Arrest of oogenesis in the bug Rhodnius prolixus challenged with the fungus Aspergillus niger is mediated by immune response-derived PGE2

Marcelo Neves de Medeiros, Rodrigo Belmonte, Bruno César C Soares, Luciano Neves de Medeiros, Cláudio Canetti, Celio G Freire-de-Lima, Clarissa Menezes Maya-Monteiro, Patrícia Torres Bozza, Igor C Almeida, Hatisaburo Masuda, Eleonora Kurtenbach, Ednildo A Machado

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

In this work we characterized the immune response of the insect Rhodnius prolixus to a direct injection into the hemocoel of the non-entomopathogenic fungus Aspergillus niger, and evaluated its consequences on host oogenesis. These animals were able to respond by mounting effective cellular and humoral responses to this fungus; these responses were shown, however, to have reproductive fitness costs, as the number of eggs laid per female was significantly reduced. The disturbance of egg formation during infectious process correlated with an elevation in the titer of hemolymph prostaglandin E2 48 h post-challenge. Administration of Zymosan A as an immunogenic non-infectious challenge produced similar effects on phenoloxidase and prophenoloxidase activities, oocyte development and prostaglandin E2 titer, precluding the hypothesis of an effect mediated by fungal metabolites in animals challenged with fungus. Ovaries at 48 h post-challenge showed absence of vitellogenic ovarian follicles, and the in vivo administration of prostaglandin E2 or its receptor agonist misoprostol, partially reproduced this phenotype. Together these data led us to hypothesize that immune-derived prostaglandin E2 raised from the insect response to the fungal challenge is involved in disturbing follicle development, contributing to a reduction in host reproductive output and acting as a host-derived adaptive effector to infection.
Original languageEnglish
Pages (from-to)151-158
Number of pages8
JournalJournal of Insect Physiology
Volume55
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Rhodnius
Rhodnius prolixus
Oogenesis
Aspergillus niger
oogenesis
Dinoprostone
prostaglandins
Fungi
immune response
fungi
Insects
Genetic Fitness
prophenoloxidase
Misoprostol
zymosan
hemocoel
insects
Zymosan
Ovarian Follicle
Monophenol Monooxygenase

Keywords

  • adaptation, biological
  • analysis of variance
  • animals
  • Aspergillus niger
  • dinoprostone
  • female
  • mass spectrometry
  • oogenesis
  • ovary
  • rhodnius
  • zymosan

Cite this

Medeiros, M. N. D., Belmonte, R., Soares, B. C. C., Medeiros, L. N. D., Canetti, C., Freire-de-Lima, C. G., ... Machado, E. A. (2009). Arrest of oogenesis in the bug Rhodnius prolixus challenged with the fungus Aspergillus niger is mediated by immune response-derived PGE2. Journal of Insect Physiology, 55(2), 151-158. https://doi.org/10.1016/j.jinsphys.2008.10.019

Arrest of oogenesis in the bug Rhodnius prolixus challenged with the fungus Aspergillus niger is mediated by immune response-derived PGE2. / Medeiros, Marcelo Neves de; Belmonte, Rodrigo; Soares, Bruno César C; Medeiros, Luciano Neves de; Canetti, Cláudio; Freire-de-Lima, Celio G; Maya-Monteiro, Clarissa Menezes; Bozza, Patrícia Torres; Almeida, Igor C; Masuda, Hatisaburo; Kurtenbach, Eleonora; Machado, Ednildo A.

In: Journal of Insect Physiology, Vol. 55, No. 2, 02.2009, p. 151-158.

Research output: Contribution to journalArticle

Medeiros, MND, Belmonte, R, Soares, BCC, Medeiros, LND, Canetti, C, Freire-de-Lima, CG, Maya-Monteiro, CM, Bozza, PT, Almeida, IC, Masuda, H, Kurtenbach, E & Machado, EA 2009, 'Arrest of oogenesis in the bug Rhodnius prolixus challenged with the fungus Aspergillus niger is mediated by immune response-derived PGE2', Journal of Insect Physiology, vol. 55, no. 2, pp. 151-158. https://doi.org/10.1016/j.jinsphys.2008.10.019
Medeiros, Marcelo Neves de ; Belmonte, Rodrigo ; Soares, Bruno César C ; Medeiros, Luciano Neves de ; Canetti, Cláudio ; Freire-de-Lima, Celio G ; Maya-Monteiro, Clarissa Menezes ; Bozza, Patrícia Torres ; Almeida, Igor C ; Masuda, Hatisaburo ; Kurtenbach, Eleonora ; Machado, Ednildo A. / Arrest of oogenesis in the bug Rhodnius prolixus challenged with the fungus Aspergillus niger is mediated by immune response-derived PGE2. In: Journal of Insect Physiology. 2009 ; Vol. 55, No. 2. pp. 151-158.
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AU - Soares, Bruno César C

AU - Medeiros, Luciano Neves de

AU - Canetti, Cláudio

AU - Freire-de-Lima, Celio G

AU - Maya-Monteiro, Clarissa Menezes

AU - Bozza, Patrícia Torres

AU - Almeida, Igor C

AU - Masuda, Hatisaburo

AU - Kurtenbach, Eleonora

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N2 - In this work we characterized the immune response of the insect Rhodnius prolixus to a direct injection into the hemocoel of the non-entomopathogenic fungus Aspergillus niger, and evaluated its consequences on host oogenesis. These animals were able to respond by mounting effective cellular and humoral responses to this fungus; these responses were shown, however, to have reproductive fitness costs, as the number of eggs laid per female was significantly reduced. The disturbance of egg formation during infectious process correlated with an elevation in the titer of hemolymph prostaglandin E2 48 h post-challenge. Administration of Zymosan A as an immunogenic non-infectious challenge produced similar effects on phenoloxidase and prophenoloxidase activities, oocyte development and prostaglandin E2 titer, precluding the hypothesis of an effect mediated by fungal metabolites in animals challenged with fungus. Ovaries at 48 h post-challenge showed absence of vitellogenic ovarian follicles, and the in vivo administration of prostaglandin E2 or its receptor agonist misoprostol, partially reproduced this phenotype. Together these data led us to hypothesize that immune-derived prostaglandin E2 raised from the insect response to the fungal challenge is involved in disturbing follicle development, contributing to a reduction in host reproductive output and acting as a host-derived adaptive effector to infection.

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