Arsenic metabolism in seaweed-eating sheep from Northern Scotland

J Feldmann, K John, P Pengprecha

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

Cation exchange and anion exchange liquid chromatography were coupled to an ICP-MS and optimised for the separation of 13 different arsenic species in body fluids (arsenite, arsenate, dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), trimethylarsine oxide (TMAO), tetramethylarsonium ion (TMA), arsenobetaine (AsB), arsenocholine (AsC), dimethylarsinoyl ethanol (DMAE) and four common dimethylarsinoylribosides (arsenosugars). The arsenic species were de;ermined in seaweed extracts and in the urine and blood serum of seaweed-eating sheep from Northern Scotland. The sheep eat 2-4 kg of seaweed daily which is washed ashore on the most northern Island of Orkney. The urine, blood and wool of 20 North Ronaldsay sheep and kidney, liver and muscle from Il sheep were sampled and analysed for their arsenic species, in addition five Dorset Finn sheep, which lived entirely on grass, were used as a control group. The sheep have a body burden of approximately 45-90 mg arsenic daily. Since the metabolism of arsenic species varies with the arsenite and arsenate being the most toxic, and or,organoarsenic compounds such as arsenobetaine the least toxic compounds, the determination of the arsenic species in the diet and their body fluids are important. The major arsenic species in their diet are arsenoribosides. The major metabolite excreted into urine and blood is DMAA (95 +/- 4.1%) with minor amounts of MMAA, riboside X, TMA and an unidentified species. The occurrence of MMAA is assumed to be a precursor of the exposure to inorganic arsenic, since demethylation of dimethylated or trimethylated organoarsenic compounds is not known (max. MMAA concentration 259 mu g/L). The concentrations in the urine (3179 +/- 2667 mu g/L) and blood (44 +/- 19 mu g/kg) are at least two orders of magnitude higher than the level of arsenic in the urine of the control sheep or literature levels of blood for the unexposed sheep. The tissue samples (liver: 292 +/- 99 mu g/kg kidney: 565 +/- 193 mu g/kg, muscle: 680 +/- 224 mu g/kg) and wool samples (10470 +/- 5690 mu g/kg) show elevated levels which are also 100 times higher than the levels for the unexposed sheep.

Original languageEnglish
Pages (from-to)116-121
Number of pages6
JournalFresenius’ Journal of Analytical Chemistry
Volume368
DOIs
Publication statusPublished - 2000

Keywords

  • SPECIATION
  • URINE
  • INGESTION
  • HERBICIDE
  • MERCURY
  • LIVER
  • GOATS

Cite this

Arsenic metabolism in seaweed-eating sheep from Northern Scotland. / Feldmann, J ; John, K ; Pengprecha, P .

In: Fresenius’ Journal of Analytical Chemistry, Vol. 368, 2000, p. 116-121.

Research output: Contribution to journalArticle

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abstract = "Cation exchange and anion exchange liquid chromatography were coupled to an ICP-MS and optimised for the separation of 13 different arsenic species in body fluids (arsenite, arsenate, dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), trimethylarsine oxide (TMAO), tetramethylarsonium ion (TMA), arsenobetaine (AsB), arsenocholine (AsC), dimethylarsinoyl ethanol (DMAE) and four common dimethylarsinoylribosides (arsenosugars). The arsenic species were de;ermined in seaweed extracts and in the urine and blood serum of seaweed-eating sheep from Northern Scotland. The sheep eat 2-4 kg of seaweed daily which is washed ashore on the most northern Island of Orkney. The urine, blood and wool of 20 North Ronaldsay sheep and kidney, liver and muscle from Il sheep were sampled and analysed for their arsenic species, in addition five Dorset Finn sheep, which lived entirely on grass, were used as a control group. The sheep have a body burden of approximately 45-90 mg arsenic daily. Since the metabolism of arsenic species varies with the arsenite and arsenate being the most toxic, and or,organoarsenic compounds such as arsenobetaine the least toxic compounds, the determination of the arsenic species in the diet and their body fluids are important. The major arsenic species in their diet are arsenoribosides. The major metabolite excreted into urine and blood is DMAA (95 +/- 4.1{\%}) with minor amounts of MMAA, riboside X, TMA and an unidentified species. The occurrence of MMAA is assumed to be a precursor of the exposure to inorganic arsenic, since demethylation of dimethylated or trimethylated organoarsenic compounds is not known (max. MMAA concentration 259 mu g/L). The concentrations in the urine (3179 +/- 2667 mu g/L) and blood (44 +/- 19 mu g/kg) are at least two orders of magnitude higher than the level of arsenic in the urine of the control sheep or literature levels of blood for the unexposed sheep. The tissue samples (liver: 292 +/- 99 mu g/kg kidney: 565 +/- 193 mu g/kg, muscle: 680 +/- 224 mu g/kg) and wool samples (10470 +/- 5690 mu g/kg) show elevated levels which are also 100 times higher than the levels for the unexposed sheep.",
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N2 - Cation exchange and anion exchange liquid chromatography were coupled to an ICP-MS and optimised for the separation of 13 different arsenic species in body fluids (arsenite, arsenate, dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), trimethylarsine oxide (TMAO), tetramethylarsonium ion (TMA), arsenobetaine (AsB), arsenocholine (AsC), dimethylarsinoyl ethanol (DMAE) and four common dimethylarsinoylribosides (arsenosugars). The arsenic species were de;ermined in seaweed extracts and in the urine and blood serum of seaweed-eating sheep from Northern Scotland. The sheep eat 2-4 kg of seaweed daily which is washed ashore on the most northern Island of Orkney. The urine, blood and wool of 20 North Ronaldsay sheep and kidney, liver and muscle from Il sheep were sampled and analysed for their arsenic species, in addition five Dorset Finn sheep, which lived entirely on grass, were used as a control group. The sheep have a body burden of approximately 45-90 mg arsenic daily. Since the metabolism of arsenic species varies with the arsenite and arsenate being the most toxic, and or,organoarsenic compounds such as arsenobetaine the least toxic compounds, the determination of the arsenic species in the diet and their body fluids are important. The major arsenic species in their diet are arsenoribosides. The major metabolite excreted into urine and blood is DMAA (95 +/- 4.1%) with minor amounts of MMAA, riboside X, TMA and an unidentified species. The occurrence of MMAA is assumed to be a precursor of the exposure to inorganic arsenic, since demethylation of dimethylated or trimethylated organoarsenic compounds is not known (max. MMAA concentration 259 mu g/L). The concentrations in the urine (3179 +/- 2667 mu g/L) and blood (44 +/- 19 mu g/kg) are at least two orders of magnitude higher than the level of arsenic in the urine of the control sheep or literature levels of blood for the unexposed sheep. The tissue samples (liver: 292 +/- 99 mu g/kg kidney: 565 +/- 193 mu g/kg, muscle: 680 +/- 224 mu g/kg) and wool samples (10470 +/- 5690 mu g/kg) show elevated levels which are also 100 times higher than the levels for the unexposed sheep.

AB - Cation exchange and anion exchange liquid chromatography were coupled to an ICP-MS and optimised for the separation of 13 different arsenic species in body fluids (arsenite, arsenate, dimethylarsinic acid (DMAA), monomethylarsonic acid (MMAA), trimethylarsine oxide (TMAO), tetramethylarsonium ion (TMA), arsenobetaine (AsB), arsenocholine (AsC), dimethylarsinoyl ethanol (DMAE) and four common dimethylarsinoylribosides (arsenosugars). The arsenic species were de;ermined in seaweed extracts and in the urine and blood serum of seaweed-eating sheep from Northern Scotland. The sheep eat 2-4 kg of seaweed daily which is washed ashore on the most northern Island of Orkney. The urine, blood and wool of 20 North Ronaldsay sheep and kidney, liver and muscle from Il sheep were sampled and analysed for their arsenic species, in addition five Dorset Finn sheep, which lived entirely on grass, were used as a control group. The sheep have a body burden of approximately 45-90 mg arsenic daily. Since the metabolism of arsenic species varies with the arsenite and arsenate being the most toxic, and or,organoarsenic compounds such as arsenobetaine the least toxic compounds, the determination of the arsenic species in the diet and their body fluids are important. The major arsenic species in their diet are arsenoribosides. The major metabolite excreted into urine and blood is DMAA (95 +/- 4.1%) with minor amounts of MMAA, riboside X, TMA and an unidentified species. The occurrence of MMAA is assumed to be a precursor of the exposure to inorganic arsenic, since demethylation of dimethylated or trimethylated organoarsenic compounds is not known (max. MMAA concentration 259 mu g/L). The concentrations in the urine (3179 +/- 2667 mu g/L) and blood (44 +/- 19 mu g/kg) are at least two orders of magnitude higher than the level of arsenic in the urine of the control sheep or literature levels of blood for the unexposed sheep. The tissue samples (liver: 292 +/- 99 mu g/kg kidney: 565 +/- 193 mu g/kg, muscle: 680 +/- 224 mu g/kg) and wool samples (10470 +/- 5690 mu g/kg) show elevated levels which are also 100 times higher than the levels for the unexposed sheep.

KW - SPECIATION

KW - URINE

KW - INGESTION

KW - HERBICIDE

KW - MERCURY

KW - LIVER

KW - GOATS

U2 - 10.1007/s002160000482

DO - 10.1007/s002160000482

M3 - Article

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SP - 116

EP - 121

JO - Fresenius’ Journal of Analytical Chemistry

JF - Fresenius’ Journal of Analytical Chemistry

SN - 0937-0633

ER -