Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization

Lumbani Mwafulirwa*, Elizabeth M. Baggs, Joanne Russell, Timothy George, Nicholas Morley, Allan Sim, Carla de la Fuente Canto, Eric Paterson

*Corresponding author for this work

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Rhizodeposition is an important source of substrate for microbial communities, supporting activities including soil organic matter (SOM) and nutrient cycling. Therefore, it is a potential trait of interest for crop plants, particularly in the context of variety selection for sustainable production systems. However, we do not have a good understanding of (i) whether there is significant variation in root-C deposition between varieties of important agricultural crops and (ii) whether variation in C deposition between varieties leads to major differences in C cycling in soil. In two experiments, we assessed variations in C deposition amongst barley genotypes and their respective impacts on microbial activity and SOM dynamics. In experiment 1, we applied C-13-CO2 labelling to selected barley recombinant chromosome substitution lines (RCSLs) and traced root-derived C in surface soil CO2 efflux, soil microbial biomass-C (MBC), soil solution, and soil particle-size fractions. In experiment 2, we conducted MicroResp analysis using 15 ecologically relevant C substrates to assess the impacts of barley genotypes on microbial activity. Soil respiration measurements (partitioned into plant- and SOM-derived components) revealed genotype-specific effects on plant-derived C, SOM-derived C and total C respired as CO2. For particle-size fractionation, we found that incorporation of plant-derived C to the silt-and-clay fraction varied between genotypes, indicating differences in relative stabilization of root-derived C as a result of barley genotype. Our data did not indicate genotype effects on total MBC size or dissolved organic-C (DOC) in soil solutions, but significant differences in plant-derived MBC and DOC were observed. MicroResp analysis showed differential utilization of 7 substrates (glucose, trehalose, lignin, arabinose, alanine, aminobutyric acid and lysine) revealing variation in community level physiological profiles (CLPPs) of soil microbes as impacted by barley genotypes. Furthermore, we found significant clustering of microbial CLPPs as a function of RCSLs and parent lines (Caesarea 26-24 and Harrington) suggesting a strong plant genetic control of the barley microbiome, and that this genetic control is heritable. Our results demonstrate barley genotype-specific effects on soil processes, revealing the potential for germplasm selection and variety improvement in barley to support sustainable production systems. (C) 2015 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)60-69
Number of pages10
JournalSoil Biology and Biochemistry
Volume95
Early online date5 Jan 2016
DOIs
Publication statusPublished - Apr 2016

Keywords

  • C stabilization
  • microbial community level physiological profiles
  • rhizosphere
  • soil organic matter priming
  • variety selection
  • C-13 tracing
  • chromosome substitution Lines
  • greenhouse-gas mitigation
  • microbial communities
  • land-use
  • carbon sequestration
  • mycorrhizal fungi
  • extraction method
  • plant
  • roots

Cite this

Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization. / Mwafulirwa, Lumbani; Baggs, Elizabeth M.; Russell, Joanne; George, Timothy; Morley, Nicholas; Sim, Allan; Canto, Carla de la Fuente; Paterson, Eric.

In: Soil Biology and Biochemistry, Vol. 95, 04.2016, p. 60-69.

Research output: Contribution to journalArticle

Mwafulirwa, L, Baggs, EM, Russell, J, George, T, Morley, N, Sim, A, Canto, CDLF & Paterson, E 2016, 'Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization', Soil Biology and Biochemistry, vol. 95, pp. 60-69. https://doi.org/10.1016/j.soilbio.2015.12.011
Mwafulirwa, Lumbani ; Baggs, Elizabeth M. ; Russell, Joanne ; George, Timothy ; Morley, Nicholas ; Sim, Allan ; Canto, Carla de la Fuente ; Paterson, Eric. / Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization. In: Soil Biology and Biochemistry. 2016 ; Vol. 95. pp. 60-69.
@article{c061427f1b374981bbd5487234cbe5c2,
title = "Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization",
abstract = "Rhizodeposition is an important source of substrate for microbial communities, supporting activities including soil organic matter (SOM) and nutrient cycling. Therefore, it is a potential trait of interest for crop plants, particularly in the context of variety selection for sustainable production systems. However, we do not have a good understanding of (i) whether there is significant variation in root-C deposition between varieties of important agricultural crops and (ii) whether variation in C deposition between varieties leads to major differences in C cycling in soil. In two experiments, we assessed variations in C deposition amongst barley genotypes and their respective impacts on microbial activity and SOM dynamics. In experiment 1, we applied C-13-CO2 labelling to selected barley recombinant chromosome substitution lines (RCSLs) and traced root-derived C in surface soil CO2 efflux, soil microbial biomass-C (MBC), soil solution, and soil particle-size fractions. In experiment 2, we conducted MicroResp analysis using 15 ecologically relevant C substrates to assess the impacts of barley genotypes on microbial activity. Soil respiration measurements (partitioned into plant- and SOM-derived components) revealed genotype-specific effects on plant-derived C, SOM-derived C and total C respired as CO2. For particle-size fractionation, we found that incorporation of plant-derived C to the silt-and-clay fraction varied between genotypes, indicating differences in relative stabilization of root-derived C as a result of barley genotype. Our data did not indicate genotype effects on total MBC size or dissolved organic-C (DOC) in soil solutions, but significant differences in plant-derived MBC and DOC were observed. MicroResp analysis showed differential utilization of 7 substrates (glucose, trehalose, lignin, arabinose, alanine, aminobutyric acid and lysine) revealing variation in community level physiological profiles (CLPPs) of soil microbes as impacted by barley genotypes. Furthermore, we found significant clustering of microbial CLPPs as a function of RCSLs and parent lines (Caesarea 26-24 and Harrington) suggesting a strong plant genetic control of the barley microbiome, and that this genetic control is heritable. Our results demonstrate barley genotype-specific effects on soil processes, revealing the potential for germplasm selection and variety improvement in barley to support sustainable production systems. (C) 2015 Elsevier Ltd. All rights reserved.",
keywords = "C stabilization, microbial community level physiological profiles, rhizosphere, soil organic matter priming, variety selection, C-13 tracing, chromosome substitution Lines, greenhouse-gas mitigation, microbial communities, land-use, carbon sequestration, mycorrhizal fungi, extraction method, plant, roots",
author = "Lumbani Mwafulirwa and Baggs, {Elizabeth M.} and Joanne Russell and Timothy George and Nicholas Morley and Allan Sim and Canto, {Carla de la Fuente} and Eric Paterson",
note = "Acknowledgements We thank B. Thornton, G. Martin, M. Procee, Y. Cook, and S. McIntyre who conducted specialist analytical procedures and J. Garcia-Pausas, C. Murphy, J. Fisher, L. Brown, and C. Ghee for their respective inputs towards laboratory activities. We also acknowledge P. Hayes and I. Matus for providing the original RCSL collection, B. Duff whom we consulted for statistical matters and two anonymous reviewers who provided constructive comments to improve the paper. This work was funded by a James Hutton Institute international PhD studentship awarded to L. Mwafulirwa.",
year = "2016",
month = "4",
doi = "10.1016/j.soilbio.2015.12.011",
language = "English",
volume = "95",
pages = "60--69",
journal = "Soil Biology and Biochemistry",
issn = "0038-0717",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Barley genotype influences stabilization of rhizodeposition-derived C and soil organic matter mineralization

AU - Mwafulirwa, Lumbani

AU - Baggs, Elizabeth M.

AU - Russell, Joanne

AU - George, Timothy

AU - Morley, Nicholas

AU - Sim, Allan

AU - Canto, Carla de la Fuente

AU - Paterson, Eric

N1 - Acknowledgements We thank B. Thornton, G. Martin, M. Procee, Y. Cook, and S. McIntyre who conducted specialist analytical procedures and J. Garcia-Pausas, C. Murphy, J. Fisher, L. Brown, and C. Ghee for their respective inputs towards laboratory activities. We also acknowledge P. Hayes and I. Matus for providing the original RCSL collection, B. Duff whom we consulted for statistical matters and two anonymous reviewers who provided constructive comments to improve the paper. This work was funded by a James Hutton Institute international PhD studentship awarded to L. Mwafulirwa.

PY - 2016/4

Y1 - 2016/4

N2 - Rhizodeposition is an important source of substrate for microbial communities, supporting activities including soil organic matter (SOM) and nutrient cycling. Therefore, it is a potential trait of interest for crop plants, particularly in the context of variety selection for sustainable production systems. However, we do not have a good understanding of (i) whether there is significant variation in root-C deposition between varieties of important agricultural crops and (ii) whether variation in C deposition between varieties leads to major differences in C cycling in soil. In two experiments, we assessed variations in C deposition amongst barley genotypes and their respective impacts on microbial activity and SOM dynamics. In experiment 1, we applied C-13-CO2 labelling to selected barley recombinant chromosome substitution lines (RCSLs) and traced root-derived C in surface soil CO2 efflux, soil microbial biomass-C (MBC), soil solution, and soil particle-size fractions. In experiment 2, we conducted MicroResp analysis using 15 ecologically relevant C substrates to assess the impacts of barley genotypes on microbial activity. Soil respiration measurements (partitioned into plant- and SOM-derived components) revealed genotype-specific effects on plant-derived C, SOM-derived C and total C respired as CO2. For particle-size fractionation, we found that incorporation of plant-derived C to the silt-and-clay fraction varied between genotypes, indicating differences in relative stabilization of root-derived C as a result of barley genotype. Our data did not indicate genotype effects on total MBC size or dissolved organic-C (DOC) in soil solutions, but significant differences in plant-derived MBC and DOC were observed. MicroResp analysis showed differential utilization of 7 substrates (glucose, trehalose, lignin, arabinose, alanine, aminobutyric acid and lysine) revealing variation in community level physiological profiles (CLPPs) of soil microbes as impacted by barley genotypes. Furthermore, we found significant clustering of microbial CLPPs as a function of RCSLs and parent lines (Caesarea 26-24 and Harrington) suggesting a strong plant genetic control of the barley microbiome, and that this genetic control is heritable. Our results demonstrate barley genotype-specific effects on soil processes, revealing the potential for germplasm selection and variety improvement in barley to support sustainable production systems. (C) 2015 Elsevier Ltd. All rights reserved.

AB - Rhizodeposition is an important source of substrate for microbial communities, supporting activities including soil organic matter (SOM) and nutrient cycling. Therefore, it is a potential trait of interest for crop plants, particularly in the context of variety selection for sustainable production systems. However, we do not have a good understanding of (i) whether there is significant variation in root-C deposition between varieties of important agricultural crops and (ii) whether variation in C deposition between varieties leads to major differences in C cycling in soil. In two experiments, we assessed variations in C deposition amongst barley genotypes and their respective impacts on microbial activity and SOM dynamics. In experiment 1, we applied C-13-CO2 labelling to selected barley recombinant chromosome substitution lines (RCSLs) and traced root-derived C in surface soil CO2 efflux, soil microbial biomass-C (MBC), soil solution, and soil particle-size fractions. In experiment 2, we conducted MicroResp analysis using 15 ecologically relevant C substrates to assess the impacts of barley genotypes on microbial activity. Soil respiration measurements (partitioned into plant- and SOM-derived components) revealed genotype-specific effects on plant-derived C, SOM-derived C and total C respired as CO2. For particle-size fractionation, we found that incorporation of plant-derived C to the silt-and-clay fraction varied between genotypes, indicating differences in relative stabilization of root-derived C as a result of barley genotype. Our data did not indicate genotype effects on total MBC size or dissolved organic-C (DOC) in soil solutions, but significant differences in plant-derived MBC and DOC were observed. MicroResp analysis showed differential utilization of 7 substrates (glucose, trehalose, lignin, arabinose, alanine, aminobutyric acid and lysine) revealing variation in community level physiological profiles (CLPPs) of soil microbes as impacted by barley genotypes. Furthermore, we found significant clustering of microbial CLPPs as a function of RCSLs and parent lines (Caesarea 26-24 and Harrington) suggesting a strong plant genetic control of the barley microbiome, and that this genetic control is heritable. Our results demonstrate barley genotype-specific effects on soil processes, revealing the potential for germplasm selection and variety improvement in barley to support sustainable production systems. (C) 2015 Elsevier Ltd. All rights reserved.

KW - C stabilization

KW - microbial community level physiological profiles

KW - rhizosphere

KW - soil organic matter priming

KW - variety selection

KW - C-13 tracing

KW - chromosome substitution Lines

KW - greenhouse-gas mitigation

KW - microbial communities

KW - land-use

KW - carbon sequestration

KW - mycorrhizal fungi

KW - extraction method

KW - plant

KW - roots

U2 - 10.1016/j.soilbio.2015.12.011

DO - 10.1016/j.soilbio.2015.12.011

M3 - Article

VL - 95

SP - 60

EP - 69

JO - Soil Biology and Biochemistry

JF - Soil Biology and Biochemistry

SN - 0038-0717

ER -