As yet uncultured bacteria phylogenetically classified as Prevotella, Lachnospiraceae incertae sedis and unclassified Bacteroidales, Clostridiales and Ruminococcaceae may play a predominant role in ruminal biohydrogenation

Sharon A. Huws, Eun J. Kim, Michael R. F. Lee, Mark B. Scott, John K S Tweed, Eric Pinloche, R. John Wallace, Nigel D. Scollan

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Microbial biohydrogenation of dietary poly-unsaturated fatty acids (PUFA) to saturated fatty acids (SFA) in the rumen results in the high ratio of SFA/PUFA in ruminant products, such as meat and milk. In vitro, Butyrivibrio proteoclasticus-related bacteria extensively biohydrogenate PUFA to SFA, yet their contribution in the rumen has not been confirmed. The aim of this study was to evaluate the role of Butyrivibrio proteoclasticus group bacteria in ruminal biohydrogenation and to assess the possible role of other bacteria. Fish oil at 0%, 1.5% and 3% dry matter intake was fed to eight Holstein × Friesian steers, in order to elicit changes in the extent of PUFA biohydrogenation. Fatty acid and B. proteoclasticus group 16S rRNA concentrations in rumen digesta were determined. Correlation between digesta 18:0 concentration and B. proteoclasticus group 16S rRNA concentration was low. Terminal restriction fragment length polymorphism and denaturing gradient gel electrophoresis (DGGE) coupled with multivariate statistics revealed that many terminal restriction fragments (T-RFs) and DGGE bands were linked to cis-9, trans-11 conjugated linoleic acid (CLA), 18:1 trans-11 and 18:0 ruminal concentrations. MiCA T-RF predictive identification software showed that these linked T-RFs were likely to originate from as yet uncultured bacteria classified as Prevotella, Lachnospiraceae incertae sedis, and unclassified Bacteroidales, Clostridiales and Ruminococcaceae. Sequencing of linked DGGE bands also revealed that as yet uncultured bacteria classified as Prevotella, Anaerovoax (member of the Lachnospiraceae incertae sedis family), and unclassified Clostridiales and Ruminococcaceae may play a role in biohydrogenation.
Original languageEnglish
Pages (from-to)1500-1512
Number of pages13
JournalEnvironmental Microbiology
Volume13
Issue number6
Early online date21 Mar 2011
DOIs
Publication statusPublished - Jun 2011

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Clostridiales
Prevotella
biohydrogenation
polyunsaturated fatty acids
Butyrivibrio
denaturing gradient gel electrophoresis
Unsaturated Fatty Acids
fatty acid
Denaturing Gradient Gel Electrophoresis
Bacteria
Rumen
saturated fatty acids
Fatty Acids
rumen
bacterium
bacteria
digesta
ribosomal RNA
electrokinesis
rumen bacteria

Keywords

  • animals
  • bacteroidetes
  • base sequence
  • butyrivibrio
  • denaturing gradient gel electrophoresis
  • fatty acids
  • fatty acids, unsaturated
  • fish oils
  • hydrogenation
  • linoleic acids, conjugated
  • molecular sequence data
  • phylogeny
  • prevotella
  • rumen

Cite this

As yet uncultured bacteria phylogenetically classified as Prevotella, Lachnospiraceae incertae sedis and unclassified Bacteroidales, Clostridiales and Ruminococcaceae may play a predominant role in ruminal biohydrogenation. / Huws, Sharon A.; Kim, Eun J.; Lee, Michael R. F.; Scott, Mark B.; Tweed, John K S; Pinloche, Eric; Wallace, R. John; Scollan, Nigel D.

In: Environmental Microbiology, Vol. 13, No. 6, 06.2011, p. 1500-1512.

Research output: Contribution to journalArticle

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AU - Huws, Sharon A.

AU - Kim, Eun J.

AU - Lee, Michael R. F.

AU - Scott, Mark B.

AU - Tweed, John K S

AU - Pinloche, Eric

AU - Wallace, R. John

AU - Scollan, Nigel D.

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KW - bacteroidetes

KW - base sequence

KW - butyrivibrio

KW - denaturing gradient gel electrophoresis

KW - fatty acids

KW - fatty acids, unsaturated

KW - fish oils

KW - hydrogenation

KW - linoleic acids, conjugated

KW - molecular sequence data

KW - phylogeny

KW - prevotella

KW - rumen

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DO - 10.1111/j.1462-2920.2011.02452.x

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EP - 1512

JO - Environmental Microbiology

JF - Environmental Microbiology

SN - 1462-2912

IS - 6

ER -