Ascaridoid nematode infection in haddock (Melanogrammus aeglefmus) and whiting (Merlangius merlangus) in Northeast Atlantic waters

G. J. Pierce*, M. Bao, K. MacKenzie, A. Dunser, L. Giulietti, P. Cipriani, S. Mattiucci, L. C. Hastie

*Corresponding author for this work

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Haddock and whiting are two species of the family Gadidae that are exploited by fisheries mainly in European waters, the former being one of the most important seafood resources in Scotland (UK). The present study aimed to quantify prevalence, abundance and intensity of infection of the zoonotic parasite Anisakis spp. and other ascaridoid nematodes in these fish as part of a study on the risk to consumers. Fish were sourced from research trawling surveys during 2013-2015, aiming to cover a range of size-classes and different times of year. Samples were obtained from the North Sea, west coast of Scotland and the Barents Sea. Fish were stored frozen prior to separation of viscera and fillets and the use of pressing and UV visualisation to locate, identify and count Anisakis spp. and other ascaridoids in fillets (divided into 8 parts), liver and other viscera. A subsample of Anisakis spp. collected from haddock were identified genetically by means of two diagnostic markers (mtDNA cox2 and EF1 alpha - 1 nuclear DNA), and all were identified as A. simplex (s. s.).

Both fish species showed moderate to high infection levels with Anisakis spp. (likely A. simplex (s. s.)) in the visceral organs and cavity, and rather low infection in the musculature. Those A. simplex (s. s.) larvae present in the musculature were mostly found in the anterior ventral area. hi both fish species, the infection rate increased with fish length and in haddock a negative relationship was observed between Anisakis spp. numbers in muscle and body condition.

From our sampling of Anisakis spp. in fish muscle, in Scotland, whiting presents a greater human health risk than haddock in Scotland. The risk arising from consumption of haddock from the Barents Sea (with 72% prevalence of Anisakis) is markedly higher, although in all cases the risk of infection in humans can be minimised if fish are frozen or adequately cooked prior to consumption. We present previously unpublished information on reported cases of anisakiasis in the United Kingdom during 2000-2013, which included 4 cases from Scotland among 22 overall. While these findings suggest that anisakiasis is relatively rare in the UK, underreporting is likely and further investigation of the incidence of anisakiasis is needed.

Original languageEnglish
Pages (from-to)122-133
Number of pages12
JournalFisheries Research
Volume202
Early online date28 Sep 2017
DOIs
Publication statusPublished - 30 Jun 2018

Keywords

  • Anisakis simplex (s. s.)
  • Zoonotic parasite
  • Haddock
  • Whiting
  • Infection levels
  • WHALEWORM ANISAKIS-SIMPLEX
  • HERRING CLUPEA-HARENGUS
  • COD GADUS-MORHUA
  • MERLUCCIUS-MERLUCCIUS
  • PEPSIN TREATMENTS
  • LARVAL NEMATODES
  • FISHERY PRODUCTS
  • 3RD-STAGE LARVAE
  • SCOTTISH WATERS
  • BARENTS SEA

Cite this

Ascaridoid nematode infection in haddock (Melanogrammus aeglefmus) and whiting (Merlangius merlangus) in Northeast Atlantic waters. / Pierce, G. J.; Bao, M.; MacKenzie, K.; Dunser, A.; Giulietti, L.; Cipriani, P.; Mattiucci, S.; Hastie, L. C.

In: Fisheries Research, Vol. 202, 30.06.2018, p. 122-133.

Research output: Contribution to journalArticle

Pierce, G. J. ; Bao, M. ; MacKenzie, K. ; Dunser, A. ; Giulietti, L. ; Cipriani, P. ; Mattiucci, S. ; Hastie, L. C. / Ascaridoid nematode infection in haddock (Melanogrammus aeglefmus) and whiting (Merlangius merlangus) in Northeast Atlantic waters. In: Fisheries Research. 2018 ; Vol. 202. pp. 122-133.
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title = "Ascaridoid nematode infection in haddock (Melanogrammus aeglefmus) and whiting (Merlangius merlangus) in Northeast Atlantic waters",
abstract = "Haddock and whiting are two species of the family Gadidae that are exploited by fisheries mainly in European waters, the former being one of the most important seafood resources in Scotland (UK). The present study aimed to quantify prevalence, abundance and intensity of infection of the zoonotic parasite Anisakis spp. and other ascaridoid nematodes in these fish as part of a study on the risk to consumers. Fish were sourced from research trawling surveys during 2013-2015, aiming to cover a range of size-classes and different times of year. Samples were obtained from the North Sea, west coast of Scotland and the Barents Sea. Fish were stored frozen prior to separation of viscera and fillets and the use of pressing and UV visualisation to locate, identify and count Anisakis spp. and other ascaridoids in fillets (divided into 8 parts), liver and other viscera. A subsample of Anisakis spp. collected from haddock were identified genetically by means of two diagnostic markers (mtDNA cox2 and EF1 alpha - 1 nuclear DNA), and all were identified as A. simplex (s. s.).Both fish species showed moderate to high infection levels with Anisakis spp. (likely A. simplex (s. s.)) in the visceral organs and cavity, and rather low infection in the musculature. Those A. simplex (s. s.) larvae present in the musculature were mostly found in the anterior ventral area. hi both fish species, the infection rate increased with fish length and in haddock a negative relationship was observed between Anisakis spp. numbers in muscle and body condition.From our sampling of Anisakis spp. in fish muscle, in Scotland, whiting presents a greater human health risk than haddock in Scotland. The risk arising from consumption of haddock from the Barents Sea (with 72{\%} prevalence of Anisakis) is markedly higher, although in all cases the risk of infection in humans can be minimised if fish are frozen or adequately cooked prior to consumption. We present previously unpublished information on reported cases of anisakiasis in the United Kingdom during 2000-2013, which included 4 cases from Scotland among 22 overall. While these findings suggest that anisakiasis is relatively rare in the UK, underreporting is likely and further investigation of the incidence of anisakiasis is needed.",
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note = "The present work was supported by the European Union's 7th Framework Programme for Research, Technological Development and Demonstration, project Parasite risk assessment with integrated tools in EU fish production value chains (PARASITE), Grant Agreement (GA) no. 312068. GJP acknowledges additional support from the University of Aveiro and Caixa Geral de Dep{\'o}sitos (Portugal). We thank our colleagues from Marine Scotland Science for supplying samples from Scottish research surveys, and colleagues from HERMES and NIFES (especially Arne Levsen, who also reviewed a draft of the manuscript) for sampling and providing data for haddock in the Barents Sea. Claire Alexander at the Scottish Parasite Diagnostic and Reference Laboratory and Ivelina Yonova at the RCGP Research and Surveillance Centre kindly provided the information on the number of anisakiasis cases recorded in the UK. We also thank Jianjun Wang for helping us to source UK human infection data and Fedor Lishchenko for checking Russian literature.",
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T1 - Ascaridoid nematode infection in haddock (Melanogrammus aeglefmus) and whiting (Merlangius merlangus) in Northeast Atlantic waters

AU - Pierce, G. J.

AU - Bao, M.

AU - MacKenzie, K.

AU - Dunser, A.

AU - Giulietti, L.

AU - Cipriani, P.

AU - Mattiucci, S.

AU - Hastie, L. C.

N1 - The present work was supported by the European Union's 7th Framework Programme for Research, Technological Development and Demonstration, project Parasite risk assessment with integrated tools in EU fish production value chains (PARASITE), Grant Agreement (GA) no. 312068. GJP acknowledges additional support from the University of Aveiro and Caixa Geral de Depósitos (Portugal). We thank our colleagues from Marine Scotland Science for supplying samples from Scottish research surveys, and colleagues from HERMES and NIFES (especially Arne Levsen, who also reviewed a draft of the manuscript) for sampling and providing data for haddock in the Barents Sea. Claire Alexander at the Scottish Parasite Diagnostic and Reference Laboratory and Ivelina Yonova at the RCGP Research and Surveillance Centre kindly provided the information on the number of anisakiasis cases recorded in the UK. We also thank Jianjun Wang for helping us to source UK human infection data and Fedor Lishchenko for checking Russian literature.

PY - 2018/6/30

Y1 - 2018/6/30

N2 - Haddock and whiting are two species of the family Gadidae that are exploited by fisheries mainly in European waters, the former being one of the most important seafood resources in Scotland (UK). The present study aimed to quantify prevalence, abundance and intensity of infection of the zoonotic parasite Anisakis spp. and other ascaridoid nematodes in these fish as part of a study on the risk to consumers. Fish were sourced from research trawling surveys during 2013-2015, aiming to cover a range of size-classes and different times of year. Samples were obtained from the North Sea, west coast of Scotland and the Barents Sea. Fish were stored frozen prior to separation of viscera and fillets and the use of pressing and UV visualisation to locate, identify and count Anisakis spp. and other ascaridoids in fillets (divided into 8 parts), liver and other viscera. A subsample of Anisakis spp. collected from haddock were identified genetically by means of two diagnostic markers (mtDNA cox2 and EF1 alpha - 1 nuclear DNA), and all were identified as A. simplex (s. s.).Both fish species showed moderate to high infection levels with Anisakis spp. (likely A. simplex (s. s.)) in the visceral organs and cavity, and rather low infection in the musculature. Those A. simplex (s. s.) larvae present in the musculature were mostly found in the anterior ventral area. hi both fish species, the infection rate increased with fish length and in haddock a negative relationship was observed between Anisakis spp. numbers in muscle and body condition.From our sampling of Anisakis spp. in fish muscle, in Scotland, whiting presents a greater human health risk than haddock in Scotland. The risk arising from consumption of haddock from the Barents Sea (with 72% prevalence of Anisakis) is markedly higher, although in all cases the risk of infection in humans can be minimised if fish are frozen or adequately cooked prior to consumption. We present previously unpublished information on reported cases of anisakiasis in the United Kingdom during 2000-2013, which included 4 cases from Scotland among 22 overall. While these findings suggest that anisakiasis is relatively rare in the UK, underreporting is likely and further investigation of the incidence of anisakiasis is needed.

AB - Haddock and whiting are two species of the family Gadidae that are exploited by fisheries mainly in European waters, the former being one of the most important seafood resources in Scotland (UK). The present study aimed to quantify prevalence, abundance and intensity of infection of the zoonotic parasite Anisakis spp. and other ascaridoid nematodes in these fish as part of a study on the risk to consumers. Fish were sourced from research trawling surveys during 2013-2015, aiming to cover a range of size-classes and different times of year. Samples were obtained from the North Sea, west coast of Scotland and the Barents Sea. Fish were stored frozen prior to separation of viscera and fillets and the use of pressing and UV visualisation to locate, identify and count Anisakis spp. and other ascaridoids in fillets (divided into 8 parts), liver and other viscera. A subsample of Anisakis spp. collected from haddock were identified genetically by means of two diagnostic markers (mtDNA cox2 and EF1 alpha - 1 nuclear DNA), and all were identified as A. simplex (s. s.).Both fish species showed moderate to high infection levels with Anisakis spp. (likely A. simplex (s. s.)) in the visceral organs and cavity, and rather low infection in the musculature. Those A. simplex (s. s.) larvae present in the musculature were mostly found in the anterior ventral area. hi both fish species, the infection rate increased with fish length and in haddock a negative relationship was observed between Anisakis spp. numbers in muscle and body condition.From our sampling of Anisakis spp. in fish muscle, in Scotland, whiting presents a greater human health risk than haddock in Scotland. The risk arising from consumption of haddock from the Barents Sea (with 72% prevalence of Anisakis) is markedly higher, although in all cases the risk of infection in humans can be minimised if fish are frozen or adequately cooked prior to consumption. We present previously unpublished information on reported cases of anisakiasis in the United Kingdom during 2000-2013, which included 4 cases from Scotland among 22 overall. While these findings suggest that anisakiasis is relatively rare in the UK, underreporting is likely and further investigation of the incidence of anisakiasis is needed.

KW - Anisakis simplex (s. s.)

KW - Zoonotic parasite

KW - Haddock

KW - Whiting

KW - Infection levels

KW - WHALEWORM ANISAKIS-SIMPLEX

KW - HERRING CLUPEA-HARENGUS

KW - COD GADUS-MORHUA

KW - MERLUCCIUS-MERLUCCIUS

KW - PEPSIN TREATMENTS

KW - LARVAL NEMATODES

KW - FISHERY PRODUCTS

KW - 3RD-STAGE LARVAE

KW - SCOTTISH WATERS

KW - BARENTS SEA

U2 - 10.1016/j.fishres.2017.09.008

DO - 10.1016/j.fishres.2017.09.008

M3 - Article

VL - 202

SP - 122

EP - 133

JO - Fisheries Research

JF - Fisheries Research

SN - 0165-7836

ER -