Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle

R. John Wallace (Corresponding Author), John A. Rooke, Carol-Anne Duthie, Jimmy J. Hyslop, David W. Ross, Nest McKain, Shirley Motta de Souza, Timothy J. Snelling, Anthony Waterhouse, Rainer Roehe

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Abstract

Methane produced from 35 Aberdeen-Angus and 33 Limousin cross steers was measured in respiration chambers. Each group was split to receive either a medium- or high-concentrate diet. Ruminal digesta samples were subsequently removed to investigate correlations between methane emissions and the rumen microbial community, as measured by qPCR of 16S or 18S rRNA genes. Diet had the greatest influence on methane emissions. The high-concentrate diet resulted in lower methane emissions (P < 0.001) than the medium-concentrate diet. Methane was correlated, irrespective of breed, with the abundance of archaea (R = 0.39), bacteria (−0.47), protozoa (0.45), Bacteroidetes (−0.37) and Clostridium Cluster XIVa (−0.35). The archaea:bacteria ratio provided a stronger correlation (0.49). A similar correlation was found with digesta samples taken 2–3 weeks later at slaughter. This finding could help enable greenhouse gas emissions of large animal cohorts to be predicted from samples taken conveniently in the abattoir.
Original languageEnglish
Article number5892
Number of pages8
JournalScientific Reports
Volume4
DOIs
Publication statusPublished - 31 Jul 2014

Fingerprint

digesta
beef cattle
methane
concentrates
Archaea
diet
Limousin (cattle breed)
bacteria
Clostridium
Angus
greenhouse gas emissions
sampling
slaughterhouses
breathing
Protozoa
microbial communities
rumen
slaughter
ribosomal RNA
breeds

Keywords

  • microbiology techniques
  • genetic models
  • metabolomics
  • microbial ecology

Cite this

Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle. / Wallace, R. John (Corresponding Author); Rooke, John A.; Duthie, Carol-Anne; Hyslop, Jimmy J.; Ross, David W.; McKain, Nest; Motta de Souza, Shirley; Snelling, Timothy J.; Waterhouse, Anthony; Roehe, Rainer.

In: Scientific Reports, Vol. 4, 5892, 31.07.2014.

Research output: Contribution to journalArticle

Wallace, RJ, Rooke, JA, Duthie, C-A, Hyslop, JJ, Ross, DW, McKain, N, Motta de Souza, S, Snelling, TJ, Waterhouse, A & Roehe, R 2014, 'Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle', Scientific Reports, vol. 4, 5892. https://doi.org/10.1038/srep05892
Wallace, R. John ; Rooke, John A. ; Duthie, Carol-Anne ; Hyslop, Jimmy J. ; Ross, David W. ; McKain, Nest ; Motta de Souza, Shirley ; Snelling, Timothy J. ; Waterhouse, Anthony ; Roehe, Rainer. / Archaeal abundance in post-mortem ruminal digesta may help predict methane emissions from beef cattle. In: Scientific Reports. 2014 ; Vol. 4.
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abstract = "Methane produced from 35 Aberdeen-Angus and 33 Limousin cross steers was measured in respiration chambers. Each group was split to receive either a medium- or high-concentrate diet. Ruminal digesta samples were subsequently removed to investigate correlations between methane emissions and the rumen microbial community, as measured by qPCR of 16S or 18S rRNA genes. Diet had the greatest influence on methane emissions. The high-concentrate diet resulted in lower methane emissions (P < 0.001) than the medium-concentrate diet. Methane was correlated, irrespective of breed, with the abundance of archaea (R = 0.39), bacteria (−0.47), protozoa (0.45), Bacteroidetes (−0.37) and Clostridium Cluster XIVa (−0.35). The archaea:bacteria ratio provided a stronger correlation (0.49). A similar correlation was found with digesta samples taken 2–3 weeks later at slaughter. This finding could help enable greenhouse gas emissions of large animal cohorts to be predicted from samples taken conveniently in the abattoir.",
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