Association of leptin receptor (OB-Rb), NPY and GLP-1 gene expression in the ovine and murine brainstem

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Abstract

Appetite-related neuropeptide systems have not been studied extensively in the ruminant, although there have been a number of recent studies of the hypothalamus. Since some leptin signaling is integrated in the rodent brainstem, and leptin modulates neuropeptidergic activity, we now describe leptin receptor (long splice variant, OB-Rb), neuropeptide Y (NPY) and glucagon-like peptide-1 (GLP-1) gene expression in the ovine brainstem. Leptin receptor mRNA was localized to the spinal trigeminal tract and nucleus, nucleus of the solitary tract (NTS), area postrema and dorsal motor nucleus of the vagus. NPY gene expression was abundant in the ovine medulla, occurring in two bilateral 'bands' that encompassed the NTS region and ran ventrolaterally. GLP-1 mRNA was confined largely to the NTS. The distribution of OB-Rb mRNA overlapped with that of NPY and GLP-1 gene expression, suggesting the possibility of interaction between leptin and these brainstem neuropeptide systems. However, in an extension of earlier work, co-expression studies in the murine brainstem revealed only a small number of neurons that expressed both NPY and leptin receptor mRNA, despite the widespread and abundant expression of the former. Thus the majority of NPY synthesis in the brainstem may not be directly regulated by leptin. The sheep brainstem had similar anatomical distribution of OB-Rb, NPY and GLP-1 gene expression to the rodent, consistent with a role for this region in peripheral leptin feedback signaling and brainstem-hypothalamo communication. (C) 1998 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)271-278
Number of pages8
JournalRegulatory Peptides
Volume75-76
DOIs
Publication statusPublished - 25 Sep 1998

Keywords

  • sheep
  • hindbrain
  • area postrema
  • nucleus of the solitary tract
  • dorsal motor nucleus of the vagus
  • GLUCAGON-LIKE PEPTIDE-1
  • HYPOTHALAMIC NEUROPEPTIDE-Y
  • FORM SPLICE VARIANT
  • MESSENGER-RNA
  • LUTEINIZING-HORMONE
  • OBESE GENE
  • MOUSE HYPOTHALAMUS
  • FOOD RESTRICTION
  • RAT HYPOTHALAMUS
  • BODY-WEIGHT
  • dorsal motor nucleus of the vagus
  • glucagon-like peptide-1
  • hypothalmic neropeptide-Y
  • form splice variant
  • messenger-RNA
  • luteinizing-hormone
  • obese gene
  • mouse hypothalamus
  • food restriction
  • rat hypothalamus
  • body-weight

Cite this

@article{d44fdaa82c9447f090b1b3dd4a70afb3,
title = "Association of leptin receptor (OB-Rb), NPY and GLP-1 gene expression in the ovine and murine brainstem",
abstract = "Appetite-related neuropeptide systems have not been studied extensively in the ruminant, although there have been a number of recent studies of the hypothalamus. Since some leptin signaling is integrated in the rodent brainstem, and leptin modulates neuropeptidergic activity, we now describe leptin receptor (long splice variant, OB-Rb), neuropeptide Y (NPY) and glucagon-like peptide-1 (GLP-1) gene expression in the ovine brainstem. Leptin receptor mRNA was localized to the spinal trigeminal tract and nucleus, nucleus of the solitary tract (NTS), area postrema and dorsal motor nucleus of the vagus. NPY gene expression was abundant in the ovine medulla, occurring in two bilateral 'bands' that encompassed the NTS region and ran ventrolaterally. GLP-1 mRNA was confined largely to the NTS. The distribution of OB-Rb mRNA overlapped with that of NPY and GLP-1 gene expression, suggesting the possibility of interaction between leptin and these brainstem neuropeptide systems. However, in an extension of earlier work, co-expression studies in the murine brainstem revealed only a small number of neurons that expressed both NPY and leptin receptor mRNA, despite the widespread and abundant expression of the former. Thus the majority of NPY synthesis in the brainstem may not be directly regulated by leptin. The sheep brainstem had similar anatomical distribution of OB-Rb, NPY and GLP-1 gene expression to the rodent, consistent with a role for this region in peripheral leptin feedback signaling and brainstem-hypothalamo communication. (C) 1998 Elsevier Science B.V. All rights reserved.",
keywords = "sheep, hindbrain, area postrema, nucleus of the solitary tract, dorsal motor nucleus of the vagus, GLUCAGON-LIKE PEPTIDE-1, HYPOTHALAMIC NEUROPEPTIDE-Y, FORM SPLICE VARIANT, MESSENGER-RNA, LUTEINIZING-HORMONE, OBESE GENE, MOUSE HYPOTHALAMUS, FOOD RESTRICTION, RAT HYPOTHALAMUS, BODY-WEIGHT, dorsal motor nucleus of the vagus , glucagon-like peptide-1, hypothalmic neropeptide-Y, form splice variant, messenger-RNA, luteinizing-hormone, obese gene, mouse hypothalamus, food restriction, rat hypothalamus, body-weight",
author = "Julian Mercer and Kim-Marie Moar and P.A. Findlay and Nigel Hoggard and Adam, {Clare Lesley}",
year = "1998",
month = "9",
day = "25",
doi = "10.1016/S0167-0115(98)00078-0",
language = "English",
volume = "75-76",
pages = "271--278",
journal = "Regulatory Peptides",
issn = "0167-0115",
publisher = "Elsevier",

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TY - JOUR

T1 - Association of leptin receptor (OB-Rb), NPY and GLP-1 gene expression in the ovine and murine brainstem

AU - Mercer, Julian

AU - Moar, Kim-Marie

AU - Findlay, P.A.

AU - Hoggard, Nigel

AU - Adam, Clare Lesley

PY - 1998/9/25

Y1 - 1998/9/25

N2 - Appetite-related neuropeptide systems have not been studied extensively in the ruminant, although there have been a number of recent studies of the hypothalamus. Since some leptin signaling is integrated in the rodent brainstem, and leptin modulates neuropeptidergic activity, we now describe leptin receptor (long splice variant, OB-Rb), neuropeptide Y (NPY) and glucagon-like peptide-1 (GLP-1) gene expression in the ovine brainstem. Leptin receptor mRNA was localized to the spinal trigeminal tract and nucleus, nucleus of the solitary tract (NTS), area postrema and dorsal motor nucleus of the vagus. NPY gene expression was abundant in the ovine medulla, occurring in two bilateral 'bands' that encompassed the NTS region and ran ventrolaterally. GLP-1 mRNA was confined largely to the NTS. The distribution of OB-Rb mRNA overlapped with that of NPY and GLP-1 gene expression, suggesting the possibility of interaction between leptin and these brainstem neuropeptide systems. However, in an extension of earlier work, co-expression studies in the murine brainstem revealed only a small number of neurons that expressed both NPY and leptin receptor mRNA, despite the widespread and abundant expression of the former. Thus the majority of NPY synthesis in the brainstem may not be directly regulated by leptin. The sheep brainstem had similar anatomical distribution of OB-Rb, NPY and GLP-1 gene expression to the rodent, consistent with a role for this region in peripheral leptin feedback signaling and brainstem-hypothalamo communication. (C) 1998 Elsevier Science B.V. All rights reserved.

AB - Appetite-related neuropeptide systems have not been studied extensively in the ruminant, although there have been a number of recent studies of the hypothalamus. Since some leptin signaling is integrated in the rodent brainstem, and leptin modulates neuropeptidergic activity, we now describe leptin receptor (long splice variant, OB-Rb), neuropeptide Y (NPY) and glucagon-like peptide-1 (GLP-1) gene expression in the ovine brainstem. Leptin receptor mRNA was localized to the spinal trigeminal tract and nucleus, nucleus of the solitary tract (NTS), area postrema and dorsal motor nucleus of the vagus. NPY gene expression was abundant in the ovine medulla, occurring in two bilateral 'bands' that encompassed the NTS region and ran ventrolaterally. GLP-1 mRNA was confined largely to the NTS. The distribution of OB-Rb mRNA overlapped with that of NPY and GLP-1 gene expression, suggesting the possibility of interaction between leptin and these brainstem neuropeptide systems. However, in an extension of earlier work, co-expression studies in the murine brainstem revealed only a small number of neurons that expressed both NPY and leptin receptor mRNA, despite the widespread and abundant expression of the former. Thus the majority of NPY synthesis in the brainstem may not be directly regulated by leptin. The sheep brainstem had similar anatomical distribution of OB-Rb, NPY and GLP-1 gene expression to the rodent, consistent with a role for this region in peripheral leptin feedback signaling and brainstem-hypothalamo communication. (C) 1998 Elsevier Science B.V. All rights reserved.

KW - sheep

KW - hindbrain

KW - area postrema

KW - nucleus of the solitary tract

KW - dorsal motor nucleus of the vagus

KW - GLUCAGON-LIKE PEPTIDE-1

KW - HYPOTHALAMIC NEUROPEPTIDE-Y

KW - FORM SPLICE VARIANT

KW - MESSENGER-RNA

KW - LUTEINIZING-HORMONE

KW - OBESE GENE

KW - MOUSE HYPOTHALAMUS

KW - FOOD RESTRICTION

KW - RAT HYPOTHALAMUS

KW - BODY-WEIGHT

KW - dorsal motor nucleus of the vagus

KW - glucagon-like peptide-1

KW - hypothalmic neropeptide-Y

KW - form splice variant

KW - messenger-RNA

KW - luteinizing-hormone

KW - obese gene

KW - mouse hypothalamus

KW - food restriction

KW - rat hypothalamus

KW - body-weight

U2 - 10.1016/S0167-0115(98)00078-0

DO - 10.1016/S0167-0115(98)00078-0

M3 - Article

VL - 75-76

SP - 271

EP - 278

JO - Regulatory Peptides

JF - Regulatory Peptides

SN - 0167-0115

ER -