Arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae

J. M. Sharples, Andrew Alexander Meharg, S. M. Chambers, J. W. G. Cairney

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Differential resistance to arsenate (AsO43-) is demonstrated here among populations of the ericoid mycorrhizal fungus Hymenoscyphus ericae isolated from Calluna vulgaris in natural heathland soils and soils contaminated with AsO43-.

Isolates (c. 25) of the fungus from each of two As and Cu mine sites, and a natural heathland site, were screened for AsO43- and Cu2+ resistance by growing isolates in media containing a range of AsO43- and Cu2+ concentrations.

H. ericae populations from the mine sites demonstrated resistance to AsO43- compared with the heathland population; the mine-site populations producing significant growth at the highest AsO43- concentration (4.67 mol m(-3)), whereas growth of the heathland population was almost completely inhibited. EC50 values for mine-site isolates were estimated to be 5-41-times higher than the heathland population. All isolates produced identical responses to increasing Cu2+ concentrations, with no differences observed between mine-site and heathland isolates.

Populations of H. ericae on the contaminated mine sites have developed adaptive resistance to AsO43-. By contrast, Cu2+ resistance appears to be constitutive. (C) New Phytologist (2001) 151: 265-270.

Original languageEnglish
Pages (from-to)265-270
Number of pages5
JournalNew Phytologist
Volume151
DOIs
Publication statusPublished - 2001

Keywords

  • ericoid mycorrhiza
  • Hymenoscyphus ericae
  • arsenate
  • metal tolerance
  • Calluna vulgaris
  • copper
  • HOLCUS-LANATUS L
  • PHOSPHATE-UPTAKE SYSTEM
  • HEAVY-METAL RESISTANCE
  • CALLUNA-VULGARIS
  • PISOLITHUS-TINCTORIUS
  • ECTOMYCORRHIZAL FUNGI
  • SUILLUS-LUTEUS
  • YORKSHIRE FOG
  • TOLERANCE
  • GENETICS

Cite this

Sharples, J. M., Meharg, A. A., Chambers, S. M., & Cairney, J. W. G. (2001). Arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. New Phytologist, 151, 265-270. https://doi.org/10.1046/j.1469-8137.2001.00146.x

Arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. / Sharples, J. M.; Meharg, Andrew Alexander; Chambers, S. M.; Cairney, J. W. G.

In: New Phytologist, Vol. 151, 2001, p. 265-270.

Research output: Contribution to journalArticle

Sharples, J. M. ; Meharg, Andrew Alexander ; Chambers, S. M. ; Cairney, J. W. G. / Arsenate resistance in the ericoid mycorrhizal fungus Hymenoscyphus ericae. In: New Phytologist. 2001 ; Vol. 151. pp. 265-270.
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AB - Differential resistance to arsenate (AsO43-) is demonstrated here among populations of the ericoid mycorrhizal fungus Hymenoscyphus ericae isolated from Calluna vulgaris in natural heathland soils and soils contaminated with AsO43-.Isolates (c. 25) of the fungus from each of two As and Cu mine sites, and a natural heathland site, were screened for AsO43- and Cu2+ resistance by growing isolates in media containing a range of AsO43- and Cu2+ concentrations.H. ericae populations from the mine sites demonstrated resistance to AsO43- compared with the heathland population; the mine-site populations producing significant growth at the highest AsO43- concentration (4.67 mol m(-3)), whereas growth of the heathland population was almost completely inhibited. EC50 values for mine-site isolates were estimated to be 5-41-times higher than the heathland population. All isolates produced identical responses to increasing Cu2+ concentrations, with no differences observed between mine-site and heathland isolates.Populations of H. ericae on the contaminated mine sites have developed adaptive resistance to AsO43-. By contrast, Cu2+ resistance appears to be constitutive. (C) New Phytologist (2001) 151: 265-270.

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