Application of the Dynamic Gastrointestinal Simulator (simgi®) to assess the impact of probiotic supplementation in the metabolism of grape polyphenols

Irene Gil-Sánchez, Carolina Cueva, Alba Tamargo, Jose C. Quintela, Esther de la Fuente, Alan W. Walker, M. Victoria Moreno-Arribas, Begoña Bartolomé* (Corresponding Author)

*Corresponding author for this work

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In this paper, the Dynamic Gastrointestinal Simulator (simgi®) is used as a model to the study the metabolic activity of probiotics at the intestinal level, and in particular, to assess the impact of probiotic supplementation in the microbial metabolism of grape polyphenols. Two independent simulations using fecal samples from two healthy volunteers were carried out. Changes in microbiota composition and in metabolic activity were assessed by qPCR and 16S rRNA gene sequencing and by analyses of phenolic metabolites and ammonium ions (NH4+). The strain Lactobacillus plantarum CLC 17 was successfully implanted in the colon compartments of the simgi® after daily feeding of 2x1010 CFU/day for 7 days. Overall, no changes in bacterial diversity were observed after probiotic implantation. In comparison to the digestion of the grape polyphenols on their own, the inclusion of L. plantarum CLC 17 in the simgi® colon compartments led to a greater formation of phenolic metabolites such as benzoic acids, probably by the breakdown of high-molecular-weight procyanidin polymers. These results provide evidence that the probiotic strain Lactobacillus plantarum CLC 17 may improve the metabolism of dietary polyphenols when used as a food ingredient.
Original languageEnglish
Article number108790
JournalFood Research International
Volume129
Early online date21 Nov 2019
DOIs
Publication statusPublished - Mar 2020

Keywords

  • simgi® model
  • CLC 17
  • phenolic metabolites
  • metabolism
  • gut microbiota
  • Gut microbiota
  • Simgi® model
  • Phenolic metabolites
  • Lactobacillus plantarum CLC 17
  • Metabolism
  • SURVIVAL
  • PHENOLIC METABOLITES
  • HUMAN GUT MICROBIOTA
  • FECAL MICROBIOTA
  • IN-VITRO FERMENTATION
  • SMALL-INTESTINE
  • HUMAN FECES
  • LACTIC-ACID BACTERIA
  • RED WINE
  • Simgi (R) model
  • LACTOBACILLUS-ACIDOPHILUS

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