ATRIAL-NATRIURETIC-FACTOR MODULATES NITRIC-OXIDE PRODUCTION - AN ANF-C RECEPTOR-MEDIATED EFFECT

J S MCLAY, P K CHATTERJEE, A G JARDINE, G M HAWKSWORTH

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

Objectives: To investigate the possible immunomodulatory and regulatory functions of atrial natriuretic factor (ANF) and the natriuretic peptide C (NPR-C) receptor in the control of cytokine-stimulated nitric oxide production in primary cultures of human proximal tubular cells.

Methods: Freshly prepared human proximal tubular cells were seeded on plastic plates and allowed to reach confluence. The confluent cells were then incubated with ANF or cyclic((4-23))ANF (C-(4-23)ANF) alone, or preincubated with ANF or C-(4-23)ANF before incubation with the nitric oxide-stimulating combination of cytokines interleukin-1 beta (10 u/ml), tumour necrosis factor-alpha (10 ng/ml) and interferon-gamma (100 u/ml).

Results: In the present series of experiments we have found that incubation of primary cultures of human proximal tubular cells with ANF or C-(4-23)ANF stimulates nitric oxide production dose-dependently, Paradoxically, ANF acting via the NPR-C receptor also inhibits cytokine activation of the enzyme-inducible nitric oxide synthase via a cyclic GMP-independent mechanism. Both of these effects were reproduced by the NPR-C receptor-specific ligand C-(4-23)ANF.

Conclusions: These findings represent novel actions of ANF mediated via the NPR-C receptor. The results also provide a simple model system in which to study the subcellular mechanisms of NPR-C receptor activation.

Original languageEnglish
Pages (from-to)625-630
Number of pages6
JournalJournal of Hypertension
Volume13
Issue number6
Publication statusPublished - Jun 1995

Keywords

  • ANF
  • ANF-C RECEPTOR
  • NITRIC OXIDE
  • IMMUNOMODULATION
  • CYCLIC GMP
  • CYTOKINE
  • ENDOTHELIAL-CELLS
  • SMOOTH-MUSCLE
  • PEPTIDE
  • ACETYLCHOLINE
  • MECHANISMS

Cite this

ATRIAL-NATRIURETIC-FACTOR MODULATES NITRIC-OXIDE PRODUCTION - AN ANF-C RECEPTOR-MEDIATED EFFECT. / MCLAY, J S ; CHATTERJEE, P K ; JARDINE, A G ; HAWKSWORTH, G M .

In: Journal of Hypertension, Vol. 13, No. 6, 06.1995, p. 625-630.

Research output: Contribution to journalArticle

MCLAY, JS, CHATTERJEE, PK, JARDINE, AG & HAWKSWORTH, GM 1995, 'ATRIAL-NATRIURETIC-FACTOR MODULATES NITRIC-OXIDE PRODUCTION - AN ANF-C RECEPTOR-MEDIATED EFFECT', Journal of Hypertension, vol. 13, no. 6, pp. 625-630.
MCLAY, J S ; CHATTERJEE, P K ; JARDINE, A G ; HAWKSWORTH, G M . / ATRIAL-NATRIURETIC-FACTOR MODULATES NITRIC-OXIDE PRODUCTION - AN ANF-C RECEPTOR-MEDIATED EFFECT. In: Journal of Hypertension. 1995 ; Vol. 13, No. 6. pp. 625-630.
@article{111929c3bd934a3885d51e332e17bc08,
title = "ATRIAL-NATRIURETIC-FACTOR MODULATES NITRIC-OXIDE PRODUCTION - AN ANF-C RECEPTOR-MEDIATED EFFECT",
abstract = "Objectives: To investigate the possible immunomodulatory and regulatory functions of atrial natriuretic factor (ANF) and the natriuretic peptide C (NPR-C) receptor in the control of cytokine-stimulated nitric oxide production in primary cultures of human proximal tubular cells.Methods: Freshly prepared human proximal tubular cells were seeded on plastic plates and allowed to reach confluence. The confluent cells were then incubated with ANF or cyclic((4-23))ANF (C-(4-23)ANF) alone, or preincubated with ANF or C-(4-23)ANF before incubation with the nitric oxide-stimulating combination of cytokines interleukin-1 beta (10 u/ml), tumour necrosis factor-alpha (10 ng/ml) and interferon-gamma (100 u/ml).Results: In the present series of experiments we have found that incubation of primary cultures of human proximal tubular cells with ANF or C-(4-23)ANF stimulates nitric oxide production dose-dependently, Paradoxically, ANF acting via the NPR-C receptor also inhibits cytokine activation of the enzyme-inducible nitric oxide synthase via a cyclic GMP-independent mechanism. Both of these effects were reproduced by the NPR-C receptor-specific ligand C-(4-23)ANF.Conclusions: These findings represent novel actions of ANF mediated via the NPR-C receptor. The results also provide a simple model system in which to study the subcellular mechanisms of NPR-C receptor activation.",
keywords = "ANF, ANF-C RECEPTOR, NITRIC OXIDE, IMMUNOMODULATION, CYCLIC GMP, CYTOKINE, ENDOTHELIAL-CELLS, SMOOTH-MUSCLE, PEPTIDE, ACETYLCHOLINE, MECHANISMS",
author = "MCLAY, {J S} and CHATTERJEE, {P K} and JARDINE, {A G} and HAWKSWORTH, {G M}",
year = "1995",
month = "6",
language = "English",
volume = "13",
pages = "625--630",
journal = "Journal of Hypertension",
issn = "0263-6352",
publisher = "Lippincott Williams and Wilkins",
number = "6",

}

TY - JOUR

T1 - ATRIAL-NATRIURETIC-FACTOR MODULATES NITRIC-OXIDE PRODUCTION - AN ANF-C RECEPTOR-MEDIATED EFFECT

AU - MCLAY, J S

AU - CHATTERJEE, P K

AU - JARDINE, A G

AU - HAWKSWORTH, G M

PY - 1995/6

Y1 - 1995/6

N2 - Objectives: To investigate the possible immunomodulatory and regulatory functions of atrial natriuretic factor (ANF) and the natriuretic peptide C (NPR-C) receptor in the control of cytokine-stimulated nitric oxide production in primary cultures of human proximal tubular cells.Methods: Freshly prepared human proximal tubular cells were seeded on plastic plates and allowed to reach confluence. The confluent cells were then incubated with ANF or cyclic((4-23))ANF (C-(4-23)ANF) alone, or preincubated with ANF or C-(4-23)ANF before incubation with the nitric oxide-stimulating combination of cytokines interleukin-1 beta (10 u/ml), tumour necrosis factor-alpha (10 ng/ml) and interferon-gamma (100 u/ml).Results: In the present series of experiments we have found that incubation of primary cultures of human proximal tubular cells with ANF or C-(4-23)ANF stimulates nitric oxide production dose-dependently, Paradoxically, ANF acting via the NPR-C receptor also inhibits cytokine activation of the enzyme-inducible nitric oxide synthase via a cyclic GMP-independent mechanism. Both of these effects were reproduced by the NPR-C receptor-specific ligand C-(4-23)ANF.Conclusions: These findings represent novel actions of ANF mediated via the NPR-C receptor. The results also provide a simple model system in which to study the subcellular mechanisms of NPR-C receptor activation.

AB - Objectives: To investigate the possible immunomodulatory and regulatory functions of atrial natriuretic factor (ANF) and the natriuretic peptide C (NPR-C) receptor in the control of cytokine-stimulated nitric oxide production in primary cultures of human proximal tubular cells.Methods: Freshly prepared human proximal tubular cells were seeded on plastic plates and allowed to reach confluence. The confluent cells were then incubated with ANF or cyclic((4-23))ANF (C-(4-23)ANF) alone, or preincubated with ANF or C-(4-23)ANF before incubation with the nitric oxide-stimulating combination of cytokines interleukin-1 beta (10 u/ml), tumour necrosis factor-alpha (10 ng/ml) and interferon-gamma (100 u/ml).Results: In the present series of experiments we have found that incubation of primary cultures of human proximal tubular cells with ANF or C-(4-23)ANF stimulates nitric oxide production dose-dependently, Paradoxically, ANF acting via the NPR-C receptor also inhibits cytokine activation of the enzyme-inducible nitric oxide synthase via a cyclic GMP-independent mechanism. Both of these effects were reproduced by the NPR-C receptor-specific ligand C-(4-23)ANF.Conclusions: These findings represent novel actions of ANF mediated via the NPR-C receptor. The results also provide a simple model system in which to study the subcellular mechanisms of NPR-C receptor activation.

KW - ANF

KW - ANF-C RECEPTOR

KW - NITRIC OXIDE

KW - IMMUNOMODULATION

KW - CYCLIC GMP

KW - CYTOKINE

KW - ENDOTHELIAL-CELLS

KW - SMOOTH-MUSCLE

KW - PEPTIDE

KW - ACETYLCHOLINE

KW - MECHANISMS

M3 - Article

VL - 13

SP - 625

EP - 630

JO - Journal of Hypertension

JF - Journal of Hypertension

SN - 0263-6352

IS - 6

ER -