Assignment of paternity groups without access to parental genotypes: multiple mating and developmental plasticity in squid

A. M. Emery, I. J. Wilson, S. Craig, Peter Robin Boyle, Leslie Robert Noble

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

We present a novel approach to investigating sibling relationships and reconstructing parental genotypes from a progeny array. The Bayesian method we have employed is flexible and may be applicable to a variety of situations in addition to the one presented here. While mutation rates and breeding population allele frequencies can be taken into account, the model requires relatively few loci and makes few assumptions. Paternity of 270 veined squid (Loligo forbesi) hatchlings from three egg strings collected from one location was assigned using five microsatellite loci. Paternal and maternal genotypes reconstructed for each of the three strings were identical, strongly indicating that a single female produced the strings that were fertilized by the same four males. The proportion of eggs fertilized was not equal between males in all three strings, with male 1 siring most offspring (up to 68% in string 1), through to male 4 siring the least (as low as 2.4% in string 1). Although temperature had a profound effect on incubation time, paternity did not affect this trait at 12 degreesC or 8 degreesC.

Original languageEnglish
Pages (from-to)1265-1278
Number of pages13
JournalMolecular Ecology
Volume10
Issue number5
DOIs
Publication statusPublished - May 2001

Keywords

  • Bayesian statistics
  • Loligo forbesi
  • microsatellite
  • parentage
  • paternity
  • squid
  • LOLIGO-FORBESI CEPHALOPODA
  • STATISTICAL CONFIDENCE
  • POPULATIONS
  • LOLIGINIDAE
  • MARKERS
  • LOCI
  • SCOTTISH
  • MUTATION
  • ALLELES
  • FEMALES

Cite this

Assignment of paternity groups without access to parental genotypes: multiple mating and developmental plasticity in squid. / Emery, A. M.; Wilson, I. J.; Craig, S.; Boyle, Peter Robin; Noble, Leslie Robert.

In: Molecular Ecology, Vol. 10, No. 5, 05.2001, p. 1265-1278.

Research output: Contribution to journalArticle

Emery, A. M. ; Wilson, I. J. ; Craig, S. ; Boyle, Peter Robin ; Noble, Leslie Robert. / Assignment of paternity groups without access to parental genotypes: multiple mating and developmental plasticity in squid. In: Molecular Ecology. 2001 ; Vol. 10, No. 5. pp. 1265-1278.
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