Bacterial microbiota of the upper respiratory tract and childhood asthma

Martin Depner, Markus J Ege, Michael J Cox, Sarah Dwyer, Alan W Walker, Lena T Birzele, Jon Genuneit, Elisabeth Horak, Charlotte Braun-Fahrländer, Hanna Danielewicz, Raina M Maier, Miriam F Moffatt, William O Cookson, Dick Heederik, Erika von Mutius, Antje Legatzki

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

BACKGROUND: Patients with asthma and healthy controls differ in bacterial colonization of the respiratory tract. The upper airways have been shown to reflect colonization of the lower airways, the actual site of inflammation in asthma, which is hardly accessible in population studies.

OBJECTIVE: We sought to characterize the bacterial communities at 2 sites of the upper respiratory tract obtained from children from a rural area and to relate these to asthma.

METHODS: The microbiota of 327 throat and 68 nasal samples from school-age farm and nonfarm children were analyzed by 454-pyrosequencing of the bacterial 16S ribosomal RNA gene.

RESULTS: Alterations in nasal microbiota but not of throat microbiota were associated with asthma. Children with asthma had lower α- and β-diversity of the nasal microbiota as compared with healthy control children. Furthermore, asthma presence was positively associated with a specific operational taxonomic unit from the genus Moraxella in children not exposed to farming, whereas in farm children Moraxella colonization was unrelated to asthma. In nonfarm children, Moraxella colonization explained the association between bacterial diversity and asthma to a large extent.

CONCLUSIONS: Asthma was mainly associated with an altered nasal microbiota characterized by lower diversity and Moraxella abundance. Children living on farms might not be susceptible to the disadvantageous effect of Moraxella. Prospective studies may clarify whether Moraxella outgrowth is a cause or a consequence of loss in diversity.

Original languageEnglish
Pages (from-to)826–834
Number of pages9
JournalJournal of Allergy and Clinical Immunology
Volume139
Issue number3
Early online date27 Jul 2016
DOIs
Publication statusPublished - 1 Mar 2017

Fingerprint

Microbiota
Moraxella
Respiratory System
Asthma
Nose
Pharynx
16S Ribosomal RNA
Agriculture
rRNA Genes
Prospective Studies
Inflammation

Keywords

  • asthma
  • childhood
  • upper respiratory tract
  • throat
  • nose
  • microbiota
  • 16S rRNA gene

ASJC Scopus subject areas

  • Immunology and Microbiology(all)
  • Medicine(all)

Cite this

Bacterial microbiota of the upper respiratory tract and childhood asthma. / Depner, Martin; Ege, Markus J; Cox, Michael J; Dwyer, Sarah; Walker, Alan W; Birzele, Lena T; Genuneit, Jon; Horak, Elisabeth; Braun-Fahrländer, Charlotte; Danielewicz, Hanna; Maier, Raina M; Moffatt, Miriam F; Cookson, William O; Heederik, Dick; von Mutius, Erika; Legatzki, Antje.

In: Journal of Allergy and Clinical Immunology, Vol. 139, No. 3, 01.03.2017, p. 826–834.

Research output: Contribution to journalArticle

Depner, M, Ege, MJ, Cox, MJ, Dwyer, S, Walker, AW, Birzele, LT, Genuneit, J, Horak, E, Braun-Fahrländer, C, Danielewicz, H, Maier, RM, Moffatt, MF, Cookson, WO, Heederik, D, von Mutius, E & Legatzki, A 2017, 'Bacterial microbiota of the upper respiratory tract and childhood asthma', Journal of Allergy and Clinical Immunology, vol. 139, no. 3, pp. 826–834. https://doi.org/10.1016/j.jaci.2016.05.050
Depner, Martin ; Ege, Markus J ; Cox, Michael J ; Dwyer, Sarah ; Walker, Alan W ; Birzele, Lena T ; Genuneit, Jon ; Horak, Elisabeth ; Braun-Fahrländer, Charlotte ; Danielewicz, Hanna ; Maier, Raina M ; Moffatt, Miriam F ; Cookson, William O ; Heederik, Dick ; von Mutius, Erika ; Legatzki, Antje. / Bacterial microbiota of the upper respiratory tract and childhood asthma. In: Journal of Allergy and Clinical Immunology. 2017 ; Vol. 139, No. 3. pp. 826–834.
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T1 - Bacterial microbiota of the upper respiratory tract and childhood asthma

AU - Depner, Martin

AU - Ege, Markus J

AU - Cox, Michael J

AU - Dwyer, Sarah

AU - Walker, Alan W

AU - Birzele, Lena T

AU - Genuneit, Jon

AU - Horak, Elisabeth

AU - Braun-Fahrländer, Charlotte

AU - Danielewicz, Hanna

AU - Maier, Raina M

AU - Moffatt, Miriam F

AU - Cookson, William O

AU - Heederik, Dick

AU - von Mutius, Erika

AU - Legatzki, Antje

N1 - We thank the children who participated in the study, their families, and the field workers participating in GABRIELA. We thank Michele Hoffman, PhD, for help in processing the nasal samples. Throat amplicons were sequenced at the Wellcome Trust Sanger Institute (Cambridge, United Kingdom) and nasal amplicons at the University of Arizona Genomics Institute (Tucson, Arizona).

PY - 2017/3/1

Y1 - 2017/3/1

N2 - BACKGROUND: Patients with asthma and healthy controls differ in bacterial colonization of the respiratory tract. The upper airways have been shown to reflect colonization of the lower airways, the actual site of inflammation in asthma, which is hardly accessible in population studies.OBJECTIVE: We sought to characterize the bacterial communities at 2 sites of the upper respiratory tract obtained from children from a rural area and to relate these to asthma.METHODS: The microbiota of 327 throat and 68 nasal samples from school-age farm and nonfarm children were analyzed by 454-pyrosequencing of the bacterial 16S ribosomal RNA gene.RESULTS: Alterations in nasal microbiota but not of throat microbiota were associated with asthma. Children with asthma had lower α- and β-diversity of the nasal microbiota as compared with healthy control children. Furthermore, asthma presence was positively associated with a specific operational taxonomic unit from the genus Moraxella in children not exposed to farming, whereas in farm children Moraxella colonization was unrelated to asthma. In nonfarm children, Moraxella colonization explained the association between bacterial diversity and asthma to a large extent.CONCLUSIONS: Asthma was mainly associated with an altered nasal microbiota characterized by lower diversity and Moraxella abundance. Children living on farms might not be susceptible to the disadvantageous effect of Moraxella. Prospective studies may clarify whether Moraxella outgrowth is a cause or a consequence of loss in diversity.

AB - BACKGROUND: Patients with asthma and healthy controls differ in bacterial colonization of the respiratory tract. The upper airways have been shown to reflect colonization of the lower airways, the actual site of inflammation in asthma, which is hardly accessible in population studies.OBJECTIVE: We sought to characterize the bacterial communities at 2 sites of the upper respiratory tract obtained from children from a rural area and to relate these to asthma.METHODS: The microbiota of 327 throat and 68 nasal samples from school-age farm and nonfarm children were analyzed by 454-pyrosequencing of the bacterial 16S ribosomal RNA gene.RESULTS: Alterations in nasal microbiota but not of throat microbiota were associated with asthma. Children with asthma had lower α- and β-diversity of the nasal microbiota as compared with healthy control children. Furthermore, asthma presence was positively associated with a specific operational taxonomic unit from the genus Moraxella in children not exposed to farming, whereas in farm children Moraxella colonization was unrelated to asthma. In nonfarm children, Moraxella colonization explained the association between bacterial diversity and asthma to a large extent.CONCLUSIONS: Asthma was mainly associated with an altered nasal microbiota characterized by lower diversity and Moraxella abundance. Children living on farms might not be susceptible to the disadvantageous effect of Moraxella. Prospective studies may clarify whether Moraxella outgrowth is a cause or a consequence of loss in diversity.

KW - asthma

KW - childhood

KW - upper respiratory tract

KW - throat

KW - nose

KW - microbiota

KW - 16S rRNA gene

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SP - 826

EP - 834

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

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ER -