Application of response surface methodology to optimize decolourization of dyes by the laccase-mediator system

Dalel Daâssi, Fakher Frikha, Hela Zouari-Mechichi, Lassaad Belbahri, Steve Woodward, Tahar Mechichi

Research output: Contribution to journalArticle

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Abstract

Response surface methodology (RSM) was applied to optimize the decolourization of 3 dyes belonging to 3 dye families such as reactive black 5 (diazoic), indigo carmine (indigoid) and aniline blue (anthraquinonic). Crude laccase from Trametes trogii and the laccase-mediator 1-hydroxybenzotriazole (HBT) were used in this study. Box–Behnken design using RSM with six variables namely pH, temperature, enzyme concentration, HBT concentration, dye concentration and incubation time was used in this study to optimize significant correlation between the effects of these variables on the decolourization of reactive black 5 (RB5), indigo carmine (IC) and aniline blue (AB).

The optimum of pH, temperature, laccase, HBT, RB5 and reaction time were 4.5, 0.5 U ml-1, 0.5 mM, 100 mg ml-1 and 150 min respectively, for a maximum decolourization of RB5 (about 92.92% ± 7.21). Whereas, the optimum decolourization conditions of both IC (99.76% ± 7.75) and AB (98.44% ± 10) were: pH 4.5, temperature of 45 °C, enzyme concentration of 0.1 U ml-1 and 0.5 U ml-1, HBT concentration of 0.9 mM and 0.5 mM, dye concentration of 60 mg l-1 and reaction time of 150 and 90 min, respectively.

The experimental values were in good agreement with the predicted ones and the models were highly significant, the correlation coefficient (R2) being 0.864, 0.663 and 0.776 for RB5, IC and AB, respectively. In addition, when the kinetic parameters for the three dyes decolourization were calculated according to Hannes–Wolf plot, the following values were obtained: Km of 268.4, 47.94 and 44.64 mg l-1 then Vmax of 35.58, 10.43 and 9.23 mg l-1 min for the RB5, IC and AB decolourizations by laccase, respectively.
Original languageEnglish
Pages (from-to)84-91
Number of pages8
JournalJournal of Environmental Management
Volume108
Early online date1 Jun 2012
DOIs
Publication statusPublished - 15 Oct 2012

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Aniline
dye
Dyes
Enzymes
enzyme
temperature
Kinetic parameters
Temperature
incubation
response surface methodology
kinetics

Keywords

  • optimization
  • laccase
  • synthetic dyes
  • box-behnken
  • decolourization

Cite this

Application of response surface methodology to optimize decolourization of dyes by the laccase-mediator system. / Daâssi, Dalel; Frikha, Fakher; Zouari-Mechichi, Hela; Belbahri, Lassaad; Woodward, Steve; Mechichi, Tahar.

In: Journal of Environmental Management, Vol. 108, 15.10.2012, p. 84-91.

Research output: Contribution to journalArticle

Daâssi, Dalel ; Frikha, Fakher ; Zouari-Mechichi, Hela ; Belbahri, Lassaad ; Woodward, Steve ; Mechichi, Tahar. / Application of response surface methodology to optimize decolourization of dyes by the laccase-mediator system. In: Journal of Environmental Management. 2012 ; Vol. 108. pp. 84-91.
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AU - Daâssi, Dalel

AU - Frikha, Fakher

AU - Zouari-Mechichi, Hela

AU - Belbahri, Lassaad

AU - Woodward, Steve

AU - Mechichi, Tahar

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N2 - Response surface methodology (RSM) was applied to optimize the decolourization of 3 dyes belonging to 3 dye families such as reactive black 5 (diazoic), indigo carmine (indigoid) and aniline blue (anthraquinonic). Crude laccase from Trametes trogii and the laccase-mediator 1-hydroxybenzotriazole (HBT) were used in this study. Box–Behnken design using RSM with six variables namely pH, temperature, enzyme concentration, HBT concentration, dye concentration and incubation time was used in this study to optimize significant correlation between the effects of these variables on the decolourization of reactive black 5 (RB5), indigo carmine (IC) and aniline blue (AB). The optimum of pH, temperature, laccase, HBT, RB5 and reaction time were 4.5, 0.5 U ml-1, 0.5 mM, 100 mg ml-1 and 150 min respectively, for a maximum decolourization of RB5 (about 92.92% ± 7.21). Whereas, the optimum decolourization conditions of both IC (99.76% ± 7.75) and AB (98.44% ± 10) were: pH 4.5, temperature of 45 °C, enzyme concentration of 0.1 U ml-1 and 0.5 U ml-1, HBT concentration of 0.9 mM and 0.5 mM, dye concentration of 60 mg l-1 and reaction time of 150 and 90 min, respectively. The experimental values were in good agreement with the predicted ones and the models were highly significant, the correlation coefficient (R2) being 0.864, 0.663 and 0.776 for RB5, IC and AB, respectively. In addition, when the kinetic parameters for the three dyes decolourization were calculated according to Hannes–Wolf plot, the following values were obtained: Km of 268.4, 47.94 and 44.64 mg l-1 then Vmax of 35.58, 10.43 and 9.23 mg l-1 min for the RB5, IC and AB decolourizations by laccase, respectively.

AB - Response surface methodology (RSM) was applied to optimize the decolourization of 3 dyes belonging to 3 dye families such as reactive black 5 (diazoic), indigo carmine (indigoid) and aniline blue (anthraquinonic). Crude laccase from Trametes trogii and the laccase-mediator 1-hydroxybenzotriazole (HBT) were used in this study. Box–Behnken design using RSM with six variables namely pH, temperature, enzyme concentration, HBT concentration, dye concentration and incubation time was used in this study to optimize significant correlation between the effects of these variables on the decolourization of reactive black 5 (RB5), indigo carmine (IC) and aniline blue (AB). The optimum of pH, temperature, laccase, HBT, RB5 and reaction time were 4.5, 0.5 U ml-1, 0.5 mM, 100 mg ml-1 and 150 min respectively, for a maximum decolourization of RB5 (about 92.92% ± 7.21). Whereas, the optimum decolourization conditions of both IC (99.76% ± 7.75) and AB (98.44% ± 10) were: pH 4.5, temperature of 45 °C, enzyme concentration of 0.1 U ml-1 and 0.5 U ml-1, HBT concentration of 0.9 mM and 0.5 mM, dye concentration of 60 mg l-1 and reaction time of 150 and 90 min, respectively. The experimental values were in good agreement with the predicted ones and the models were highly significant, the correlation coefficient (R2) being 0.864, 0.663 and 0.776 for RB5, IC and AB, respectively. In addition, when the kinetic parameters for the three dyes decolourization were calculated according to Hannes–Wolf plot, the following values were obtained: Km of 268.4, 47.94 and 44.64 mg l-1 then Vmax of 35.58, 10.43 and 9.23 mg l-1 min for the RB5, IC and AB decolourizations by laccase, respectively.

KW - optimization

KW - laccase

KW - synthetic dyes

KW - box-behnken

KW - decolourization

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