Arsenolipids are not uniformly distributed within two brown macroalgal species Saccharina latissima and Alaria esculenta

Ásta H. Pétursdóttir (Corresponding Author), Jonathan Blagden, Karl Gunnarsson, Andrea Raab, Dagmar B. Stengel, Jörg Feldmann (Corresponding Author), Helga Gunnlaugsdottir

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Brown macroalgae Saccharina latissima (30–40 individuals) and Alaria esculenta (15–20 individuals) were collected from natural populations in winter in Iceland. The algal thalli were sectioned into different parts (e.g. holdfast, stipe, old frond, young frond and sori-containing frond sections) that differed in age and biological function. The work elucidated that arsenic (As) was not uniformly distributed within the two brown macroalgal species, with lower levels of total As were found in the stipe/midrib compared to other thallus parts. The arsenosugars mirrored the total arsenic in the seaweed mainly due to AsSugSO3 being the most abundant As species. However, arsenic speciation using parallel HPLC-ICP-MS/ESI-MS elucidated that the arsenic-containing lipids (AsL) had a different distribution where the arsenosugarphospholipids (AsPL) differed by approximately a factor of 4 between the sections containing the lowest and highest concentrations of AsPLs. When placing the sections in order of metabolic activity and an estimate of tissue age, there appeared to be a relationship between the activity and AsPLs, with lower levels of AsPLs in oldest parts. This is the first time such a relationship has been shown for AsLs. Hence, by applying sophisticated analytical techniques, it was possible to gain a deeper understanding of arsenolipids in seaweed.
Original languageEnglish
Pages (from-to)4973-4985
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
Volume411
Issue number19
Early online date31 May 2019
DOIs
Publication statusPublished - Jul 2019

Fingerprint

Arsenic
Seaweed
Iceland
High Pressure Liquid Chromatography
Tissue
Lipids
Population

Keywords

  • Accurate mass
  • Arsenic speciation
  • Identification
  • Mass spectrometry/ICP-MS
  • Quantification
  • Seaweed

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Arsenolipids are not uniformly distributed within two brown macroalgal species Saccharina latissima and Alaria esculenta. / Pétursdóttir, Ásta H. (Corresponding Author); Blagden, Jonathan; Gunnarsson, Karl; Raab, Andrea; Stengel, Dagmar B.; Feldmann, Jörg (Corresponding Author); Gunnlaugsdottir, Helga.

In: Analytical and Bioanalytical Chemistry, Vol. 411, No. 19, 07.2019, p. 4973-4985.

Research output: Contribution to journalArticle

Pétursdóttir, Ásta H. ; Blagden, Jonathan ; Gunnarsson, Karl ; Raab, Andrea ; Stengel, Dagmar B. ; Feldmann, Jörg ; Gunnlaugsdottir, Helga. / Arsenolipids are not uniformly distributed within two brown macroalgal species Saccharina latissima and Alaria esculenta. In: Analytical and Bioanalytical Chemistry. 2019 ; Vol. 411, No. 19. pp. 4973-4985.
@article{ffb4db2907f0463387ee2231849cf092,
title = "Arsenolipids are not uniformly distributed within two brown macroalgal species Saccharina latissima and Alaria esculenta",
abstract = "Brown macroalgae Saccharina latissima (30–40 individuals) and Alaria esculenta (15–20 individuals) were collected from natural populations in winter in Iceland. The algal thalli were sectioned into different parts (e.g. holdfast, stipe, old frond, young frond and sori-containing frond sections) that differed in age and biological function. The work elucidated that arsenic (As) was not uniformly distributed within the two brown macroalgal species, with lower levels of total As were found in the stipe/midrib compared to other thallus parts. The arsenosugars mirrored the total arsenic in the seaweed mainly due to AsSugSO3 being the most abundant As species. However, arsenic speciation using parallel HPLC-ICP-MS/ESI-MS elucidated that the arsenic-containing lipids (AsL) had a different distribution where the arsenosugarphospholipids (AsPL) differed by approximately a factor of 4 between the sections containing the lowest and highest concentrations of AsPLs. When placing the sections in order of metabolic activity and an estimate of tissue age, there appeared to be a relationship between the activity and AsPLs, with lower levels of AsPLs in oldest parts. This is the first time such a relationship has been shown for AsLs. Hence, by applying sophisticated analytical techniques, it was possible to gain a deeper understanding of arsenolipids in seaweed.",
keywords = "Accurate mass, Arsenic speciation, Identification, Mass spectrometry/ICP-MS, Quantification, Seaweed",
author = "P{\'e}tursd{\'o}ttir, {{\'A}sta H.} and Jonathan Blagden and Karl Gunnarsson and Andrea Raab and Stengel, {Dagmar B.} and J{\"o}rg Feldmann and Helga Gunnlaugsdottir",
note = "Open access via Springer Compact Agreement Acknowledgements Emeline Moreira is kindly thanked for her assistance with re-analysing a couple of samples for AsSugar measurement. Johannes Beere is thanked for the analysis on the Orbitrap for batch A. Urd Grandorf Bak is thanked for her helpful advice on seaweed. Funding information This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 656596.",
year = "2019",
month = "7",
doi = "10.1007/s00216-019-01907-x",
language = "English",
volume = "411",
pages = "4973--4985",
journal = "Analytical and Bioanalytical Chemistry",
issn = "1618-2642",
publisher = "Springer Verlag",
number = "19",

}

TY - JOUR

T1 - Arsenolipids are not uniformly distributed within two brown macroalgal species Saccharina latissima and Alaria esculenta

AU - Pétursdóttir, Ásta H.

AU - Blagden, Jonathan

AU - Gunnarsson, Karl

AU - Raab, Andrea

AU - Stengel, Dagmar B.

AU - Feldmann, Jörg

AU - Gunnlaugsdottir, Helga

N1 - Open access via Springer Compact Agreement Acknowledgements Emeline Moreira is kindly thanked for her assistance with re-analysing a couple of samples for AsSugar measurement. Johannes Beere is thanked for the analysis on the Orbitrap for batch A. Urd Grandorf Bak is thanked for her helpful advice on seaweed. Funding information This project has received funding from the European Union’s Horizon 2020 research and innovation programme under the Marie Skłodowska-Curie grant agreement no. 656596.

PY - 2019/7

Y1 - 2019/7

N2 - Brown macroalgae Saccharina latissima (30–40 individuals) and Alaria esculenta (15–20 individuals) were collected from natural populations in winter in Iceland. The algal thalli were sectioned into different parts (e.g. holdfast, stipe, old frond, young frond and sori-containing frond sections) that differed in age and biological function. The work elucidated that arsenic (As) was not uniformly distributed within the two brown macroalgal species, with lower levels of total As were found in the stipe/midrib compared to other thallus parts. The arsenosugars mirrored the total arsenic in the seaweed mainly due to AsSugSO3 being the most abundant As species. However, arsenic speciation using parallel HPLC-ICP-MS/ESI-MS elucidated that the arsenic-containing lipids (AsL) had a different distribution where the arsenosugarphospholipids (AsPL) differed by approximately a factor of 4 between the sections containing the lowest and highest concentrations of AsPLs. When placing the sections in order of metabolic activity and an estimate of tissue age, there appeared to be a relationship between the activity and AsPLs, with lower levels of AsPLs in oldest parts. This is the first time such a relationship has been shown for AsLs. Hence, by applying sophisticated analytical techniques, it was possible to gain a deeper understanding of arsenolipids in seaweed.

AB - Brown macroalgae Saccharina latissima (30–40 individuals) and Alaria esculenta (15–20 individuals) were collected from natural populations in winter in Iceland. The algal thalli were sectioned into different parts (e.g. holdfast, stipe, old frond, young frond and sori-containing frond sections) that differed in age and biological function. The work elucidated that arsenic (As) was not uniformly distributed within the two brown macroalgal species, with lower levels of total As were found in the stipe/midrib compared to other thallus parts. The arsenosugars mirrored the total arsenic in the seaweed mainly due to AsSugSO3 being the most abundant As species. However, arsenic speciation using parallel HPLC-ICP-MS/ESI-MS elucidated that the arsenic-containing lipids (AsL) had a different distribution where the arsenosugarphospholipids (AsPL) differed by approximately a factor of 4 between the sections containing the lowest and highest concentrations of AsPLs. When placing the sections in order of metabolic activity and an estimate of tissue age, there appeared to be a relationship between the activity and AsPLs, with lower levels of AsPLs in oldest parts. This is the first time such a relationship has been shown for AsLs. Hence, by applying sophisticated analytical techniques, it was possible to gain a deeper understanding of arsenolipids in seaweed.

KW - Accurate mass

KW - Arsenic speciation

KW - Identification

KW - Mass spectrometry/ICP-MS

KW - Quantification

KW - Seaweed

UR - http://www.scopus.com/inward/record.url?scp=85066476676&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/arsenolipids-not-uniformly-distributed-within-two-brown-macroalgal-species-saccharina-latissima-alar

U2 - 10.1007/s00216-019-01907-x

DO - 10.1007/s00216-019-01907-x

M3 - Article

VL - 411

SP - 4973

EP - 4985

JO - Analytical and Bioanalytical Chemistry

JF - Analytical and Bioanalytical Chemistry

SN - 1618-2642

IS - 19

ER -