Assessment of microbial diversity in human colonic samples by 16S rDNA sequence analysis

Georgina Louise Hold, S. E. Pryde, V. J. Russell, E. Furrie, Harry James Flint

Research output: Contribution to journalArticle

261 Citations (Scopus)

Abstract

The bacterial species diversity of three colonic tissue samples from elderly people was investigated by sequence analysis of randomly cloned eubacterial 16S rDNA. The majority of sequences (87%) clustered within three bacterial groups: (1) Bacteroides; (2) low G+C content Gram-positives related to Clostridium coccoides (cluster XIVa); (3) Gram-positives related to Clostridium leptum (cluster IV). These groups have been shown to dominate the human faecal flora. Only 25%, of sequences were closely related (> 97%) to current species type strains, and 28% were less than 97%, related to any database entry. 19% of sequences were most closely related to recently isolated butyrate-producing bacteria belonging to clusters XIVa and IV, with a further 18% of the sequences most closely related to Ruminococcus obeum and Ruminococcus torques (members of cluster XIVa). These results provide the First molecular information on the microbial diversity present in human colonic samples. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)33-39
Number of pages6
JournalFEMS Microbiology Ecology
Volume39
Issue number1
DOIs
Publication statusPublished - Jan 2002

Keywords

  • colonic sample
  • 16S rDNA sequencing
  • microbial diversity
  • targeted oligonucleotide probes
  • ribosomal database project
  • In-situ hybridization
  • human fecal samples
  • bacterial-populations
  • human feces
  • human gut
  • RNA
  • quantification
  • communities

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