Arsenite transport into paddy rice (Oryza saliva) roots.

Andrew Alexander Meharg

Research output: Contribution to journalArticle

197 Citations (Scopus)

Abstract

Here the mechanism of arsenite transport into paddy rice (Oryza sativa) roots, uptake of which is described by Michaelis-Menten kinetics, is reported. A recent study on yeast (Saccharomyces cerevisiae) showed that undissociated arsenite (its pK(a) is 9.2) was transported across the plasma membrane via a glycerol transporting channel. To investigate whether the same mechanism of transport was involved for rice, competitive studies with glycerol, which is transported into cells via aquaporins, were performed.

Glycerol competed with arsenite for transport in a dose-dependent manner, indicating that arsenite and glycerol uptake mechanisms were the same. Arsenate transport was unaffected by glycerol, confirming that arsenate and arsenite are taken up into cells by different mechanisms.

Antimonite, an arsenite analogue that is transported into S. cerevisiae cells by aquaporins, also competed with arsenite transport in a dose-dependent manner, providing further evidence that arsenite is transported into rice roots via glycerol transporting channels. Mercury (Hg2+) inhibited both arsenite and arsenate uptake, suggesting that inhibition of influx was due to general cellular stress rather than the specific action of Hg2+ on aquaporins.

Arsenite uptake by pea (Pisum sativum) and wheat ( Triticum aestivum) was also described by Michaelis-Menten kinetics.

Original languageEnglish
Pages (from-to)39-44
Number of pages5
JournalNew Phytologist
Volume157
DOIs
Publication statusPublished - 2003

Keywords

  • aquaporins
  • antimonite
  • arsenic
  • arsenite
  • glycerol
  • mercury
  • Oryza sativa
  • rice
  • MEMBRANE AQUAPORIN NTAQP1
  • NICOTIANA-TABACUM
  • GLYCEROL
  • WATER
  • CHANNEL
  • BANGLADESH
  • EXPRESSION
  • TOXICITY
  • PLANTS

Cite this

Arsenite transport into paddy rice (Oryza saliva) roots. / Meharg, Andrew Alexander.

In: New Phytologist, Vol. 157, 2003, p. 39-44.

Research output: Contribution to journalArticle

Meharg, Andrew Alexander. / Arsenite transport into paddy rice (Oryza saliva) roots. In: New Phytologist. 2003 ; Vol. 157. pp. 39-44.
@article{f5ca1f580af14d74956dfbdcafe6f96c,
title = "Arsenite transport into paddy rice (Oryza saliva) roots.",
abstract = "Here the mechanism of arsenite transport into paddy rice (Oryza sativa) roots, uptake of which is described by Michaelis-Menten kinetics, is reported. A recent study on yeast (Saccharomyces cerevisiae) showed that undissociated arsenite (its pK(a) is 9.2) was transported across the plasma membrane via a glycerol transporting channel. To investigate whether the same mechanism of transport was involved for rice, competitive studies with glycerol, which is transported into cells via aquaporins, were performed.Glycerol competed with arsenite for transport in a dose-dependent manner, indicating that arsenite and glycerol uptake mechanisms were the same. Arsenate transport was unaffected by glycerol, confirming that arsenate and arsenite are taken up into cells by different mechanisms.Antimonite, an arsenite analogue that is transported into S. cerevisiae cells by aquaporins, also competed with arsenite transport in a dose-dependent manner, providing further evidence that arsenite is transported into rice roots via glycerol transporting channels. Mercury (Hg2+) inhibited both arsenite and arsenate uptake, suggesting that inhibition of influx was due to general cellular stress rather than the specific action of Hg2+ on aquaporins.Arsenite uptake by pea (Pisum sativum) and wheat ( Triticum aestivum) was also described by Michaelis-Menten kinetics.",
keywords = "aquaporins, antimonite, arsenic, arsenite, glycerol, mercury, Oryza sativa, rice, MEMBRANE AQUAPORIN NTAQP1, NICOTIANA-TABACUM, GLYCEROL, WATER, CHANNEL, BANGLADESH, EXPRESSION, TOXICITY, PLANTS",
author = "Meharg, {Andrew Alexander}",
year = "2003",
doi = "10.1046/j.1469-8137.2003.00655.x",
language = "English",
volume = "157",
pages = "39--44",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley/Blackwell (10.1111)",

}

TY - JOUR

T1 - Arsenite transport into paddy rice (Oryza saliva) roots.

AU - Meharg, Andrew Alexander

PY - 2003

Y1 - 2003

N2 - Here the mechanism of arsenite transport into paddy rice (Oryza sativa) roots, uptake of which is described by Michaelis-Menten kinetics, is reported. A recent study on yeast (Saccharomyces cerevisiae) showed that undissociated arsenite (its pK(a) is 9.2) was transported across the plasma membrane via a glycerol transporting channel. To investigate whether the same mechanism of transport was involved for rice, competitive studies with glycerol, which is transported into cells via aquaporins, were performed.Glycerol competed with arsenite for transport in a dose-dependent manner, indicating that arsenite and glycerol uptake mechanisms were the same. Arsenate transport was unaffected by glycerol, confirming that arsenate and arsenite are taken up into cells by different mechanisms.Antimonite, an arsenite analogue that is transported into S. cerevisiae cells by aquaporins, also competed with arsenite transport in a dose-dependent manner, providing further evidence that arsenite is transported into rice roots via glycerol transporting channels. Mercury (Hg2+) inhibited both arsenite and arsenate uptake, suggesting that inhibition of influx was due to general cellular stress rather than the specific action of Hg2+ on aquaporins.Arsenite uptake by pea (Pisum sativum) and wheat ( Triticum aestivum) was also described by Michaelis-Menten kinetics.

AB - Here the mechanism of arsenite transport into paddy rice (Oryza sativa) roots, uptake of which is described by Michaelis-Menten kinetics, is reported. A recent study on yeast (Saccharomyces cerevisiae) showed that undissociated arsenite (its pK(a) is 9.2) was transported across the plasma membrane via a glycerol transporting channel. To investigate whether the same mechanism of transport was involved for rice, competitive studies with glycerol, which is transported into cells via aquaporins, were performed.Glycerol competed with arsenite for transport in a dose-dependent manner, indicating that arsenite and glycerol uptake mechanisms were the same. Arsenate transport was unaffected by glycerol, confirming that arsenate and arsenite are taken up into cells by different mechanisms.Antimonite, an arsenite analogue that is transported into S. cerevisiae cells by aquaporins, also competed with arsenite transport in a dose-dependent manner, providing further evidence that arsenite is transported into rice roots via glycerol transporting channels. Mercury (Hg2+) inhibited both arsenite and arsenate uptake, suggesting that inhibition of influx was due to general cellular stress rather than the specific action of Hg2+ on aquaporins.Arsenite uptake by pea (Pisum sativum) and wheat ( Triticum aestivum) was also described by Michaelis-Menten kinetics.

KW - aquaporins

KW - antimonite

KW - arsenic

KW - arsenite

KW - glycerol

KW - mercury

KW - Oryza sativa

KW - rice

KW - MEMBRANE AQUAPORIN NTAQP1

KW - NICOTIANA-TABACUM

KW - GLYCEROL

KW - WATER

KW - CHANNEL

KW - BANGLADESH

KW - EXPRESSION

KW - TOXICITY

KW - PLANTS

U2 - 10.1046/j.1469-8137.2003.00655.x

DO - 10.1046/j.1469-8137.2003.00655.x

M3 - Article

VL - 157

SP - 39

EP - 44

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

ER -