Abstract
There are significant concerns about the impact of heavy metal contamination in soils as a consequence of urbanisation and industrialisation in developing countries. Routine chemical analysis of soils is used to measure the total concentration of metals from point source or diffuse activities, but this fails to put in context the bioavailability of the analyte or the potential toxicity of multiple contaminants. Bacterial biosensors provide a useful tool for assessing the toxicity of the bioavailable fraction of heavy metals in soils and for complementing chemical analysis. There are few examples of genuine environmental applications of biosensors for pollutant diagnosis. This study applied constitutively marked biosensors (which were comprehensively characterised) to soils collected from across Northern China (60,000 km 2). The biosensors were responsive to soils impacted by As, Cd, Cr, Cu, Hg, Pb, and Zn when compared to 'uncontaminated controls'. The response of the biosensor correlated with individual (or groups of) metals related to their concentration and source. The geo-accumulation index (I geo) assisted in explaining the biosensor response. The constitutively marked biosensors offered a focussed understanding of analyte bioavailability and placed in a relevant context the elemental analysis. When matrix-matched control samples can be collected, then such a biosensor procedure (as adopted here) is applicable to contrasting soils exposed to a wide range of contaminants. Biosensor applications complemented routine soil chemical analysis for this regional-scale study.
| Original language | English |
|---|---|
| Article number | 1409 |
| Pages (from-to) | 1-16 |
| Number of pages | 16 |
| Journal | Water, Air and Soil Pollution |
| Volume | 224 |
| Issue number | 2 |
| Early online date | 8 Jan 2013 |
| DOIs | |
| Publication status | Published - Feb 2013 |
Fingerprint
Keywords
- Bacterial biosensors
- Bioavailability
- Heavy metal contamination
- Soil
ASJC Scopus subject areas
- Pollution
- Environmental Chemistry
- Environmental Engineering
- Ecological Modelling
- Water Science and Technology
Cite this
Application of Microbial Biosensors to Complement Geochemical Characterisation: a Case Study in Northern China. / Zhang, Bo; Qiao, Min; Liu, Yunxia; Zheng, Yuanming; Zhu, Yongguan; Paton, Graeme I.
In: Water, Air and Soil Pollution, Vol. 224, No. 2, 1409, 02.2013, p. 1-16.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Application of Microbial Biosensors to Complement Geochemical Characterisation:
T2 - a Case Study in Northern China
AU - Zhang, Bo
AU - Qiao, Min
AU - Liu, Yunxia
AU - Zheng, Yuanming
AU - Zhu, Yongguan
AU - Paton, Graeme I.
N1 - This work was funded by the Chinese Academy of Sciences (KZCX2-YW-Q02-06) and the Ministry of Science and Technology, China (2007CB407301).
PY - 2013/2
Y1 - 2013/2
N2 - There are significant concerns about the impact of heavy metal contamination in soils as a consequence of urbanisation and industrialisation in developing countries. Routine chemical analysis of soils is used to measure the total concentration of metals from point source or diffuse activities, but this fails to put in context the bioavailability of the analyte or the potential toxicity of multiple contaminants. Bacterial biosensors provide a useful tool for assessing the toxicity of the bioavailable fraction of heavy metals in soils and for complementing chemical analysis. There are few examples of genuine environmental applications of biosensors for pollutant diagnosis. This study applied constitutively marked biosensors (which were comprehensively characterised) to soils collected from across Northern China (60,000 km 2). The biosensors were responsive to soils impacted by As, Cd, Cr, Cu, Hg, Pb, and Zn when compared to 'uncontaminated controls'. The response of the biosensor correlated with individual (or groups of) metals related to their concentration and source. The geo-accumulation index (I geo) assisted in explaining the biosensor response. The constitutively marked biosensors offered a focussed understanding of analyte bioavailability and placed in a relevant context the elemental analysis. When matrix-matched control samples can be collected, then such a biosensor procedure (as adopted here) is applicable to contrasting soils exposed to a wide range of contaminants. Biosensor applications complemented routine soil chemical analysis for this regional-scale study.
AB - There are significant concerns about the impact of heavy metal contamination in soils as a consequence of urbanisation and industrialisation in developing countries. Routine chemical analysis of soils is used to measure the total concentration of metals from point source or diffuse activities, but this fails to put in context the bioavailability of the analyte or the potential toxicity of multiple contaminants. Bacterial biosensors provide a useful tool for assessing the toxicity of the bioavailable fraction of heavy metals in soils and for complementing chemical analysis. There are few examples of genuine environmental applications of biosensors for pollutant diagnosis. This study applied constitutively marked biosensors (which were comprehensively characterised) to soils collected from across Northern China (60,000 km 2). The biosensors were responsive to soils impacted by As, Cd, Cr, Cu, Hg, Pb, and Zn when compared to 'uncontaminated controls'. The response of the biosensor correlated with individual (or groups of) metals related to their concentration and source. The geo-accumulation index (I geo) assisted in explaining the biosensor response. The constitutively marked biosensors offered a focussed understanding of analyte bioavailability and placed in a relevant context the elemental analysis. When matrix-matched control samples can be collected, then such a biosensor procedure (as adopted here) is applicable to contrasting soils exposed to a wide range of contaminants. Biosensor applications complemented routine soil chemical analysis for this regional-scale study.
KW - Bacterial biosensors
KW - Bioavailability
KW - Heavy metal contamination
KW - Soil
UR - http://www.scopus.com/inward/record.url?scp=84871796288&partnerID=8YFLogxK
U2 - 10.1007/s11270-012-1409-9
DO - 10.1007/s11270-012-1409-9
M3 - Article
AN - SCOPUS:84871796288
VL - 224
SP - 1
EP - 16
JO - Water, Air and Soil Pollution
JF - Water, Air and Soil Pollution
SN - 0049-6979
IS - 2
M1 - 1409
ER -