Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells

Asim K Dutta-Roy, J Taylor, Margaret Jane Gordon, Nigel Hoggard, Fiona Margaret Campbell

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.

Original languageEnglish
Pages (from-to)432-437
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume299
Issue number3
DOIs
Publication statusPublished - 6 Dec 2002

Keywords

  • RECEPTOR MESSENGER-RNA
  • FATTY-ACIDS
  • OBESE GENE
  • BINDING PROTEIN
  • DIABETIC MICE
  • OB-R
  • METABOLISM
  • EXPRESSION
  • LOCALIZATION
  • INTERNALIZATION
  • receptor messenger-RNA
  • fatty-acids
  • obese gene
  • bindingf protein
  • diabetic mice
  • metabolism
  • expression
  • localization
  • internalization

Cite this