Abstract
Rhizodeposition is an important source of substrate for microbial communities, supporting activities including soil organic matter (SOM) and nutrient cycling. Therefore, it is a potential trait of interest for crop plants, particularly in the context of variety selection for sustainable production systems. However, we do not have a good understanding of (i) whether there is significant variation in root-C deposition between varieties of important agricultural crops and (ii) whether variation in C deposition between varieties leads to major differences in C cycling in soil. In two experiments, we assessed variations in C deposition amongst barley genotypes and their respective impacts on microbial activity and SOM dynamics. In experiment 1, we applied C-13-CO2 labelling to selected barley recombinant chromosome substitution lines (RCSLs) and traced root-derived C in surface soil CO2 efflux, soil microbial biomass-C (MBC), soil solution, and soil particle-size fractions. In experiment 2, we conducted MicroResp analysis using 15 ecologically relevant C substrates to assess the impacts of barley genotypes on microbial activity. Soil respiration measurements (partitioned into plant- and SOM-derived components) revealed genotype-specific effects on plant-derived C, SOM-derived C and total C respired as CO2. For particle-size fractionation, we found that incorporation of plant-derived C to the silt-and-clay fraction varied between genotypes, indicating differences in relative stabilization of root-derived C as a result of barley genotype. Our data did not indicate genotype effects on total MBC size or dissolved organic-C (DOC) in soil solutions, but significant differences in plant-derived MBC and DOC were observed. MicroResp analysis showed differential utilization of 7 substrates (glucose, trehalose, lignin, arabinose, alanine, aminobutyric acid and lysine) revealing variation in community level physiological profiles (CLPPs) of soil microbes as impacted by barley genotypes. Furthermore, we found significant clustering of microbial CLPPs as a function of RCSLs and parent lines (Caesarea 26-24 and Harrington) suggesting a strong plant genetic control of the barley microbiome, and that this genetic control is heritable. Our results demonstrate barley genotype-specific effects on soil processes, revealing the potential for germplasm selection and variety improvement in barley to support sustainable production systems. (C) 2015 Elsevier Ltd. All rights reserved.
| Original language | English |
|---|---|
| Pages (from-to) | 60-69 |
| Number of pages | 10 |
| Journal | Soil Biology and Biochemistry |
| Volume | 95 |
| Early online date | 5 Jan 2016 |
| DOIs | |
| Publication status | Published - Apr 2016 |
Keywords
- C stabilization
- microbial community level physiological profiles
- rhizosphere
- soil organic matter priming
- variety selection
- C-13 tracing
- chromosome substitution Lines
- greenhouse-gas mitigation
- microbial communities
- land-use
- carbon sequestration
- mycorrhizal fungi
- extraction method
- plant
- roots
Cite this
Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization. / Mwafulirwa, Lumbani; Baggs, Elizabeth M.; Russell, Joanne; George, Timothy; Morley, Nicholas; Sim, Allan; Canto, Carla de la Fuente; Paterson, Eric.
In: Soil Biology and Biochemistry, Vol. 95, 04.2016, p. 60-69.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization
AU - Mwafulirwa, Lumbani
AU - Baggs, Elizabeth M.
AU - Russell, Joanne
AU - George, Timothy
AU - Morley, Nicholas
AU - Sim, Allan
AU - Canto, Carla de la Fuente
AU - Paterson, Eric
N1 - Acknowledgements We thank B. Thornton, G. Martin, M. Procee, Y. Cook, and S. McIntyre who conducted specialist analytical procedures and J. Garcia-Pausas, C. Murphy, J. Fisher, L. Brown, and C. Ghee for their respective inputs towards laboratory activities. We also acknowledge P. Hayes and I. Matus for providing the original RCSL collection, B. Duff whom we consulted for statistical matters and two anonymous reviewers who provided constructive comments to improve the paper. This work was funded by a James Hutton Institute international PhD studentship awarded to L. Mwafulirwa.
PY - 2016/4
Y1 - 2016/4
N2 - Rhizodeposition is an important source of substrate for microbial communities, supporting activities including soil organic matter (SOM) and nutrient cycling. Therefore, it is a potential trait of interest for crop plants, particularly in the context of variety selection for sustainable production systems. However, we do not have a good understanding of (i) whether there is significant variation in root-C deposition between varieties of important agricultural crops and (ii) whether variation in C deposition between varieties leads to major differences in C cycling in soil. In two experiments, we assessed variations in C deposition amongst barley genotypes and their respective impacts on microbial activity and SOM dynamics. In experiment 1, we applied C-13-CO2 labelling to selected barley recombinant chromosome substitution lines (RCSLs) and traced root-derived C in surface soil CO2 efflux, soil microbial biomass-C (MBC), soil solution, and soil particle-size fractions. In experiment 2, we conducted MicroResp analysis using 15 ecologically relevant C substrates to assess the impacts of barley genotypes on microbial activity. Soil respiration measurements (partitioned into plant- and SOM-derived components) revealed genotype-specific effects on plant-derived C, SOM-derived C and total C respired as CO2. For particle-size fractionation, we found that incorporation of plant-derived C to the silt-and-clay fraction varied between genotypes, indicating differences in relative stabilization of root-derived C as a result of barley genotype. Our data did not indicate genotype effects on total MBC size or dissolved organic-C (DOC) in soil solutions, but significant differences in plant-derived MBC and DOC were observed. MicroResp analysis showed differential utilization of 7 substrates (glucose, trehalose, lignin, arabinose, alanine, aminobutyric acid and lysine) revealing variation in community level physiological profiles (CLPPs) of soil microbes as impacted by barley genotypes. Furthermore, we found significant clustering of microbial CLPPs as a function of RCSLs and parent lines (Caesarea 26-24 and Harrington) suggesting a strong plant genetic control of the barley microbiome, and that this genetic control is heritable. Our results demonstrate barley genotype-specific effects on soil processes, revealing the potential for germplasm selection and variety improvement in barley to support sustainable production systems. (C) 2015 Elsevier Ltd. All rights reserved.
AB - Rhizodeposition is an important source of substrate for microbial communities, supporting activities including soil organic matter (SOM) and nutrient cycling. Therefore, it is a potential trait of interest for crop plants, particularly in the context of variety selection for sustainable production systems. However, we do not have a good understanding of (i) whether there is significant variation in root-C deposition between varieties of important agricultural crops and (ii) whether variation in C deposition between varieties leads to major differences in C cycling in soil. In two experiments, we assessed variations in C deposition amongst barley genotypes and their respective impacts on microbial activity and SOM dynamics. In experiment 1, we applied C-13-CO2 labelling to selected barley recombinant chromosome substitution lines (RCSLs) and traced root-derived C in surface soil CO2 efflux, soil microbial biomass-C (MBC), soil solution, and soil particle-size fractions. In experiment 2, we conducted MicroResp analysis using 15 ecologically relevant C substrates to assess the impacts of barley genotypes on microbial activity. Soil respiration measurements (partitioned into plant- and SOM-derived components) revealed genotype-specific effects on plant-derived C, SOM-derived C and total C respired as CO2. For particle-size fractionation, we found that incorporation of plant-derived C to the silt-and-clay fraction varied between genotypes, indicating differences in relative stabilization of root-derived C as a result of barley genotype. Our data did not indicate genotype effects on total MBC size or dissolved organic-C (DOC) in soil solutions, but significant differences in plant-derived MBC and DOC were observed. MicroResp analysis showed differential utilization of 7 substrates (glucose, trehalose, lignin, arabinose, alanine, aminobutyric acid and lysine) revealing variation in community level physiological profiles (CLPPs) of soil microbes as impacted by barley genotypes. Furthermore, we found significant clustering of microbial CLPPs as a function of RCSLs and parent lines (Caesarea 26-24 and Harrington) suggesting a strong plant genetic control of the barley microbiome, and that this genetic control is heritable. Our results demonstrate barley genotype-specific effects on soil processes, revealing the potential for germplasm selection and variety improvement in barley to support sustainable production systems. (C) 2015 Elsevier Ltd. All rights reserved.
KW - C stabilization
KW - microbial community level physiological profiles
KW - rhizosphere
KW - soil organic matter priming
KW - variety selection
KW - C-13 tracing
KW - chromosome substitution Lines
KW - greenhouse-gas mitigation
KW - microbial communities
KW - land-use
KW - carbon sequestration
KW - mycorrhizal fungi
KW - extraction method
KW - plant
KW - roots
U2 - 10.1016/j.soilbio.2015.12.011
DO - 10.1016/j.soilbio.2015.12.011
M3 - Article
VL - 95
SP - 60
EP - 69
JO - Soil Biology and Biochemistry
JF - Soil Biology and Biochemistry
SN - 0038-0717
ER -