Assessment of microplastic-sorbed contaminant bioavailability through analysis of biomarker gene expression in larval zebrafish

Victoria A. Sleight; Adil Bakir; Richard C. Thompson; Theodore B. Henry

doi:10.1016/j.marpolbul.2016.12.055

Assessment of microplastic-sorbed contaminant bioavailability through analysis of biomarker gene expression in larval zebrafish

Victoria A. Sleight^* (Corresponding Author), Adil Bakir, Richard C. Thompson, Theodore B. Henry

^*Corresponding author for this work

Aberdeen Centre For Environmental Sustainability

Research output: Contribution to journal › Article › peer-review

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Abstract

Microplastics (MPs) are prevalent in marine ecosystems. Because toxicants (termed here “co-contaminants”) can sorb to MPs, there is potential for MPs to alter co-contaminant bioavailability. Our objective was to demonstrate sorption of two co-contaminants with different physicochemistries [phenanthrene (Phe), log10Kow = 4.57; and 17α-ethinylestradiol (EE2), log10Kow = 3.67] to MPs; and assess whether co-contaminant bioavailability was increased after MP settlement. Bioavailability was indicated by gene expression in larval zebrafish. Both Phe and EE2 sorbed to MPs, which reduced bioavailability by a maximum of 33% and 48% respectively. Sorption occurred, but was not consistent with predictions based on co-contaminant physicochemistry (Phe having higher log10Kow was expected to have higher sorption). Contaminated MPs settled to the bottom of the exposures did not lead to increased bioavailability of Phe or EE2. Phe was 48% more bioavailable than predicted by a linear sorption model, organism-based measurements therefore contribute unique insight into MP co-contaminant bioavailability.

Original language	English
Pages (from-to)	291-297
Number of pages	7
Journal	Marine Pollution Bulletin
Volume	116
Issue number	1-2
Early online date	12 Jan 2017
DOIs	https://doi.org/10.1016/j.marpolbul.2016.12.055
Publication status	Published - 15 Mar 2017

Bibliographical note

Acknowledgements
Thank you to Stanley McMahon for fish maintenance and husbandry in the Zebrafish Research Facility, Plymouth, and Andrew Atfield and Dr. Helena Reinardy for laboratory assistance.

Keywords

Danio rerio
microplastics
phenanthrene
17α-ethinylestradiol
sorption
bioavailability

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.marpolbul.2016.12.055Licence: CC BY

Sleight_etal_MPB_Assessment_VOR
© 2016 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/)
Final published version, 836 KBLicence: CC BY

Victoria Sleight
- Biological Sciences, Aberdeen Centre For Environmental Sustainability - Senior Lecturer
Person: Academic

Cite this

@article{e170c372f6294badbec0364638d7f732,

title = "Assessment of microplastic-sorbed contaminant bioavailability through analysis of biomarker gene expression in larval zebrafish",

abstract = "Microplastics (MPs) are prevalent in marine ecosystems. Because toxicants (termed here “co-contaminants”) can sorb to MPs, there is potential for MPs to alter co-contaminant bioavailability. Our objective was to demonstrate sorption of two co-contaminants with different physicochemistries [phenanthrene (Phe), log10Kow = 4.57; and 17α-ethinylestradiol (EE2), log10Kow = 3.67] to MPs; and assess whether co-contaminant bioavailability was increased after MP settlement. Bioavailability was indicated by gene expression in larval zebrafish. Both Phe and EE2 sorbed to MPs, which reduced bioavailability by a maximum of 33% and 48% respectively. Sorption occurred, but was not consistent with predictions based on co-contaminant physicochemistry (Phe having higher log10Kow was expected to have higher sorption). Contaminated MPs settled to the bottom of the exposures did not lead to increased bioavailability of Phe or EE2. Phe was 48% more bioavailable than predicted by a linear sorption model, organism-based measurements therefore contribute unique insight into MP co-contaminant bioavailability.",

keywords = "Danio rerio, microplastics, phenanthrene, 17α-ethinylestradiol, sorption, bioavailability",

author = "Sleight, {Victoria A.} and Adil Bakir and Thompson, {Richard C.} and Henry, {Theodore B.}",

note = "Acknowledgements Thank you to Stanley McMahon for fish maintenance and husbandry in the Zebrafish Research Facility, Plymouth, and Andrew Atfield and Dr. Helena Reinardy for laboratory assistance.",

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AU - Sleight, Victoria A.

AU - Bakir, Adil

AU - Thompson, Richard C.

AU - Henry, Theodore B.

N1 - Acknowledgements Thank you to Stanley McMahon for fish maintenance and husbandry in the Zebrafish Research Facility, Plymouth, and Andrew Atfield and Dr. Helena Reinardy for laboratory assistance.

PY - 2017/3/15

Y1 - 2017/3/15

N2 - Microplastics (MPs) are prevalent in marine ecosystems. Because toxicants (termed here “co-contaminants”) can sorb to MPs, there is potential for MPs to alter co-contaminant bioavailability. Our objective was to demonstrate sorption of two co-contaminants with different physicochemistries [phenanthrene (Phe), log10Kow = 4.57; and 17α-ethinylestradiol (EE2), log10Kow = 3.67] to MPs; and assess whether co-contaminant bioavailability was increased after MP settlement. Bioavailability was indicated by gene expression in larval zebrafish. Both Phe and EE2 sorbed to MPs, which reduced bioavailability by a maximum of 33% and 48% respectively. Sorption occurred, but was not consistent with predictions based on co-contaminant physicochemistry (Phe having higher log10Kow was expected to have higher sorption). Contaminated MPs settled to the bottom of the exposures did not lead to increased bioavailability of Phe or EE2. Phe was 48% more bioavailable than predicted by a linear sorption model, organism-based measurements therefore contribute unique insight into MP co-contaminant bioavailability.

AB - Microplastics (MPs) are prevalent in marine ecosystems. Because toxicants (termed here “co-contaminants”) can sorb to MPs, there is potential for MPs to alter co-contaminant bioavailability. Our objective was to demonstrate sorption of two co-contaminants with different physicochemistries [phenanthrene (Phe), log10Kow = 4.57; and 17α-ethinylestradiol (EE2), log10Kow = 3.67] to MPs; and assess whether co-contaminant bioavailability was increased after MP settlement. Bioavailability was indicated by gene expression in larval zebrafish. Both Phe and EE2 sorbed to MPs, which reduced bioavailability by a maximum of 33% and 48% respectively. Sorption occurred, but was not consistent with predictions based on co-contaminant physicochemistry (Phe having higher log10Kow was expected to have higher sorption). Contaminated MPs settled to the bottom of the exposures did not lead to increased bioavailability of Phe or EE2. Phe was 48% more bioavailable than predicted by a linear sorption model, organism-based measurements therefore contribute unique insight into MP co-contaminant bioavailability.

KW - Danio rerio

KW - microplastics

KW - phenanthrene

KW - 17α-ethinylestradiol

KW - sorption

KW - bioavailability

U2 - 10.1016/j.marpolbul.2016.12.055

DO - 10.1016/j.marpolbul.2016.12.055

M3 - Article

SN - 0025-326X

VL - 116

SP - 291

EP - 297

JO - Marine Pollution Bulletin

JF - Marine Pollution Bulletin

IS - 1-2

ER -

Assessment of microplastic-sorbed contaminant bioavailability through analysis of biomarker gene expression in larval zebrafish

Abstract

Bibliographical note

Keywords

UN SDGs

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Victoria Sleight

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