Bacteria associated with ectomycorrhizas of slash pine (Pinus elliottii) in south-eastern Queensland, Australia

Hironari Izumi, John W. G. Cairney, Ken Killham, Edward Moore, Ian J. Alexander, Ian C. Anderson

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Bacterial communities associated with ectomycorrhizal and uncolonized roots of Pinus elliottii (slash pine) collected from a plantation in south-east Queensland, Australia, were investigated, using cultivation-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene PCR products obtained using a cultivation-independent approach revealed that bacterial communities associated with ectomycorrhizal root tips differed significantly from those associated with roots uncolonized by ectomycorrhizal fungi. DGGE analysis of cultivable bacterial communities revealed no significant difference between ectomycorrhizal and uncolonized roots. Neither analytical approach revealed significant differences between the bacterial communities associated with ectomycorrhizal roots colonized by a Suillus sp. or an Atheliaceae taxon. Cloned bacterial 16S rRNA genes revealed sequence types closely related with that of Burkholderia phenazinium, common in both ectomycorrhizal-colonized and -uncolonized roots, while sequence types most similar to the potentially phyopathogenic bacteria Burkholderia andropogonis and Pantoea ananatis were only detected in ectomycorrhizal roots. These results highlight the possibility of global movement of microorganisms, including putative pathogens, as a result of the introduction of exotic pine plantations.

Original languageEnglish
Pages (from-to)196-204
Number of pages9
JournalFEMS Microbiology Letters
Volume282
Issue number2
Early online date18 Mar 2008
DOIs
Publication statusPublished - May 2008

Keywords

  • endobacteria
  • exotic plantation
  • ectomycorrhizas
  • Burkholderia
  • pine
  • tuber-borchii vittad
  • in-situ detection
  • fungi
  • eucalyptus
  • plantations
  • communities
  • diversity
  • nitrogen
  • radiata

Cite this

Bacteria associated with ectomycorrhizas of slash pine (Pinus elliottii) in south-eastern Queensland, Australia. / Izumi, Hironari; Cairney, John W. G.; Killham, Ken; Moore, Edward; Alexander, Ian J.; Anderson, Ian C.

In: FEMS Microbiology Letters, Vol. 282, No. 2, 05.2008, p. 196-204.

Research output: Contribution to journalArticle

Izumi, Hironari ; Cairney, John W. G. ; Killham, Ken ; Moore, Edward ; Alexander, Ian J. ; Anderson, Ian C. / Bacteria associated with ectomycorrhizas of slash pine (Pinus elliottii) in south-eastern Queensland, Australia. In: FEMS Microbiology Letters. 2008 ; Vol. 282, No. 2. pp. 196-204.
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abstract = "Bacterial communities associated with ectomycorrhizal and uncolonized roots of Pinus elliottii (slash pine) collected from a plantation in south-east Queensland, Australia, were investigated, using cultivation-dependent and -independent methods. Denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA gene PCR products obtained using a cultivation-independent approach revealed that bacterial communities associated with ectomycorrhizal root tips differed significantly from those associated with roots uncolonized by ectomycorrhizal fungi. DGGE analysis of cultivable bacterial communities revealed no significant difference between ectomycorrhizal and uncolonized roots. Neither analytical approach revealed significant differences between the bacterial communities associated with ectomycorrhizal roots colonized by a Suillus sp. or an Atheliaceae taxon. Cloned bacterial 16S rRNA genes revealed sequence types closely related with that of Burkholderia phenazinium, common in both ectomycorrhizal-colonized and -uncolonized roots, while sequence types most similar to the potentially phyopathogenic bacteria Burkholderia andropogonis and Pantoea ananatis were only detected in ectomycorrhizal roots. These results highlight the possibility of global movement of microorganisms, including putative pathogens, as a result of the introduction of exotic pine plantations.",
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