Archaea predominate among ammonia-oxidizing prokaryotes in soils

S. Leininger, T. Urich, M. Schloter, L. Schwark, J. Qi, Graeme William Nicol, James Ivor Prosser, S. C. Schuster, C. Schleper

Research output: Contribution to journalArticle

1514 Citations (Scopus)

Abstract

Ammonia oxidation is the first step in nitrification, a key process in the global nitrogen cycle that results in the formation of nitrate through microbial activity(1,2). The increase in nitrate availability in soils is important for plant nutrition, but it also has considerable impact on groundwater pollution owing to leaching. Here we show that archaeal ammonia oxidizers are more abundant in soils than their well-known bacterial counterparts. We investigated the abundance of the gene encoding a subunit of the key enzyme ammonia monooxygenase (amoA) in 12 pristine and agricultural soils of three climatic zones. amoA gene copies of Crenarchaeota ( Archaea) were up to 3,000-fold more abundant than bacterial amoA genes. High amounts of crenarchaeota-specific lipids, including crenarchaeol, correlated with the abundance of archaeal amoA gene copies. Furthermore, reverse transcription quantitative PCR studies and complementary DNA analysis using novel cloning-independent pyrosequencing technology demonstrated the activity of the archaea in situ and supported the numerical dominance of archaeal over bacterial ammonia oxidizers. Our results indicate that crenarchaeota may be the most abundant ammonia-oxidizing organisms in soil ecosystems on Earth.

Original languageEnglish
Pages (from-to)806-809
Number of pages3
JournalNature
Volume442
DOIs
Publication statusPublished - Aug 2006

Keywords

  • REAL-TIME PCR
  • 16S RIBOSOMAL-RNA
  • POPULATION-SIZE
  • BACTERIA
  • CRENARCHAEOTA
  • DIVERSITY
  • LIPIDS
  • TERRESTRIAL
  • QUANTIFICATION
  • AMOA

Cite this

Leininger, S., Urich, T., Schloter, M., Schwark, L., Qi, J., Nicol, G. W., ... Schleper, C. (2006). Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature, 442, 806-809. https://doi.org/10.1038/NATURE04983

Archaea predominate among ammonia-oxidizing prokaryotes in soils. / Leininger, S.; Urich, T.; Schloter, M.; Schwark, L.; Qi, J.; Nicol, Graeme William; Prosser, James Ivor; Schuster, S. C.; Schleper, C.

In: Nature, Vol. 442, 08.2006, p. 806-809.

Research output: Contribution to journalArticle

Leininger, S, Urich, T, Schloter, M, Schwark, L, Qi, J, Nicol, GW, Prosser, JI, Schuster, SC & Schleper, C 2006, 'Archaea predominate among ammonia-oxidizing prokaryotes in soils', Nature, vol. 442, pp. 806-809. https://doi.org/10.1038/NATURE04983
Leininger S, Urich T, Schloter M, Schwark L, Qi J, Nicol GW et al. Archaea predominate among ammonia-oxidizing prokaryotes in soils. Nature. 2006 Aug;442:806-809. https://doi.org/10.1038/NATURE04983
Leininger, S. ; Urich, T. ; Schloter, M. ; Schwark, L. ; Qi, J. ; Nicol, Graeme William ; Prosser, James Ivor ; Schuster, S. C. ; Schleper, C. / Archaea predominate among ammonia-oxidizing prokaryotes in soils. In: Nature. 2006 ; Vol. 442. pp. 806-809.
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