Abstract
Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 432-437 |
| Number of pages | 6 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 299 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 6 Dec 2002 |
Keywords
- RECEPTOR MESSENGER-RNA
- FATTY-ACIDS
- OBESE GENE
- BINDING PROTEIN
- DIABETIC MICE
- OB-R
- METABOLISM
- EXPRESSION
- LOCALIZATION
- INTERNALIZATION
- receptor messenger-RNA
- fatty-acids
- obese gene
- bindingf protein
- diabetic mice
- metabolism
- expression
- localization
- internalization
Cite this
Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells. / Dutta-Roy, Asim K; Taylor, J ; Gordon, Margaret Jane; Hoggard, Nigel; Campbell, Fiona Margaret.
In: Biochemical and Biophysical Research Communications, Vol. 299, No. 3, 06.12.2002, p. 432-437.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells
AU - Dutta-Roy, Asim K
AU - Taylor, J
AU - Gordon, Margaret Jane
AU - Hoggard, Nigel
AU - Campbell, Fiona Margaret
PY - 2002/12/6
Y1 - 2002/12/6
N2 - Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.
AB - Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.
KW - RECEPTOR MESSENGER-RNA
KW - FATTY-ACIDS
KW - OBESE GENE
KW - BINDING PROTEIN
KW - DIABETIC MICE
KW - OB-R
KW - METABOLISM
KW - EXPRESSION
KW - LOCALIZATION
KW - INTERNALIZATION
KW - receptor messenger-RNA
KW - fatty-acids
KW - obese gene
KW - bindingf protein
KW - diabetic mice
KW - metabolism
KW - expression
KW - localization
KW - internalization
U2 - 10.1016/S0006-291X(02)02647-5
DO - 10.1016/S0006-291X(02)02647-5
M3 - Article
VL - 299
SP - 432
EP - 437
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -