TY - JOUR
T1 - Association of PICK1 and BDNF variations with increased risk of methamphetamine dependence among Iranian population
T2 - a case–control study
AU - Tajbakhsh, Amir
AU - Alimardani, Maliheh
AU - Asghari, Mahla
AU - Abedini, Soheila
AU - Saghafi Khadem, Sohrab
AU - Nesaei Bajestani, Abolfazl
AU - Alipoor, Forough
AU - Alidoust, Maryam
AU - Savardashtaki, Amir
AU - Hashemian, Peyman
AU - Pasdar, Alireza
N1 - Funding Information:
This study was financially supported by the Mashhad University of Medical Sciences (Grant Number: 931681).
PY - 2021/1/26
Y1 - 2021/1/26
N2 - Background: Genetic factors play an important role in susceptibility to methamphetamine dependency. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a case–control study, we investigated the association between polymorphisms of PICK1 and BDNF genes and methamphetamine dependence in an Iranian population. Methods: Total of 235 cases and 204 controls were recruited in a period between 2015 to 2018. The PICK1-rs713729, -rs2076369 and BDNF-rs6265 genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0. Results: In the present study, two polymorphisms including PICK1-rs713729 (OR 1.38 (CI 1.08–1.52; P-value 0.004) in multiplicative and dominant models, and PICK1-rs2076369 (OR 1.31 (CI 1.10–1.56; P-value 0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis showed a significant association of haplotype AG (OR 2.50 (CI 1.50–4.16; P-value 0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR 0.67 (CI 0.50–0.91; P-value 0.009) in dominant and co-dominant models with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium. Conclusion: Our findings indicate that the PICK1 gene polymorphism might affect the risk of methamphetamine dependency in our population.
AB - Background: Genetic factors play an important role in susceptibility to methamphetamine dependency. In this line, protein that interact with C-kinase-1 (PICK1) and brain-derived neurotrophic factor (BDNF) genes are linked to methamphetamine dependence (substance use disorder). Thus, in a case–control study, we investigated the association between polymorphisms of PICK1 and BDNF genes and methamphetamine dependence in an Iranian population. Methods: Total of 235 cases and 204 controls were recruited in a period between 2015 to 2018. The PICK1-rs713729, -rs2076369 and BDNF-rs6265 genotypes were determined via ARMS-PCR assay. Statistical analysis was performed, using SPSS 20.0, PHASE 2.1.1 program as well as SNP Analyzer 2.0. Results: In the present study, two polymorphisms including PICK1-rs713729 (OR 1.38 (CI 1.08–1.52; P-value 0.004) in multiplicative and dominant models, and PICK1-rs2076369 (OR 1.31 (CI 1.10–1.56; P-value 0.002) in multiplicative, dominant and co-dominant models were associated with the risk of methamphetamine abuse. Moreover, haplotype analysis showed a significant association of haplotype AG (OR 2.50 (CI 1.50–4.16; P-value 0.0002) in dominant, recessive and co-dominant models, and haplotype TT (OR 0.67 (CI 0.50–0.91; P-value 0.009) in dominant and co-dominant models with the risk of methamphetamine abuse. None of the polymorphisms in this study had a high level of linkage disequilibrium. Conclusion: Our findings indicate that the PICK1 gene polymorphism might affect the risk of methamphetamine dependency in our population.
KW - Addiction
KW - And polymorphisms
KW - Dopamine pathway
KW - Drug abuse
KW - Glutamate pathway
KW - Substance dependence
KW - Substance use disorder (SUD)
KW - Variations
UR - http://www.scopus.com/inward/record.url?scp=85099831888&partnerID=8YFLogxK
U2 - 10.1186/s12920-021-00873-7
DO - 10.1186/s12920-021-00873-7
M3 - Article
C2 - 33499851
AN - SCOPUS:85099831888
SN - 1755-8794
VL - 14
JO - BMC Medical Genomics
JF - BMC Medical Genomics
IS - 1
M1 - 27
ER -