Abstract
A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia toll. Anti-atrazine binding could be observed via an atrazine-alkaline phosphatase conjugate. Cells containing the PAL fusion grew with little cellular toxicity when compared with the control. In contrast, expression of anti-atrazine antibody fragments alone caused the cells to lyse after 4 h. The surface display of anti-pollutant antibodies may have a future role in the bioremediation of contaminated water or the development of pollutant-specific, whole-cell biosensors.
| Original language | English |
|---|---|
| Pages (from-to) | 350-354 |
| Number of pages | 5 |
| Journal | Letters in Applied Microbiology |
| Volume | 28 |
| Publication status | Published - 1999 |
Keywords
- ESCHERICHIA-COLI
- EXPRESSION
- ATRAZINE
- SEPARATION
- PARAQUAT
- CLONING
- PLANTS