Association of heat shock protein70-2 (HSP70-2) gene polymorphism with coronary artery disease in an Iranian population

Maryam Mardan-Nik; Alireza Pasdar; Khadijeh Jamialahmadi; Atefeh Biabangard-Zak; Seyed Reza Mirhafez; Marzieh Ghalandari; Mohammad Tajfard; Mohsen Mohebati; Habibollah Esmaily; Gordon A. Ferns; Majid Ghayour-Mobarhan

doi:10.1016/j.gene.2014.08.012

Association of heat shock protein70-2 (HSP70-2) gene polymorphism with coronary artery disease in an Iranian population

Maryam Mardan-Nik, Alireza Pasdar, Khadijeh Jamialahmadi, Atefeh Biabangard-Zak, Seyed Reza Mirhafez, Marzieh Ghalandari, Mohammad Tajfard, Mohsen Mohebati, Habibollah Esmaily^*, Gordon A. Ferns, Majid Ghayour-Mobarhan

^*Corresponding author for this work

Applied Medicine

Research output: Contribution to journal › Article › peer-review

21 Citations (Scopus)

Abstract

Background: Coronary artery disease (CAD) is an inflammatory process and a major cause of mortality and morbidity. The (heat shock protein70-2) HSP70-2 gene is reported to be associated with coronary artery disease possibly by affecting the regulation of pro-inflammatory cytokines such as TNF-α. The association between CAD and the HSP70-2 gene +1267A>G polymorphism has been studied in some populations but there are no data about this association in the Iranian population. Aim: We have investigated the association between the HSP70-2 gene +1267A>G polymorphism and angiographically defined CAD within an Iranian population. Methods: We determined the presence of the HSP70-2 gene +1267A>G polymorphism in 628 patients with CAD and 307 healthy individuals using PCR-RFLP. Of the patients, 433 (68%) had >. 50% stenosis (CAD. +) and the remaining 195 patients had <. 50% stenosis (CAD. -), based on coronary angiography. Angiogram positive patients were subdivided into three groups: those with single (n. =. 113), double (n. =. 134), and triple vessels (n. =. 186) disease. Results: A significant higher frequency of AG. +. GG genotypes (G allele carriers) was observed in angiogram positive and angiogram negative groups compared to controls in a dominant analysis model of the HSP70-2 gene +1267A>G position (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). The allele frequency of the HSP70-2 G was also significantly higher in angiogram positive and angiogram negative groups compared to the control group (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). Conclusion: These results suggest that HSP70-2 +1267 polymorphism may influence the risk of CAD in Iranian population, however further studies are needed to clarify the role of other HSP70-2 gene polymorphisms in the pathogenesis of the CAD.

Original language	English
Pages (from-to)	180-184
Number of pages	5
Journal	Gene
Volume	550
Issue number	2
DOIs	https://doi.org/10.1016/j.gene.2014.08.012
Publication status	Published - 2014

Bibliographical note

Acknowledgments
The authors frankly thank all of the patients and their family members who volunteered to participate in this study. We are also particularly grateful to the Research Council of the Mashhad University of Medical Sciences (MUMS) for the financial support of the study (Grant No. 89370).

Keywords

Coronary artery disease
Heat shock protein70-2
HSP70-2 gene +1267A>G polymorphism
PCR-RFLP

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10.1016/j.gene.2014.08.012

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Mardan-Nik, M., Pasdar, A., Jamialahmadi, K., Biabangard-Zak, A., Mirhafez, S. R., Ghalandari, M., Tajfard, M., Mohebati, M., Esmaily, H., Ferns, G. A., & Ghayour-Mobarhan, M. (2014). Association of heat shock protein70-2 (HSP70-2) gene polymorphism with coronary artery disease in an Iranian population. Gene, 550(2), 180-184. https://doi.org/10.1016/j.gene.2014.08.012

@article{a4b222638aa04335a72d6b4502165821,

title = "Association of heat shock protein70-2 (HSP70-2) gene polymorphism with coronary artery disease in an Iranian population",

abstract = "Background: Coronary artery disease (CAD) is an inflammatory process and a major cause of mortality and morbidity. The (heat shock protein70-2) HSP70-2 gene is reported to be associated with coronary artery disease possibly by affecting the regulation of pro-inflammatory cytokines such as TNF-α. The association between CAD and the HSP70-2 gene +1267A>G polymorphism has been studied in some populations but there are no data about this association in the Iranian population. Aim: We have investigated the association between the HSP70-2 gene +1267A>G polymorphism and angiographically defined CAD within an Iranian population. Methods: We determined the presence of the HSP70-2 gene +1267A>G polymorphism in 628 patients with CAD and 307 healthy individuals using PCR-RFLP. Of the patients, 433 (68%) had >. 50% stenosis (CAD. +) and the remaining 195 patients had <. 50% stenosis (CAD. -), based on coronary angiography. Angiogram positive patients were subdivided into three groups: those with single (n. =. 113), double (n. =. 134), and triple vessels (n. =. 186) disease. Results: A significant higher frequency of AG. +. GG genotypes (G allele carriers) was observed in angiogram positive and angiogram negative groups compared to controls in a dominant analysis model of the HSP70-2 gene +1267A>G position (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). The allele frequency of the HSP70-2 G was also significantly higher in angiogram positive and angiogram negative groups compared to the control group (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). Conclusion: These results suggest that HSP70-2 +1267 polymorphism may influence the risk of CAD in Iranian population, however further studies are needed to clarify the role of other HSP70-2 gene polymorphisms in the pathogenesis of the CAD.",

keywords = "Coronary artery disease, Heat shock protein70-2, HSP70-2 gene +1267A>G polymorphism, PCR-RFLP",

author = "Maryam Mardan-Nik and Alireza Pasdar and Khadijeh Jamialahmadi and Atefeh Biabangard-Zak and Mirhafez, {Seyed Reza} and Marzieh Ghalandari and Mohammad Tajfard and Mohsen Mohebati and Habibollah Esmaily and Ferns, {Gordon A.} and Majid Ghayour-Mobarhan",

note = "Acknowledgments The authors frankly thank all of the patients and their family members who volunteered to participate in this study. We are also particularly grateful to the Research Council of the Mashhad University of Medical Sciences (MUMS) for the financial support of the study (Grant No. 89370).",

year = "2014",

doi = "10.1016/j.gene.2014.08.012",

language = "English",

volume = "550",

pages = "180--184",

journal = "Gene",

issn = "0378-1119",

publisher = "Elsevier",

number = "2",

}

TY - JOUR

T1 - Association of heat shock protein70-2 (HSP70-2) gene polymorphism with coronary artery disease in an Iranian population

AU - Mardan-Nik, Maryam

AU - Pasdar, Alireza

AU - Jamialahmadi, Khadijeh

AU - Biabangard-Zak, Atefeh

AU - Mirhafez, Seyed Reza

AU - Ghalandari, Marzieh

AU - Tajfard, Mohammad

AU - Mohebati, Mohsen

AU - Esmaily, Habibollah

AU - Ferns, Gordon A.

AU - Ghayour-Mobarhan, Majid

N1 - Acknowledgments The authors frankly thank all of the patients and their family members who volunteered to participate in this study. We are also particularly grateful to the Research Council of the Mashhad University of Medical Sciences (MUMS) for the financial support of the study (Grant No. 89370).

PY - 2014

Y1 - 2014

N2 - Background: Coronary artery disease (CAD) is an inflammatory process and a major cause of mortality and morbidity. The (heat shock protein70-2) HSP70-2 gene is reported to be associated with coronary artery disease possibly by affecting the regulation of pro-inflammatory cytokines such as TNF-α. The association between CAD and the HSP70-2 gene +1267A>G polymorphism has been studied in some populations but there are no data about this association in the Iranian population. Aim: We have investigated the association between the HSP70-2 gene +1267A>G polymorphism and angiographically defined CAD within an Iranian population. Methods: We determined the presence of the HSP70-2 gene +1267A>G polymorphism in 628 patients with CAD and 307 healthy individuals using PCR-RFLP. Of the patients, 433 (68%) had >. 50% stenosis (CAD. +) and the remaining 195 patients had <. 50% stenosis (CAD. -), based on coronary angiography. Angiogram positive patients were subdivided into three groups: those with single (n. =. 113), double (n. =. 134), and triple vessels (n. =. 186) disease. Results: A significant higher frequency of AG. +. GG genotypes (G allele carriers) was observed in angiogram positive and angiogram negative groups compared to controls in a dominant analysis model of the HSP70-2 gene +1267A>G position (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). The allele frequency of the HSP70-2 G was also significantly higher in angiogram positive and angiogram negative groups compared to the control group (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). Conclusion: These results suggest that HSP70-2 +1267 polymorphism may influence the risk of CAD in Iranian population, however further studies are needed to clarify the role of other HSP70-2 gene polymorphisms in the pathogenesis of the CAD.

AB - Background: Coronary artery disease (CAD) is an inflammatory process and a major cause of mortality and morbidity. The (heat shock protein70-2) HSP70-2 gene is reported to be associated with coronary artery disease possibly by affecting the regulation of pro-inflammatory cytokines such as TNF-α. The association between CAD and the HSP70-2 gene +1267A>G polymorphism has been studied in some populations but there are no data about this association in the Iranian population. Aim: We have investigated the association between the HSP70-2 gene +1267A>G polymorphism and angiographically defined CAD within an Iranian population. Methods: We determined the presence of the HSP70-2 gene +1267A>G polymorphism in 628 patients with CAD and 307 healthy individuals using PCR-RFLP. Of the patients, 433 (68%) had >. 50% stenosis (CAD. +) and the remaining 195 patients had <. 50% stenosis (CAD. -), based on coronary angiography. Angiogram positive patients were subdivided into three groups: those with single (n. =. 113), double (n. =. 134), and triple vessels (n. =. 186) disease. Results: A significant higher frequency of AG. +. GG genotypes (G allele carriers) was observed in angiogram positive and angiogram negative groups compared to controls in a dominant analysis model of the HSP70-2 gene +1267A>G position (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). The allele frequency of the HSP70-2 G was also significantly higher in angiogram positive and angiogram negative groups compared to the control group (51.2 vs. 43.2, P. =. 0.002, OR. =. 1.37) (51.0 vs. 43.2, P. =. 0.01, OR. =. 1.37). Conclusion: These results suggest that HSP70-2 +1267 polymorphism may influence the risk of CAD in Iranian population, however further studies are needed to clarify the role of other HSP70-2 gene polymorphisms in the pathogenesis of the CAD.

KW - Coronary artery disease

KW - Heat shock protein70-2

KW - HSP70-2 gene +1267A>G polymorphism

KW - PCR-RFLP

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U2 - 10.1016/j.gene.2014.08.012

DO - 10.1016/j.gene.2014.08.012

M3 - Article

C2 - 25108126

AN - SCOPUS:84921681435

SN - 0378-1119

VL - 550

SP - 180

EP - 184

JO - Gene

JF - Gene

IS - 2

ER -

Association of heat shock protein70-2 (HSP70-2) gene polymorphism with coronary artery disease in an Iranian population

Abstract

Bibliographical note

Keywords

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