Assessment of metabolic diversity within the intestinal microbiota from healthy humans using combined molecular and cultural approaches

Christophe Chassard, Karen P. Scott, Perrine Marquet, Jennifer C. Martin, Christophe Del'homme, Michel Dapoigny, Harry J. Flint, Annick Bernalier-Donadille

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The human gut harbours a wide range of bacterial communities that play key roles in supplying nutrients and energy to the host through anaerobic fermentation of dietary components and host secretions. This fermentative process involves different functional groups of microorganisms linked in a trophic chain. Although the diversity of the intestinal microbiota has been studied extensively using molecular techniques, the functional aspects of this biodiversity remain mostly unexplored. The aim of the present work was to enumerate the principal metabolic groups of microorganisms involved in the fermentative process in the gut of healthy humans. These functional groups of microorganisms were quantified by a cultural approach, while the taxonomic composition of the microbiota was assessed by in situ hybridization on the same faecal samples. The functional groups of microorganisms that predominated in the gut were the polysaccharide-degrading populations involved in the breakdown of the most readily available exogenous and endogenous substrates and the predominant butyrate-producing species. Most of the functional groups of microorganisms studied appeared to be present at rather similar levels in all healthy volunteers, suggesting that optimal numbers of these various bacterial groups are crucial for efficient gut fermentation, as well as for host nutrition and health. Significant interindividual differences were, however, confirmed with respect to the numbers of methanogenic archaea, filter paper-degrading and acetogenic bacteria and the products formed by lactate-utilizing bacteria.

Original languageEnglish
Pages (from-to)496-504
Number of pages9
JournalFEMS Microbiology Ecology
Volume66
Issue number3
Early online date22 Sep 2008
DOIs
Publication statusPublished - Dec 2008

Keywords

  • human
  • gut microbiota
  • fermentation process
  • metabolic diversity
  • in situ hybridization
  • cultural approaches
  • sulfate-reducing bacteria
  • in-situ hybridization
  • targeted oligonucleotide probes
  • butyrate-producing bacteria
  • human fecal bacteria
  • RNA-based probes
  • human feces
  • human colon
  • human gut
  • SP-NOV.
  • human

Cite this

Assessment of metabolic diversity within the intestinal microbiota from healthy humans using combined molecular and cultural approaches. / Chassard, Christophe; Scott, Karen P.; Marquet, Perrine; Martin, Jennifer C.; Del'homme, Christophe; Dapoigny, Michel; Flint, Harry J.; Bernalier-Donadille, Annick.

In: FEMS Microbiology Ecology, Vol. 66, No. 3, 12.2008, p. 496-504.

Research output: Contribution to journalArticle

Chassard, Christophe ; Scott, Karen P. ; Marquet, Perrine ; Martin, Jennifer C. ; Del'homme, Christophe ; Dapoigny, Michel ; Flint, Harry J. ; Bernalier-Donadille, Annick. / Assessment of metabolic diversity within the intestinal microbiota from healthy humans using combined molecular and cultural approaches. In: FEMS Microbiology Ecology. 2008 ; Vol. 66, No. 3. pp. 496-504.
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