Australian plants with potential to inhibit bacteria and processes involved in ruminal biohydrogenation of fatty acids

Z. Durmic, C. S. McSweeney, G. W. Kemp, P. Hutton, R. J. Wallace, P. E. Vercoe

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Conjugated linoleic acids (CLA) are health-promoting fatty acids found in foods derived from ruminant products that are formed in the rumen during bacterial biohydrogenation of linoleic acid (LA). Although selective antimicrobial,, might increase CLA production by manipulation of ruminal microflora, feeding of antibiotic growth promoters to livestock is declining due to fears of development of antibiotic resistance in human pathogens. This has initiated a search for alternatives, including plants containing bioactive compounds, which can have similar positive effects on ruminal microflora, but without negative impact on animal and human health. In this study we investigated effects of natural compounds in Australian plants on the bacteria and processes involved in ruminal biohydrogenation. Ethanolic extracts and essential oils were obtained from 91 Australian plants collected during 2004/2005 in Western Australia. Minimal Inhibitory Concentrations for the main bacterial species involved in rumen biohydrogenation were established using an agar dilution method, and effects of selected candidates off LA biohydrogenation was examined using an in vitro system with a mixed rumen bacterial population. A wide range of the plant extracts had selective inhibitory effect towards Clostridium proteoclasticum (which forms stearate from LA) without affecting Butyrivibrio fibrosolvens (which forms CLA and vaccenic acid, but not stearate). However, only a few plants, including Acacia iteaphylla and Kennedia eximia, inhibited LA metabolism or stearate formation in the mixed bacterial community in vitro. Further experiments are needed to investigate effects of selected candidates on CLA production in vivo. Crown Copyright (C) 2007 Published by Elsevier B.V. All rights reserved.

Original languageEnglish
Pages (from-to)271-284
Number of pages14
JournalAnimal Feed Science and Technology
Volume145
Issue number1-4
Early online date13 Aug 2007
DOIs
Publication statusPublished - 14 Aug 2008

Keywords

  • conjugated linoleic acids
  • rumen bacteria
  • rumen hydrogenation
  • plant extract
  • rumen microbial fermentation
  • essential oils
  • in-vitro
  • antimicrobial agents
  • continuous-culture
  • medicinal-plants
  • extracts
  • metabolism
  • hydrogenation

Cite this

Australian plants with potential to inhibit bacteria and processes involved in ruminal biohydrogenation of fatty acids. / Durmic, Z.; McSweeney, C. S.; Kemp, G. W.; Hutton, P.; Wallace, R. J.; Vercoe, P. E.

In: Animal Feed Science and Technology, Vol. 145, No. 1-4, 14.08.2008, p. 271-284.

Research output: Contribution to journalArticle

Durmic, Z. ; McSweeney, C. S. ; Kemp, G. W. ; Hutton, P. ; Wallace, R. J. ; Vercoe, P. E. / Australian plants with potential to inhibit bacteria and processes involved in ruminal biohydrogenation of fatty acids. In: Animal Feed Science and Technology. 2008 ; Vol. 145, No. 1-4. pp. 271-284.
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