Arsenate causes differential acute toxicity to two P-deprived genotypes of rice seedlings (Oryza sativa L.)

C N Geng, Y G Zhu, Y Hu, Paul Nicholas Williams, A A Meharg

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Significant genotypic difference in response to arsenate toxicity in rice (Oryza sativa) was investigated in root elongation, arsenate uptake kinetics, physiological and biochemical response and arsenic (As) speciation. Uptake kinetics data showed that P-deprived genotype 94D-54 had a little higher As uptake than P-deprived 94D-64, but the difference was not large enough to cause acute toxicity in P-deprived 94D-54. There was no difference in tissue P concentrations between the two genotypes under P deficient conditions. In addition, arsenic speciation in plant tissues (using high performance liquid chromatography-inductively coupled plasma mass spectrometry) was not different between P pretreatments and between genotypes. P-deprived genotype 94D-54 suffered much higher stress induced by arsenate toxicity than P-deprived genotype 94D-64, in terms of lipid peroxidation, tissue H2O2 concentrations and exosmosis of K, P and As. However, P-deprived 94D-54 also had higher overproduction of enzymatic antioxidants (with higher GPX, SOD, CAT) and NPT (non-protein thiols) than P-deprived 94D-64. It appeared that, the higher sensitivity of P-deprived 94D-54 to arsenate toxicity might cause the overproduction of NPT, thus leading to the depletion of GSH and to the accumulation of H2O2. The differential sensitivity of the two genotypes has major implications for breeding rice for As affected paddy soil.

Original languageEnglish
Pages (from-to)297-306
Number of pages10
JournalCommunications in Soil Science and Plant Analysis
Volume279
DOIs
Publication statusPublished - 2006

Keywords

  • acute toxicity
  • antioxidants
  • arsenic
  • rice
  • stress level
  • PLASMA-MASS SPECTROMETRY
  • INDUCED OXIDATIVE STRESS
  • HOLCUS-LANATUS
  • ION CHROMATOGRAPHY
  • CADMIUM TOXICITY
  • PHOSPHATE-UPTAKE
  • PLANTS
  • GLUTATHIONE
  • PHYTOCHELATINS
  • TOLERANCE

Cite this

Arsenate causes differential acute toxicity to two P-deprived genotypes of rice seedlings (Oryza sativa L.). / Geng, C N ; Zhu, Y G ; Hu, Y ; Williams, Paul Nicholas; Meharg, A A .

In: Communications in Soil Science and Plant Analysis, Vol. 279, 2006, p. 297-306.

Research output: Contribution to journalArticle

Geng, C N ; Zhu, Y G ; Hu, Y ; Williams, Paul Nicholas ; Meharg, A A . / Arsenate causes differential acute toxicity to two P-deprived genotypes of rice seedlings (Oryza sativa L.). In: Communications in Soil Science and Plant Analysis. 2006 ; Vol. 279. pp. 297-306.
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abstract = "Significant genotypic difference in response to arsenate toxicity in rice (Oryza sativa) was investigated in root elongation, arsenate uptake kinetics, physiological and biochemical response and arsenic (As) speciation. Uptake kinetics data showed that P-deprived genotype 94D-54 had a little higher As uptake than P-deprived 94D-64, but the difference was not large enough to cause acute toxicity in P-deprived 94D-54. There was no difference in tissue P concentrations between the two genotypes under P deficient conditions. In addition, arsenic speciation in plant tissues (using high performance liquid chromatography-inductively coupled plasma mass spectrometry) was not different between P pretreatments and between genotypes. P-deprived genotype 94D-54 suffered much higher stress induced by arsenate toxicity than P-deprived genotype 94D-64, in terms of lipid peroxidation, tissue H2O2 concentrations and exosmosis of K, P and As. However, P-deprived 94D-54 also had higher overproduction of enzymatic antioxidants (with higher GPX, SOD, CAT) and NPT (non-protein thiols) than P-deprived 94D-64. It appeared that, the higher sensitivity of P-deprived 94D-54 to arsenate toxicity might cause the overproduction of NPT, thus leading to the depletion of GSH and to the accumulation of H2O2. The differential sensitivity of the two genotypes has major implications for breeding rice for As affected paddy soil.",
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T1 - Arsenate causes differential acute toxicity to two P-deprived genotypes of rice seedlings (Oryza sativa L.)

AU - Geng, C N

AU - Zhu, Y G

AU - Hu, Y

AU - Williams, Paul Nicholas

AU - Meharg, A A

PY - 2006

Y1 - 2006

N2 - Significant genotypic difference in response to arsenate toxicity in rice (Oryza sativa) was investigated in root elongation, arsenate uptake kinetics, physiological and biochemical response and arsenic (As) speciation. Uptake kinetics data showed that P-deprived genotype 94D-54 had a little higher As uptake than P-deprived 94D-64, but the difference was not large enough to cause acute toxicity in P-deprived 94D-54. There was no difference in tissue P concentrations between the two genotypes under P deficient conditions. In addition, arsenic speciation in plant tissues (using high performance liquid chromatography-inductively coupled plasma mass spectrometry) was not different between P pretreatments and between genotypes. P-deprived genotype 94D-54 suffered much higher stress induced by arsenate toxicity than P-deprived genotype 94D-64, in terms of lipid peroxidation, tissue H2O2 concentrations and exosmosis of K, P and As. However, P-deprived 94D-54 also had higher overproduction of enzymatic antioxidants (with higher GPX, SOD, CAT) and NPT (non-protein thiols) than P-deprived 94D-64. It appeared that, the higher sensitivity of P-deprived 94D-54 to arsenate toxicity might cause the overproduction of NPT, thus leading to the depletion of GSH and to the accumulation of H2O2. The differential sensitivity of the two genotypes has major implications for breeding rice for As affected paddy soil.

AB - Significant genotypic difference in response to arsenate toxicity in rice (Oryza sativa) was investigated in root elongation, arsenate uptake kinetics, physiological and biochemical response and arsenic (As) speciation. Uptake kinetics data showed that P-deprived genotype 94D-54 had a little higher As uptake than P-deprived 94D-64, but the difference was not large enough to cause acute toxicity in P-deprived 94D-54. There was no difference in tissue P concentrations between the two genotypes under P deficient conditions. In addition, arsenic speciation in plant tissues (using high performance liquid chromatography-inductively coupled plasma mass spectrometry) was not different between P pretreatments and between genotypes. P-deprived genotype 94D-54 suffered much higher stress induced by arsenate toxicity than P-deprived genotype 94D-64, in terms of lipid peroxidation, tissue H2O2 concentrations and exosmosis of K, P and As. However, P-deprived 94D-54 also had higher overproduction of enzymatic antioxidants (with higher GPX, SOD, CAT) and NPT (non-protein thiols) than P-deprived 94D-64. It appeared that, the higher sensitivity of P-deprived 94D-54 to arsenate toxicity might cause the overproduction of NPT, thus leading to the depletion of GSH and to the accumulation of H2O2. The differential sensitivity of the two genotypes has major implications for breeding rice for As affected paddy soil.

KW - acute toxicity

KW - antioxidants

KW - arsenic

KW - rice

KW - stress level

KW - PLASMA-MASS SPECTROMETRY

KW - INDUCED OXIDATIVE STRESS

KW - HOLCUS-LANATUS

KW - ION CHROMATOGRAPHY

KW - CADMIUM TOXICITY

KW - PHOSPHATE-UPTAKE

KW - PLANTS

KW - GLUTATHIONE

KW - PHYTOCHELATINS

KW - TOLERANCE

U2 - 10.1007/s11104-005-1813-7

DO - 10.1007/s11104-005-1813-7

M3 - Article

VL - 279

SP - 297

EP - 306

JO - Communications in Soil Science and Plant Analysis

JF - Communications in Soil Science and Plant Analysis

SN - 0010-3624

ER -