Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV)

Jose Garcia, Eann S Munro, Milena M Monte, Mickael C S Fourrier, Jason Whitelaw, David A Smail, Anthony E Ellis

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Vitellogenin is a phosphoglycoprotein which represents the main precursor of the egg yolk in teleost fish. This reproductive protein was also demonstrated to play an important role in innate immunity by acting as a pattern recognition molecule capable of binding to bacteria, fungi and enhancing macrophage phagocytosis. The presented results demonstrate that, egg homogenate, ovarian fluid and serum of mature female Atlantic salmon have high neutralising ability for infectious pancreatic necrosis virus (IPNV). Vitellogenin from mature female Atlantic salmon serum, purified by immuno-affinity on a column matrix coated with monoclonal anti-Atlantic salmon vitellogenin antibody, was able to neutralise between 9.1 × 104 and 3.09 × 105 TCID50 IPNV mg−1 of protein. To the author’s knowledge, this is the first time that the neutralising activity of vitellogenin on a teleost virus has been demonstrated. The results may explain why IPNV is difficult to detect by culture methods in ovarian fluid and egg homogenates from carrier mature females and suggest a possible means of vertical transmission via the egg.
Original languageEnglish
Pages (from-to)293-297
Number of pages5
JournalFish & Shellfish Immunology
Volume29
Issue number2
DOIs
Publication statusPublished - Aug 2010

Fingerprint

Infectious pancreatic necrosis virus
Vitellogenins
infectivity
vitellogenin
Viruses
Salmo salar
serum
virus
pathogenicity
egg
teleost
neutralization
vertical transmission
protein
Fluids
fluid
Macrophages
pattern recognition
immunity
Fungi

Cite this

Garcia, J., Munro, E. S., Monte, M. M., Fourrier, M. C. S., Whitelaw, J., Smail, D. A., & Ellis, A. E. (2010). Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV). Fish & Shellfish Immunology, 29(2), 293-297. https://doi.org/10.1016/j.fsi.2010.04.010

Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV). / Garcia, Jose; Munro, Eann S; Monte, Milena M; Fourrier, Mickael C S; Whitelaw, Jason; Smail, David A; Ellis, Anthony E.

In: Fish & Shellfish Immunology, Vol. 29, No. 2, 08.2010, p. 293-297.

Research output: Contribution to journalArticle

Garcia, Jose ; Munro, Eann S ; Monte, Milena M ; Fourrier, Mickael C S ; Whitelaw, Jason ; Smail, David A ; Ellis, Anthony E. / Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV). In: Fish & Shellfish Immunology. 2010 ; Vol. 29, No. 2. pp. 293-297.
@article{c9110e502af64fac994b77862cbb56f2,
title = "Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV)",
abstract = "Vitellogenin is a phosphoglycoprotein which represents the main precursor of the egg yolk in teleost fish. This reproductive protein was also demonstrated to play an important role in innate immunity by acting as a pattern recognition molecule capable of binding to bacteria, fungi and enhancing macrophage phagocytosis. The presented results demonstrate that, egg homogenate, ovarian fluid and serum of mature female Atlantic salmon have high neutralising ability for infectious pancreatic necrosis virus (IPNV). Vitellogenin from mature female Atlantic salmon serum, purified by immuno-affinity on a column matrix coated with monoclonal anti-Atlantic salmon vitellogenin antibody, was able to neutralise between 9.1 × 104 and 3.09 × 105 TCID50 IPNV mg−1 of protein. To the author’s knowledge, this is the first time that the neutralising activity of vitellogenin on a teleost virus has been demonstrated. The results may explain why IPNV is difficult to detect by culture methods in ovarian fluid and egg homogenates from carrier mature females and suggest a possible means of vertical transmission via the egg.",
author = "Jose Garcia and Munro, {Eann S} and Monte, {Milena M} and Fourrier, {Mickael C S} and Jason Whitelaw and Smail, {David A} and Ellis, {Anthony E}",
year = "2010",
month = "8",
doi = "10.1016/j.fsi.2010.04.010",
language = "English",
volume = "29",
pages = "293--297",
journal = "Fish & Shellfish Immunology",
issn = "1050-4648",
publisher = "ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD",
number = "2",

}

TY - JOUR

T1 - Atlantic salmon (Salmo salar L.) serum vitellogenin neutralises infectivity of infectious pancreatic necrosis virus (IPNV)

AU - Garcia, Jose

AU - Munro, Eann S

AU - Monte, Milena M

AU - Fourrier, Mickael C S

AU - Whitelaw, Jason

AU - Smail, David A

AU - Ellis, Anthony E

PY - 2010/8

Y1 - 2010/8

N2 - Vitellogenin is a phosphoglycoprotein which represents the main precursor of the egg yolk in teleost fish. This reproductive protein was also demonstrated to play an important role in innate immunity by acting as a pattern recognition molecule capable of binding to bacteria, fungi and enhancing macrophage phagocytosis. The presented results demonstrate that, egg homogenate, ovarian fluid and serum of mature female Atlantic salmon have high neutralising ability for infectious pancreatic necrosis virus (IPNV). Vitellogenin from mature female Atlantic salmon serum, purified by immuno-affinity on a column matrix coated with monoclonal anti-Atlantic salmon vitellogenin antibody, was able to neutralise between 9.1 × 104 and 3.09 × 105 TCID50 IPNV mg−1 of protein. To the author’s knowledge, this is the first time that the neutralising activity of vitellogenin on a teleost virus has been demonstrated. The results may explain why IPNV is difficult to detect by culture methods in ovarian fluid and egg homogenates from carrier mature females and suggest a possible means of vertical transmission via the egg.

AB - Vitellogenin is a phosphoglycoprotein which represents the main precursor of the egg yolk in teleost fish. This reproductive protein was also demonstrated to play an important role in innate immunity by acting as a pattern recognition molecule capable of binding to bacteria, fungi and enhancing macrophage phagocytosis. The presented results demonstrate that, egg homogenate, ovarian fluid and serum of mature female Atlantic salmon have high neutralising ability for infectious pancreatic necrosis virus (IPNV). Vitellogenin from mature female Atlantic salmon serum, purified by immuno-affinity on a column matrix coated with monoclonal anti-Atlantic salmon vitellogenin antibody, was able to neutralise between 9.1 × 104 and 3.09 × 105 TCID50 IPNV mg−1 of protein. To the author’s knowledge, this is the first time that the neutralising activity of vitellogenin on a teleost virus has been demonstrated. The results may explain why IPNV is difficult to detect by culture methods in ovarian fluid and egg homogenates from carrier mature females and suggest a possible means of vertical transmission via the egg.

U2 - 10.1016/j.fsi.2010.04.010

DO - 10.1016/j.fsi.2010.04.010

M3 - Article

VL - 29

SP - 293

EP - 297

JO - Fish & Shellfish Immunology

JF - Fish & Shellfish Immunology

SN - 1050-4648

IS - 2

ER -