; Semple, K. T.

In: Environmental Pollution, Vol. 157, No. 5, 05.2009, p. 1643-1648.

Research output: Contribution to journalArticle

@article{28ad5b51ea014a2b99f83046ce38a14a,
title = "Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant",
abstract = "Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > beta CD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.",
keywords = "bioluminescent bacteria, organic contaminants, biodegradation, extraction, bioavailability",
author = "Paton, {Graeme Iain} and Reid, {B. J.} and Semple, {K. T.}",
year = "2009",
month = "5",
doi = "10.1016/j.envpol.2008.12.020",
language = "English",
volume = "157",
pages = "1643--1648",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "ELSEVIER APPL SCI PUBL LTD",
number = "5",

}

TY - JOUR

T1 - Application of a luminescence-based biosensor for assessing naphthalene biodegradation in soils from a manufactured gas plant

AU - Paton, Graeme Iain

AU - Reid, B. J.

AU - Semple, K. T.

PY - 2009/5

Y1 - 2009/5

N2 - Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > beta CD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.

AB - Despite numerous reviews suggesting that microbial biosensors could be used in many environmental applications, in reality they have failed to be used for which they were designed. In part this is because most of these sensors perform in an aqueous phase and a buffered medium, which is in contrast to the nature of genuine environmental systems. In this study, a range of non-exhaustive extraction techniques (NEETs) were assessed for (i) compatibility with a naphthalene responsive biosensor and (ii) correlation with naphthalene biodegradation. The NEETs removed a portion of the total soil naphthalene in the order of methanol > HPCD > beta CD > water. To place the biosensor performance to NEETs in context, a biodegradation experiment was carried out using historically contaminated soils. By coupling the HPCD extraction with the biosensor, it was possible to assess the fraction of the naphthalene capable of undergoing microbial degradation in soil. Exposure of microbial biosensors to cyclodextrin solutions allows the assessment of the degradable fraction of contaminants in soil.

KW - bioluminescent bacteria

KW - organic contaminants

KW - biodegradation

KW - extraction

KW - bioavailability

U2 - 10.1016/j.envpol.2008.12.020

DO - 10.1016/j.envpol.2008.12.020

M3 - Article

VL - 157

SP - 1643

EP - 1648

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

IS - 5

ER -