Application of luminescent biosensors for monitoring the degradation and toxicity of BTEX compounds in soils

J. J. C. Dawson, C. O. Iroegbu, H. Maciel, G. I. Paton

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Aims: To assess the changes in acute toxicity and biodegradation of benzene, toluene, ethylbenzene and xylene (collectively referred to as BTEX) compounds in soil over time and compare the performances of biological and chemical techniques.

Methods and Results: Biological methods (lux-based bacterial biosensors, basal respiration and dehydrogenase activity) were related to changes in the concentration of the target compounds. There was an initial increase in toxicity determined by the constitutively expressed biosensor, followed by a continual reduction as degradation proceeded. The biosensor with the BTEX-specific promoter was most induced when BTEX concentrations were highest. The treatment with nutrient amendment had a significant increase in microbial activity, while the sterile control produced the lowest level of degradation.

Significance and Impact of the Study: Luminescent biosensors were able to monitor changes in contaminant toxicity and bioavailability in aqueous extracts from BTEX-impacted soils as degradation proceeded. The integration of biological tests with chemical analysis enables a fuller understanding of the biodegradation processes occurring at their relative rates.

Conclusions: The biological methods were successfully used in assessing the performance of different treatments for enhancing natural attenuation of BTEX from contaminated soils. While, chemical analysis showed biodegradation of parent BTEX compounds in biologically active soils, the biosensor assays reported on changes in bioavailability and potentially toxic intermediate fractions as they estimated the integrative effect of contaminants.

Original languageEnglish
Pages (from-to)141-151
Number of pages11
JournalJournal of Applied Microbiology
Volume104
Issue number1
Early online date8 Oct 2007
DOIs
Publication statusPublished - Jan 2008

Keywords

  • biodegradation
  • BTEX
  • luminescent biosensors
  • remediation
  • soil
  • lux-modified bacterial
  • contaminated soil
  • hydrocarbon biodegradation
  • microbial-degradation
  • long-term
  • bioremediation
  • indicators
  • recovery
  • pollutants

Cite this

Application of luminescent biosensors for monitoring the degradation and toxicity of BTEX compounds in soils. / Dawson, J. J. C.; Iroegbu, C. O.; Maciel, H.; Paton, G. I.

In: Journal of Applied Microbiology, Vol. 104, No. 1, 01.2008, p. 141-151.

Research output: Contribution to journalArticle

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