Bacterial surface display of an anti-pollutant antibody fragment

J K Dhillon, P D Drew, A J R Porter

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia toll. Anti-atrazine binding could be observed via an atrazine-alkaline phosphatase conjugate. Cells containing the PAL fusion grew with little cellular toxicity when compared with the control. In contrast, expression of anti-atrazine antibody fragments alone caused the cells to lyse after 4 h. The surface display of anti-pollutant antibodies may have a future role in the bioremediation of contaminated water or the development of pollutant-specific, whole-cell biosensors.

Original languageEnglish
Pages (from-to)350-354
Number of pages5
JournalLetters in Applied Microbiology
Volume28
Publication statusPublished - 1999

Keywords

  • ESCHERICHIA-COLI
  • EXPRESSION
  • ATRAZINE
  • SEPARATION
  • PARAQUAT
  • CLONING
  • PLANTS

Cite this

Bacterial surface display of an anti-pollutant antibody fragment. / Dhillon, J K ; Drew, P D ; Porter, A J R .

In: Letters in Applied Microbiology, Vol. 28, 1999, p. 350-354.

Research output: Contribution to journalArticle

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AU - Drew, P D

AU - Porter, A J R

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AB - A peptidoglycan-associated lipoprotein (PAL) fused to an antibody fragment (scFv) specific to the herbicide and environmental pollutant atrazine, has been successfully targeted to the cell surface of Escherichia toll. Anti-atrazine binding could be observed via an atrazine-alkaline phosphatase conjugate. Cells containing the PAL fusion grew with little cellular toxicity when compared with the control. In contrast, expression of anti-atrazine antibody fragments alone caused the cells to lyse after 4 h. The surface display of anti-pollutant antibodies may have a future role in the bioremediation of contaminated water or the development of pollutant-specific, whole-cell biosensors.

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KW - SEPARATION

KW - PARAQUAT

KW - CLONING

KW - PLANTS

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JO - Letters in Applied Microbiology

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