Assessment of animal hosts of pathogenic Leptospira in northern Tanzania

Kathryn J. Allan*, Jo E.B. Halliday, Mark Moseley, Ryan W. Carter, Ahmed Ahmed, Marga G.A. Goris, Rudy A. Hartskeerl, Julius Keyyu, Tito Kibona, Venance P. Maro, Michael J. Maze, Blandina T. Mmbaga, Rigobert Tarimo, John A. Crump, Sarah Cleaveland

*Corresponding author for this work

Research output: Contribution to journalArticle

9 Citations (Scopus)
5 Downloads (Pure)

Abstract

Leptospirosis is a zoonotic bacterial disease that affects more than one million people worldwide each year. Human infection is acquired through direct or indirect contact with the urine of an infected animal. A wide range of animals including rodents and livestock may shed Leptospira bacteria and act as a source of infection for people. In the Kilimanjaro Region of northern Tanzania, leptospirosis is an important cause of acute febrile illness, yet relatively little is known about animal hosts of Leptospira infection in this area. The roles of rodents and ruminant livestock in the epidemiology of leptospirosis were evaluated through two linked studies. A cross-sectional study of peri-domestic rodents performed in two districts with a high reported incidence of human leptospirosis found no evidence of Leptospira infection among rodent species trapped in and around randomly selected households. In contrast, pathogenic Leptospira infection was detected in 7.08% cattle (n = 452 [5.1–9.8%]), 1.20% goats (n = 167 [0.3–4.3%]) and 1.12% sheep (n = 89 [0.1–60.0%]) sampled in local slaughterhouses. Four Leptospira genotypes were detected in livestock. Two distinct clades of L. borgpetersenii were identified in cattle as well as a clade of novel secY sequences that showed only 95% identity to known Leptospira sequences. Identical L. kirschneri sequences were obtained from qPCR-positive kidney samples from cattle, sheep and goats. These results indicate that ruminant livestock are important hosts of Leptospira in northern Tanzania. Infected livestock may act as a source of Leptospira infection for people. Additional work is needed to understand the role of livestock in the maintenance and transmission of Leptospira infection in this region and to examine linkages between human and livestock infections.

Original languageEnglish
Article numbere0006444
JournalPLoS Neglected Tropical Diseases
Volume12
Issue number6
DOIs
Publication statusPublished - 7 Jun 2018

Fingerprint

Leptospira
Tanzania
Livestock
Leptospirosis
Infection
Rodentia
Ruminants
Goats
Sheep
Abattoirs
Infectious Disease Transmission
Zoonoses
Epidemiology
Fever
Cross-Sectional Studies
Genotype
Maintenance
Urine
Bacteria
Kidney

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Allan, K. J., Halliday, J. E. B., Moseley, M., Carter, R. W., Ahmed, A., Goris, M. G. A., ... Cleaveland, S. (2018). Assessment of animal hosts of pathogenic Leptospira in northern Tanzania. PLoS Neglected Tropical Diseases, 12(6), [e0006444]. https://doi.org/10.1371/journal.pntd.0006444

Assessment of animal hosts of pathogenic Leptospira in northern Tanzania. / Allan, Kathryn J.; Halliday, Jo E.B.; Moseley, Mark; Carter, Ryan W.; Ahmed, Ahmed; Goris, Marga G.A.; Hartskeerl, Rudy A.; Keyyu, Julius; Kibona, Tito; Maro, Venance P.; Maze, Michael J.; Mmbaga, Blandina T.; Tarimo, Rigobert; Crump, John A.; Cleaveland, Sarah.

In: PLoS Neglected Tropical Diseases, Vol. 12, No. 6, e0006444, 07.06.2018.

Research output: Contribution to journalArticle

Allan, KJ, Halliday, JEB, Moseley, M, Carter, RW, Ahmed, A, Goris, MGA, Hartskeerl, RA, Keyyu, J, Kibona, T, Maro, VP, Maze, MJ, Mmbaga, BT, Tarimo, R, Crump, JA & Cleaveland, S 2018, 'Assessment of animal hosts of pathogenic Leptospira in northern Tanzania', PLoS Neglected Tropical Diseases, vol. 12, no. 6, e0006444. https://doi.org/10.1371/journal.pntd.0006444
Allan KJ, Halliday JEB, Moseley M, Carter RW, Ahmed A, Goris MGA et al. Assessment of animal hosts of pathogenic Leptospira in northern Tanzania. PLoS Neglected Tropical Diseases. 2018 Jun 7;12(6). e0006444. https://doi.org/10.1371/journal.pntd.0006444
Allan, Kathryn J. ; Halliday, Jo E.B. ; Moseley, Mark ; Carter, Ryan W. ; Ahmed, Ahmed ; Goris, Marga G.A. ; Hartskeerl, Rudy A. ; Keyyu, Julius ; Kibona, Tito ; Maro, Venance P. ; Maze, Michael J. ; Mmbaga, Blandina T. ; Tarimo, Rigobert ; Crump, John A. ; Cleaveland, Sarah. / Assessment of animal hosts of pathogenic Leptospira in northern Tanzania. In: PLoS Neglected Tropical Diseases. 2018 ; Vol. 12, No. 6.
@article{14c8ec8828444ada945fbb2e9dbcab69,
title = "Assessment of animal hosts of pathogenic Leptospira in northern Tanzania",
abstract = "Leptospirosis is a zoonotic bacterial disease that affects more than one million people worldwide each year. Human infection is acquired through direct or indirect contact with the urine of an infected animal. A wide range of animals including rodents and livestock may shed Leptospira bacteria and act as a source of infection for people. In the Kilimanjaro Region of northern Tanzania, leptospirosis is an important cause of acute febrile illness, yet relatively little is known about animal hosts of Leptospira infection in this area. The roles of rodents and ruminant livestock in the epidemiology of leptospirosis were evaluated through two linked studies. A cross-sectional study of peri-domestic rodents performed in two districts with a high reported incidence of human leptospirosis found no evidence of Leptospira infection among rodent species trapped in and around randomly selected households. In contrast, pathogenic Leptospira infection was detected in 7.08{\%} cattle (n = 452 [5.1–9.8{\%}]), 1.20{\%} goats (n = 167 [0.3–4.3{\%}]) and 1.12{\%} sheep (n = 89 [0.1–60.0{\%}]) sampled in local slaughterhouses. Four Leptospira genotypes were detected in livestock. Two distinct clades of L. borgpetersenii were identified in cattle as well as a clade of novel secY sequences that showed only 95{\%} identity to known Leptospira sequences. Identical L. kirschneri sequences were obtained from qPCR-positive kidney samples from cattle, sheep and goats. These results indicate that ruminant livestock are important hosts of Leptospira in northern Tanzania. Infected livestock may act as a source of Leptospira infection for people. Additional work is needed to understand the role of livestock in the maintenance and transmission of Leptospira infection in this region and to examine linkages between human and livestock infections.",
author = "Allan, {Kathryn J.} and Halliday, {Jo E.B.} and Mark Moseley and Carter, {Ryan W.} and Ahmed Ahmed and Goris, {Marga G.A.} and Hartskeerl, {Rudy A.} and Julius Keyyu and Tito Kibona and Maro, {Venance P.} and Maze, {Michael J.} and Mmbaga, {Blandina T.} and Rigobert Tarimo and Crump, {John A.} and Sarah Cleaveland",
note = "Funding: This work was supported by the Wellcome Trust (grant number 096400/Z/11/Z; https://wellcome.ac.uk/). JEBH, VPM, JAC, and SC received support from the Research Councils UK, UK Department for International Development, and UK Biotechnology and Biological Sciences Research Council (BBSRC) (grant numbers BB/J010367/1, BB/L018926, BB/L017679, BB/L018845; http://www.bbsrc.ac.uk/). JAC and VPM also received support from the US National Institutes of Health (NIH)-National Science Foundation (NSF) Ecology and Evolution of Infectious Disease program (R01TW009237; https://www.fic.nih.gov/programs/pages/ecology-infectious-diseases.aspx). MM received support from the BBSRC East of Scotland Bioscience Doctoral Training Partnership (http://www.eastscotbiodtp.ac.uk/). MJM received support from a University of Otago Frances G. Cotter Scholarship and a University of Otago MacGibbon PhD Travel Fellowship (http://www.otago.ac.nz/). VPM and JAC received support from the US National Institutes of Health National Institute for Allergy and Infectious (grant number R01 AI121378; https://www.niaid.nih.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Data Availability: Datasets supporting this manuscript are available through: http://dx.doi.org/10.5525/gla.researchdata.582. Unique sequences generated through this study are available through GenBank (accession numbers MF955862 to MF955882).",
year = "2018",
month = "6",
day = "7",
doi = "10.1371/journal.pntd.0006444",
language = "English",
volume = "12",
journal = "PLoS Neglected Tropical Diseases",
issn = "1935-2735",
publisher = "Public Library of Science",
number = "6",

}

TY - JOUR

T1 - Assessment of animal hosts of pathogenic Leptospira in northern Tanzania

AU - Allan, Kathryn J.

AU - Halliday, Jo E.B.

AU - Moseley, Mark

AU - Carter, Ryan W.

AU - Ahmed, Ahmed

AU - Goris, Marga G.A.

AU - Hartskeerl, Rudy A.

AU - Keyyu, Julius

AU - Kibona, Tito

AU - Maro, Venance P.

AU - Maze, Michael J.

AU - Mmbaga, Blandina T.

AU - Tarimo, Rigobert

AU - Crump, John A.

AU - Cleaveland, Sarah

N1 - Funding: This work was supported by the Wellcome Trust (grant number 096400/Z/11/Z; https://wellcome.ac.uk/). JEBH, VPM, JAC, and SC received support from the Research Councils UK, UK Department for International Development, and UK Biotechnology and Biological Sciences Research Council (BBSRC) (grant numbers BB/J010367/1, BB/L018926, BB/L017679, BB/L018845; http://www.bbsrc.ac.uk/). JAC and VPM also received support from the US National Institutes of Health (NIH)-National Science Foundation (NSF) Ecology and Evolution of Infectious Disease program (R01TW009237; https://www.fic.nih.gov/programs/pages/ecology-infectious-diseases.aspx). MM received support from the BBSRC East of Scotland Bioscience Doctoral Training Partnership (http://www.eastscotbiodtp.ac.uk/). MJM received support from a University of Otago Frances G. Cotter Scholarship and a University of Otago MacGibbon PhD Travel Fellowship (http://www.otago.ac.nz/). VPM and JAC received support from the US National Institutes of Health National Institute for Allergy and Infectious (grant number R01 AI121378; https://www.niaid.nih.gov/). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Data Availability: Datasets supporting this manuscript are available through: http://dx.doi.org/10.5525/gla.researchdata.582. Unique sequences generated through this study are available through GenBank (accession numbers MF955862 to MF955882).

PY - 2018/6/7

Y1 - 2018/6/7

N2 - Leptospirosis is a zoonotic bacterial disease that affects more than one million people worldwide each year. Human infection is acquired through direct or indirect contact with the urine of an infected animal. A wide range of animals including rodents and livestock may shed Leptospira bacteria and act as a source of infection for people. In the Kilimanjaro Region of northern Tanzania, leptospirosis is an important cause of acute febrile illness, yet relatively little is known about animal hosts of Leptospira infection in this area. The roles of rodents and ruminant livestock in the epidemiology of leptospirosis were evaluated through two linked studies. A cross-sectional study of peri-domestic rodents performed in two districts with a high reported incidence of human leptospirosis found no evidence of Leptospira infection among rodent species trapped in and around randomly selected households. In contrast, pathogenic Leptospira infection was detected in 7.08% cattle (n = 452 [5.1–9.8%]), 1.20% goats (n = 167 [0.3–4.3%]) and 1.12% sheep (n = 89 [0.1–60.0%]) sampled in local slaughterhouses. Four Leptospira genotypes were detected in livestock. Two distinct clades of L. borgpetersenii were identified in cattle as well as a clade of novel secY sequences that showed only 95% identity to known Leptospira sequences. Identical L. kirschneri sequences were obtained from qPCR-positive kidney samples from cattle, sheep and goats. These results indicate that ruminant livestock are important hosts of Leptospira in northern Tanzania. Infected livestock may act as a source of Leptospira infection for people. Additional work is needed to understand the role of livestock in the maintenance and transmission of Leptospira infection in this region and to examine linkages between human and livestock infections.

AB - Leptospirosis is a zoonotic bacterial disease that affects more than one million people worldwide each year. Human infection is acquired through direct or indirect contact with the urine of an infected animal. A wide range of animals including rodents and livestock may shed Leptospira bacteria and act as a source of infection for people. In the Kilimanjaro Region of northern Tanzania, leptospirosis is an important cause of acute febrile illness, yet relatively little is known about animal hosts of Leptospira infection in this area. The roles of rodents and ruminant livestock in the epidemiology of leptospirosis were evaluated through two linked studies. A cross-sectional study of peri-domestic rodents performed in two districts with a high reported incidence of human leptospirosis found no evidence of Leptospira infection among rodent species trapped in and around randomly selected households. In contrast, pathogenic Leptospira infection was detected in 7.08% cattle (n = 452 [5.1–9.8%]), 1.20% goats (n = 167 [0.3–4.3%]) and 1.12% sheep (n = 89 [0.1–60.0%]) sampled in local slaughterhouses. Four Leptospira genotypes were detected in livestock. Two distinct clades of L. borgpetersenii were identified in cattle as well as a clade of novel secY sequences that showed only 95% identity to known Leptospira sequences. Identical L. kirschneri sequences were obtained from qPCR-positive kidney samples from cattle, sheep and goats. These results indicate that ruminant livestock are important hosts of Leptospira in northern Tanzania. Infected livestock may act as a source of Leptospira infection for people. Additional work is needed to understand the role of livestock in the maintenance and transmission of Leptospira infection in this region and to examine linkages between human and livestock infections.

UR - http://www.scopus.com/inward/record.url?scp=85049026585&partnerID=8YFLogxK

U2 - 10.1371/journal.pntd.0006444

DO - 10.1371/journal.pntd.0006444

M3 - Article

VL - 12

JO - PLoS Neglected Tropical Diseases

JF - PLoS Neglected Tropical Diseases

SN - 1935-2735

IS - 6

M1 - e0006444

ER -