TY - CHAP
T1 - Assessment of the Teratogenic Effect of Drugs on the Chicken Embryo
AU - Rosa Fraga, Lucas
AU - de Oliveira, Maikel Rosa
AU - Wermann, Karina Maria
AU - Vargesson, Neil
N1 - Acknowledgements
Fraga’s Lab is funded by Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (FAPERGS), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Fundo de Incentivo à Pesquisa e Eventos (FIPE) of the Hospital de Clínicas de Porto Alegre (HCPA). The authors thank to Universidade Federal do Rio Grande do Sul (UFRGS) and HCPA.
PY - 2023/5/8
Y1 - 2023/5/8
N2 - Pre-clinical trials are an essential step that underpins the drug discovery, development and safety process. During this process, animal testing is performed to determine the safety of new compounds and any potential adverse effects. Developmental toxicity tests are carried out to verify whether the drug has potential to cause congenital anomalies to the developing embryo/foetus. Chicken embryos are very useful for these purposes and present several advantages, such as low cost of production and housing, easy handling and manipulation, rapid development in addition to sharing similarities to the human embryo at molecular, cellular, and anatomical levels. In this chapter, we bring methods for using the chicken embryo model for testing the teratogenic effects of drugs and assessing the main outcomes of them.
AB - Pre-clinical trials are an essential step that underpins the drug discovery, development and safety process. During this process, animal testing is performed to determine the safety of new compounds and any potential adverse effects. Developmental toxicity tests are carried out to verify whether the drug has potential to cause congenital anomalies to the developing embryo/foetus. Chicken embryos are very useful for these purposes and present several advantages, such as low cost of production and housing, easy handling and manipulation, rapid development in addition to sharing similarities to the human embryo at molecular, cellular, and anatomical levels. In this chapter, we bring methods for using the chicken embryo model for testing the teratogenic effects of drugs and assessing the main outcomes of them.
KW - Developmental toxicity
KW - Animal model
KW - Drug development
KW - Congenital anomalies
KW - Cartilage staining
KW - Morphology
M3 - Chapter
BT - Methods in Molecular Biology – Teratogenicity Testing Methods and Protocols
PB - Springer
ER -