Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells

Asim K Dutta-Roy; J  Taylor; Margaret Jane Gordon; Nigel Hoggard; Fiona Margaret Campbell

doi:10.1016/S0006-291X(02)02647-5

Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells

Asim K Dutta-Roy, J Taylor, Margaret Jane Gordon, Nigel Hoggard, Fiona Margaret Campbell

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.

Original language	English
Pages (from-to)	432-437
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	299
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(02)02647-5
Publication status	Published - 6 Dec 2002

Keywords

RECEPTOR MESSENGER-RNA
FATTY-ACIDS
OBESE GENE
BINDING PROTEIN
DIABETIC MICE
OB-R
METABOLISM
EXPRESSION
LOCALIZATION
INTERNALIZATION
receptor messenger-RNA
fatty-acids
obese gene
bindingf protein
diabetic mice
metabolism
expression
localization
internalization

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10.1016/S0006-291X(02)02647-5

Cite this

@article{09f452f8697f4429b1c898fb6bb50c29,

title = "Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells",

abstract = "Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.",

keywords = "RECEPTOR MESSENGER-RNA, FATTY-ACIDS, OBESE GENE, BINDING PROTEIN, DIABETIC MICE, OB-R, METABOLISM, EXPRESSION, LOCALIZATION, INTERNALIZATION, receptor messenger-RNA, fatty-acids, obese gene, bindingf protein, diabetic mice, metabolism, expression, localization, internalization",

author = "Dutta-Roy, {Asim K} and J Taylor and Gordon, {Margaret Jane} and Nigel Hoggard and Campbell, {Fiona Margaret}",

year = "2002",

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T1 - Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells

AU - Dutta-Roy, Asim K

AU - Taylor, J

AU - Gordon, Margaret Jane

AU - Hoggard, Nigel

AU - Campbell, Fiona Margaret

PY - 2002/12/6

Y1 - 2002/12/6

N2 - Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.

AB - Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.

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KW - FATTY-ACIDS

KW - OBESE GENE

KW - BINDING PROTEIN

KW - DIABETIC MICE

KW - OB-R

KW - METABOLISM

KW - EXPRESSION

KW - LOCALIZATION

KW - INTERNALIZATION

KW - receptor messenger-RNA

KW - fatty-acids

KW - obese gene

KW - bindingf protein

KW - diabetic mice

KW - metabolism

KW - expression

KW - localization

KW - internalization

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DO - 10.1016/S0006-291X(02)02647-5

M3 - Article

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EP - 437

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

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