B219/OB-R 5'-UTR and leptin receptor gene-related protein gene expression in mouse brain and placenta tissue-specific leptin receptor promoter activity

Julian Mercer, Kim-Marie Moar, Nigel Hoggard, A D Strosberg, P Froguel, B Bailleul

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Abstract

Leptin receptor (OB-R) splice variants either encode proteins with different 3' cytoplasmic domains or have different 5' untranslated regions (UTR), indicative of dual promoters. The B219/OB-R promoter transcribes only OB-R transcripts, whereas the OB-R/GRP promoter initiates transcription of both OB-R and another protein of unknown function, called the leptin receptor gene-related protein (OB-RGRP). We compared expression of B219/OB-R 5'-UTR and OB-RGRP mRNAs by in situ hybridization. We thus assessed, by inference, the contributions of the two promoters to the leptin receptor transcript pool, in murine brain or in placenta, a tissue with abundant leptin receptor mRNA. Expression of B219/OB-R 5'-UTR mRNA (and thus by inference B219/OB-R promoter activity) in brain was similar in both distribution and relative intensity to OB-R mRNA. OB-RGRP mRNA (and thus by inference OB-R/GRP promoter activity) was widely distributed in murine brain, with elevated expression in the hypothalamic regions that express the leptin receptor mRNA, including the paraventricular nucleus. B219/OB-R 5'-UTR mRNA, but not OB-RGRP mRNA, was upregulated in hypothalamus of obese ob/ob mice. In placenta, B219/OB-R 5'-UTR mRNA was restricted to the maternal interface, and transcription of both long and short leptin receptor splice variants in the main body of the tissue thus proceeds via the OB-R/GRP promoter, strongly indicative of tissue-specific promoter usage.
Original languageEnglish
Pages (from-to)649-655
Number of pages7
JournalJournal of Neuroendocrinology
Volume12
Issue number7
DOIs
Publication statusPublished - 1 Jul 2000

Fingerprint

Leptin Receptors
5' Untranslated Regions
Placenta
Gene Expression
Messenger RNA
Brain
Proteins
Paraventricular Hypothalamic Nucleus
Hypothalamus
In Situ Hybridization
Mothers

Keywords

  • 5' Untranslated Regions
  • Animals
  • Brain
  • Carrier Proteins
  • Female
  • Gene Expression
  • Hypothalamus
  • Mice
  • Mice, Inbred Strains
  • Obesity
  • Placenta
  • Promoter Regions, Genetic
  • RNA, Messenger
  • Receptors, Cell Surface
  • Receptors, Leptin
  • Reference Values

Cite this

@article{3e0eb7e2aa93450cb5a5e27a31ac9b08,
title = "B219/OB-R 5'-UTR and leptin receptor gene-related protein gene expression in mouse brain and placenta tissue-specific leptin receptor promoter activity",
abstract = "Leptin receptor (OB-R) splice variants either encode proteins with different 3' cytoplasmic domains or have different 5' untranslated regions (UTR), indicative of dual promoters. The B219/OB-R promoter transcribes only OB-R transcripts, whereas the OB-R/GRP promoter initiates transcription of both OB-R and another protein of unknown function, called the leptin receptor gene-related protein (OB-RGRP). We compared expression of B219/OB-R 5'-UTR and OB-RGRP mRNAs by in situ hybridization. We thus assessed, by inference, the contributions of the two promoters to the leptin receptor transcript pool, in murine brain or in placenta, a tissue with abundant leptin receptor mRNA. Expression of B219/OB-R 5'-UTR mRNA (and thus by inference B219/OB-R promoter activity) in brain was similar in both distribution and relative intensity to OB-R mRNA. OB-RGRP mRNA (and thus by inference OB-R/GRP promoter activity) was widely distributed in murine brain, with elevated expression in the hypothalamic regions that express the leptin receptor mRNA, including the paraventricular nucleus. B219/OB-R 5'-UTR mRNA, but not OB-RGRP mRNA, was upregulated in hypothalamus of obese ob/ob mice. In placenta, B219/OB-R 5'-UTR mRNA was restricted to the maternal interface, and transcription of both long and short leptin receptor splice variants in the main body of the tissue thus proceeds via the OB-R/GRP promoter, strongly indicative of tissue-specific promoter usage.",
keywords = "5' Untranslated Regions, Animals, Brain, Carrier Proteins, Female, Gene Expression, Hypothalamus, Mice, Mice, Inbred Strains, Obesity, Placenta, Promoter Regions, Genetic, RNA, Messenger, Receptors, Cell Surface, Receptors, Leptin, Reference Values",
author = "Julian Mercer and Kim-Marie Moar and Nigel Hoggard and Strosberg, {A D} and P Froguel and B Bailleul",
year = "2000",
month = "7",
day = "1",
doi = "10.1046/j.1365-2826.2000.00501.x",
language = "English",
volume = "12",
pages = "649--655",
journal = "Journal of Neuroendocrinology",
issn = "0953-8194",
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TY - JOUR

T1 - B219/OB-R 5'-UTR and leptin receptor gene-related protein gene expression in mouse brain and placenta tissue-specific leptin receptor promoter activity

AU - Mercer, Julian

AU - Moar, Kim-Marie

AU - Hoggard, Nigel

AU - Strosberg, A D

AU - Froguel, P

AU - Bailleul, B

PY - 2000/7/1

Y1 - 2000/7/1

N2 - Leptin receptor (OB-R) splice variants either encode proteins with different 3' cytoplasmic domains or have different 5' untranslated regions (UTR), indicative of dual promoters. The B219/OB-R promoter transcribes only OB-R transcripts, whereas the OB-R/GRP promoter initiates transcription of both OB-R and another protein of unknown function, called the leptin receptor gene-related protein (OB-RGRP). We compared expression of B219/OB-R 5'-UTR and OB-RGRP mRNAs by in situ hybridization. We thus assessed, by inference, the contributions of the two promoters to the leptin receptor transcript pool, in murine brain or in placenta, a tissue with abundant leptin receptor mRNA. Expression of B219/OB-R 5'-UTR mRNA (and thus by inference B219/OB-R promoter activity) in brain was similar in both distribution and relative intensity to OB-R mRNA. OB-RGRP mRNA (and thus by inference OB-R/GRP promoter activity) was widely distributed in murine brain, with elevated expression in the hypothalamic regions that express the leptin receptor mRNA, including the paraventricular nucleus. B219/OB-R 5'-UTR mRNA, but not OB-RGRP mRNA, was upregulated in hypothalamus of obese ob/ob mice. In placenta, B219/OB-R 5'-UTR mRNA was restricted to the maternal interface, and transcription of both long and short leptin receptor splice variants in the main body of the tissue thus proceeds via the OB-R/GRP promoter, strongly indicative of tissue-specific promoter usage.

AB - Leptin receptor (OB-R) splice variants either encode proteins with different 3' cytoplasmic domains or have different 5' untranslated regions (UTR), indicative of dual promoters. The B219/OB-R promoter transcribes only OB-R transcripts, whereas the OB-R/GRP promoter initiates transcription of both OB-R and another protein of unknown function, called the leptin receptor gene-related protein (OB-RGRP). We compared expression of B219/OB-R 5'-UTR and OB-RGRP mRNAs by in situ hybridization. We thus assessed, by inference, the contributions of the two promoters to the leptin receptor transcript pool, in murine brain or in placenta, a tissue with abundant leptin receptor mRNA. Expression of B219/OB-R 5'-UTR mRNA (and thus by inference B219/OB-R promoter activity) in brain was similar in both distribution and relative intensity to OB-R mRNA. OB-RGRP mRNA (and thus by inference OB-R/GRP promoter activity) was widely distributed in murine brain, with elevated expression in the hypothalamic regions that express the leptin receptor mRNA, including the paraventricular nucleus. B219/OB-R 5'-UTR mRNA, but not OB-RGRP mRNA, was upregulated in hypothalamus of obese ob/ob mice. In placenta, B219/OB-R 5'-UTR mRNA was restricted to the maternal interface, and transcription of both long and short leptin receptor splice variants in the main body of the tissue thus proceeds via the OB-R/GRP promoter, strongly indicative of tissue-specific promoter usage.

KW - 5' Untranslated Regions

KW - Animals

KW - Brain

KW - Carrier Proteins

KW - Female

KW - Gene Expression

KW - Hypothalamus

KW - Mice

KW - Mice, Inbred Strains

KW - Obesity

KW - Placenta

KW - Promoter Regions, Genetic

KW - RNA, Messenger

KW - Receptors, Cell Surface

KW - Receptors, Leptin

KW - Reference Values

U2 - 10.1046/j.1365-2826.2000.00501.x

DO - 10.1046/j.1365-2826.2000.00501.x

M3 - Article

VL - 12

SP - 649

EP - 655

JO - Journal of Neuroendocrinology

JF - Journal of Neuroendocrinology

SN - 0953-8194

IS - 7

ER -