Arsenate sensitivity in ericoid and ectomycorrhizal fungi

J M Sharples, A A Meharg, S M Chambers, J W G Cairney

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Isolates of the endomycorrhizal fungus Hymenoscyphus ericae and the ectomycorrhizal fungus Hebeloma crustuliniforme from soils uncontaminated with AsO43-, were compared with regard to their sensitivity to AsO43- in solution culture. When grown in liquid media amended with a range of AsO43- concentrations, H. ericae demonstrated reduced sensitivity Co AsO43- compared to H. crustuliniforme. The concentrations causing 50% inhibition of growth (EC50) were 1.33 mol/m(3) and 0.33 mol/m(3), respectively, for H. ericae and H. crustuliniforme. The compound AsO43- is a PO43- analogue for the plasmalemma PO43- transporter. The presence of PO43- in the media at high concentrations ameliorated the toxic effects of AsO43- in both the ericoid and ectomycorrhizal fungi. This could be due to: both suppression of the PO43- transporter under high phosporus status and competition of PO43- with AsO43- for the transport protein. The kinetics of AsO43- influx in H. ericae and H. crustuliniforme were also investigated. Hymenoscyphus ericae demonstrated a high K-m value, 0.071 mol/m(3), consistent with values obtained for AsO43--tolerant plants. We suggest that the high K-m value may be a mechanism used by H. ericae to express reduced AsO43- sensitivity. The ecological significance of this reduced sensitivity is also discussed.

Original languageEnglish
Pages (from-to)1848-1855
Number of pages8
JournalEnvironmental Toxicology and Chemistry
Volume18
Issue number8
DOIs
Publication statusPublished - Aug 1999

Keywords

  • arsenate
  • phosphate
  • hymenoscyphus ericae
  • hebeloma crustuliniforme
  • sensitivity
  • holcus-lanatus L
  • phosphate-uptake system
  • cespitosa L beauv
  • pisolithus-tinctorius
  • mycorrhizal infection
  • metal resistance
  • in-vitro
  • tolerance
  • growth
  • transport

Cite this

Sharples, J. M., Meharg, A. A., Chambers, S. M., & Cairney, J. W. G. (1999). Arsenate sensitivity in ericoid and ectomycorrhizal fungi. Environmental Toxicology and Chemistry, 18(8), 1848-1855. https://doi.org/10.1002/etc.5620180836

Arsenate sensitivity in ericoid and ectomycorrhizal fungi. / Sharples, J M ; Meharg, A A ; Chambers, S M ; Cairney, J W G .

In: Environmental Toxicology and Chemistry, Vol. 18, No. 8, 08.1999, p. 1848-1855.

Research output: Contribution to journalArticle

Sharples, JM, Meharg, AA, Chambers, SM & Cairney, JWG 1999, 'Arsenate sensitivity in ericoid and ectomycorrhizal fungi', Environmental Toxicology and Chemistry, vol. 18, no. 8, pp. 1848-1855. https://doi.org/10.1002/etc.5620180836
Sharples, J M ; Meharg, A A ; Chambers, S M ; Cairney, J W G . / Arsenate sensitivity in ericoid and ectomycorrhizal fungi. In: Environmental Toxicology and Chemistry. 1999 ; Vol. 18, No. 8. pp. 1848-1855.
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AB - Isolates of the endomycorrhizal fungus Hymenoscyphus ericae and the ectomycorrhizal fungus Hebeloma crustuliniforme from soils uncontaminated with AsO43-, were compared with regard to their sensitivity to AsO43- in solution culture. When grown in liquid media amended with a range of AsO43- concentrations, H. ericae demonstrated reduced sensitivity Co AsO43- compared to H. crustuliniforme. The concentrations causing 50% inhibition of growth (EC50) were 1.33 mol/m(3) and 0.33 mol/m(3), respectively, for H. ericae and H. crustuliniforme. The compound AsO43- is a PO43- analogue for the plasmalemma PO43- transporter. The presence of PO43- in the media at high concentrations ameliorated the toxic effects of AsO43- in both the ericoid and ectomycorrhizal fungi. This could be due to: both suppression of the PO43- transporter under high phosporus status and competition of PO43- with AsO43- for the transport protein. The kinetics of AsO43- influx in H. ericae and H. crustuliniforme were also investigated. Hymenoscyphus ericae demonstrated a high K-m value, 0.071 mol/m(3), consistent with values obtained for AsO43--tolerant plants. We suggest that the high K-m value may be a mechanism used by H. ericae to express reduced AsO43- sensitivity. The ecological significance of this reduced sensitivity is also discussed.

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KW - hebeloma crustuliniforme

KW - sensitivity

KW - holcus-lanatus L

KW - phosphate-uptake system

KW - cespitosa L beauv

KW - pisolithus-tinctorius

KW - mycorrhizal infection

KW - metal resistance

KW - in-vitro

KW - tolerance

KW - growth

KW - transport

U2 - 10.1002/etc.5620180836

DO - 10.1002/etc.5620180836

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JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

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ER -