Binding of soluble immune complexes to fractionated Atlantic salmon (Salmo salar L.) leucocytes

A M O'Dowd, A E Ellis, C J Secombes

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6 Citations (Scopus)

Abstract

Binding of a fluorescent-labelled soluble immune complex to different types of Atlantic salmon leucocytes was investigated using flow cytometry. Peripheral blood leucocytes (PBL) were separated into sIg(+) and sIg(-) enriched populations by magnetic activated cell sorting, blood neutrophils were identified by electronic gating, and kidney macrophages were selected by plastic-adherence. About 60% of both sIg(+) and sIg(-) enriched PBL, 44% of neutrophils and 34% of macrophages bound the soluble immune complexes. (C) 1999 Elsevier Science B.V. All rights reserved.

Original languageEnglish
Pages (from-to)149-157
Number of pages9
JournalVeterinary Immunology and Immunopathology
Volume68
Publication statusPublished - 1999

Keywords

  • Atlantic salmon
  • B lymphocytes
  • flow cytometry
  • immune complexes
  • macrophages
  • neutrophils
  • FC-GAMMA RECEPTORS
  • RAINBOW-TROUT
  • ONCORHYNCHUS-MYKISS
  • PROLIFERATIVE RESPONSES
  • AEROMONAS-SALMONICIDA
  • MONOCLONAL-ANTIBODIES
  • LEUKOCYTES
  • IGM
  • PHAGOCYTOSIS
  • EXPRESSION

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