Assessment of the toxicity of metals in soils amended with sewage sludge using a chemical speciation technique and a lux-based biosensor

S P McGrath, B Knight, K Killham, S Preston, G I Paton

Research output: Contribution to journalArticle

91 Citations (Scopus)

Abstract

Currently, regulations regarding the maximum permitted concentrations of metals in soils are based on measurements of the total concentration. However, a range of chemical and biological techniques are being developed to predict the bioavailable component of these pollutants. A lux-based biosensor was tested in soil solutions extracted from two field experiments at Braunschweig, Germany, that had the same metal inputs, but differed in pH. The bioluminescence response was found to decline as the free Zn2+ increased, and both soils fitted the same relationship with soil solution metal concentrations. The EC25 and EC50 derived from this curve were 1.9 and 6.1 mg/L, respectively. In contrast, the response to total Zn concentrations in the bulk soil showed distinct curves for each soil, further highlighting the appropriateness of free Zn2+ as a toxicity indicator. Other metals were present in the soil, but were unlikely to be toxic, because the observed concentrations were less than their individual toxic threshold values in solution. Bioluminescence-based biosensors were concluded to possibly offer an inexpensive and rapid technique to evaluate the bioavailability of metals in soil systems. The response of these biosensors can be related to soil solution speciation measurements, and this gives a common basis for expression of toxic thresholds in different soils.

Original languageEnglish
Pages (from-to)659-663
Number of pages5
JournalEnvironmental Toxicology and Chemistry
Volume18
Publication statusPublished - 1999

Keywords

  • pollution
  • zinc
  • copper
  • cadmium
  • nickel
  • LEGUMINOSARUM BIOVAR TRIFOLII
  • HEAVY-METALS
  • ZINC
  • CADMIUM
  • COPPER
  • CD
  • ZN
  • POPULATIONS
  • BIOMASS
  • ASSAY

Cite this

Assessment of the toxicity of metals in soils amended with sewage sludge using a chemical speciation technique and a lux-based biosensor. / McGrath, S P ; Knight, B ; Killham, K ; Preston, S ; Paton, G I .

In: Environmental Toxicology and Chemistry, Vol. 18, 1999, p. 659-663.

Research output: Contribution to journalArticle

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abstract = "Currently, regulations regarding the maximum permitted concentrations of metals in soils are based on measurements of the total concentration. However, a range of chemical and biological techniques are being developed to predict the bioavailable component of these pollutants. A lux-based biosensor was tested in soil solutions extracted from two field experiments at Braunschweig, Germany, that had the same metal inputs, but differed in pH. The bioluminescence response was found to decline as the free Zn2+ increased, and both soils fitted the same relationship with soil solution metal concentrations. The EC25 and EC50 derived from this curve were 1.9 and 6.1 mg/L, respectively. In contrast, the response to total Zn concentrations in the bulk soil showed distinct curves for each soil, further highlighting the appropriateness of free Zn2+ as a toxicity indicator. Other metals were present in the soil, but were unlikely to be toxic, because the observed concentrations were less than their individual toxic threshold values in solution. Bioluminescence-based biosensors were concluded to possibly offer an inexpensive and rapid technique to evaluate the bioavailability of metals in soil systems. The response of these biosensors can be related to soil solution speciation measurements, and this gives a common basis for expression of toxic thresholds in different soils.",
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AU - Knight, B

AU - Killham, K

AU - Preston, S

AU - Paton, G I

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AB - Currently, regulations regarding the maximum permitted concentrations of metals in soils are based on measurements of the total concentration. However, a range of chemical and biological techniques are being developed to predict the bioavailable component of these pollutants. A lux-based biosensor was tested in soil solutions extracted from two field experiments at Braunschweig, Germany, that had the same metal inputs, but differed in pH. The bioluminescence response was found to decline as the free Zn2+ increased, and both soils fitted the same relationship with soil solution metal concentrations. The EC25 and EC50 derived from this curve were 1.9 and 6.1 mg/L, respectively. In contrast, the response to total Zn concentrations in the bulk soil showed distinct curves for each soil, further highlighting the appropriateness of free Zn2+ as a toxicity indicator. Other metals were present in the soil, but were unlikely to be toxic, because the observed concentrations were less than their individual toxic threshold values in solution. Bioluminescence-based biosensors were concluded to possibly offer an inexpensive and rapid technique to evaluate the bioavailability of metals in soil systems. The response of these biosensors can be related to soil solution speciation measurements, and this gives a common basis for expression of toxic thresholds in different soils.

KW - pollution

KW - zinc

KW - copper

KW - cadmium

KW - nickel

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KW - ZINC

KW - CADMIUM

KW - COPPER

KW - CD

KW - ZN

KW - POPULATIONS

KW - BIOMASS

KW - ASSAY

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