Application of elemental bioimaging using laser ablation ICP-MS in forest pathology: distribution of elements in the bark of Picea sitchensis following wounding

Magdalena Siebold, Patrick Leidich, Martina Bertini, Giuliana Deflorio, Joerg Feldmann, Eva M. Krupp, Erhard Halmschlager, Steve Woodward

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Element distribution in the bark of two 20-year-old clones of Picea sitchensis following wounding was studied using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Bark was sampled at 0, 3, and 43 days after wounding and analysed using a focused Nd:YAG laser (266 nm). Intensities of (13) C, Mg-25, Al-27, P-31, S-32, K-39, Ca-48, Mn-55, Fe-57, Cu-63 and Zn-64 were measured by ICP-MS to study elemental distribution across the bark samples during the wound repair process. A clear accumulation of Mg, P and K at the boundary zone between the lesion and healthy tissue was detected in the wounded samples and was more distinctive at 43 than at 3 days after treatment. This zone of accumulation mapped onto the position of formation of the ligno-suberised boundary zone and differentiation of the wound periderm. These accumulations suggest major roles for Mg, P and K in the non-specific response of Sitka spruce both to wounding, possibly as co-factors to enzymes and energy utilisation. The LA-ICP-MS method developed in this work proved useful to study spatial element distribution across bark samples and has great potential for applications in other areas of plant pathology research.

Original languageEnglish
Pages (from-to)3323-3331
Number of pages9
JournalAnalytical and Bioanalytical Chemistry
Volume402
Issue number10
Early online date15 Jan 2012
DOIs
Publication statusPublished - Apr 2012

Keywords

  • Bark
  • Element distribution
  • Element mapping
  • Element bio-imaging
  • Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS)
  • Sitka spruce
  • Plasma-mass-spectrometry
  • Heterobasidion-annosum
  • Sitka Spruce
  • Trace-elements
  • Norway
  • Abies
  • Wood
  • Susceptibility
  • Stilbenes
  • Pathogens

Cite this

Application of elemental bioimaging using laser ablation ICP-MS in forest pathology : distribution of elements in the bark of Picea sitchensis following wounding. / Siebold, Magdalena; Leidich, Patrick; Bertini, Martina; Deflorio, Giuliana; Feldmann, Joerg; Krupp, Eva M.; Halmschlager, Erhard; Woodward, Steve.

In: Analytical and Bioanalytical Chemistry, Vol. 402, No. 10, 04.2012, p. 3323-3331.

Research output: Contribution to journalArticle

Siebold, Magdalena ; Leidich, Patrick ; Bertini, Martina ; Deflorio, Giuliana ; Feldmann, Joerg ; Krupp, Eva M. ; Halmschlager, Erhard ; Woodward, Steve. / Application of elemental bioimaging using laser ablation ICP-MS in forest pathology : distribution of elements in the bark of Picea sitchensis following wounding. In: Analytical and Bioanalytical Chemistry. 2012 ; Vol. 402, No. 10. pp. 3323-3331.
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abstract = "Element distribution in the bark of two 20-year-old clones of Picea sitchensis following wounding was studied using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Bark was sampled at 0, 3, and 43 days after wounding and analysed using a focused Nd:YAG laser (266 nm). Intensities of (13) C, Mg-25, Al-27, P-31, S-32, K-39, Ca-48, Mn-55, Fe-57, Cu-63 and Zn-64 were measured by ICP-MS to study elemental distribution across the bark samples during the wound repair process. A clear accumulation of Mg, P and K at the boundary zone between the lesion and healthy tissue was detected in the wounded samples and was more distinctive at 43 than at 3 days after treatment. This zone of accumulation mapped onto the position of formation of the ligno-suberised boundary zone and differentiation of the wound periderm. These accumulations suggest major roles for Mg, P and K in the non-specific response of Sitka spruce both to wounding, possibly as co-factors to enzymes and energy utilisation. The LA-ICP-MS method developed in this work proved useful to study spatial element distribution across bark samples and has great potential for applications in other areas of plant pathology research.",
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AB - Element distribution in the bark of two 20-year-old clones of Picea sitchensis following wounding was studied using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). Bark was sampled at 0, 3, and 43 days after wounding and analysed using a focused Nd:YAG laser (266 nm). Intensities of (13) C, Mg-25, Al-27, P-31, S-32, K-39, Ca-48, Mn-55, Fe-57, Cu-63 and Zn-64 were measured by ICP-MS to study elemental distribution across the bark samples during the wound repair process. A clear accumulation of Mg, P and K at the boundary zone between the lesion and healthy tissue was detected in the wounded samples and was more distinctive at 43 than at 3 days after treatment. This zone of accumulation mapped onto the position of formation of the ligno-suberised boundary zone and differentiation of the wound periderm. These accumulations suggest major roles for Mg, P and K in the non-specific response of Sitka spruce both to wounding, possibly as co-factors to enzymes and energy utilisation. The LA-ICP-MS method developed in this work proved useful to study spatial element distribution across bark samples and has great potential for applications in other areas of plant pathology research.

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