Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material

L J Shaw, Y Beaton, L A Glover, K Killham, D Osborn, A A Meharg

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Interaction of organic xenobiotics with soil water-soluble humic material (WSHM) may influence their environmental fate and bioavailability. We utilized bacterial assays (lux-based toxicity and mineralization by Burkholderia sp. RASC) to assess temporal changes in the bioavailability of [C-14]-2,4-dichlorophenol (2,4-DCP) in soil water extracts (29.5 pg mL(-1) 2,4-DCP; 840.2 mug mL(-1) organic carbon). HPLC determined and bioavailable concentrations were compared. Gel permeation chromatography (GPC) was used to confirm the association of a fraction (>50%) of [C-14]-2,4-DCP with WSHM. Subtle differences in pa ra meters describing 2,4-DCP mineralization curves were recorded for different soil-2,4-DCP contact times. Problems regarding the interpretation of mineralization data when assessing the bioavailability of toxic compounds are discussed. The lux-bioassay revealed a time-dependent reduction in 2,4-DCP bioavailability: after 7 d, less than 20% was bioavailable, However, GPC showed no quantitative difference in the amount of WSHM-associated 2,4-DCP over this time. These data suggest qualitative changes in the nature of the 2,4-DCP-WSHM association and that associated 2,4-DCP may exert a toxic effect. Although GPC distinguished between free- and WSHM-associated 2,4-DCP, it did not resolve the temporal shift in bioavailability revealed by the lux biosensor. These results stress that assessment of risk posed by chemicals must be considered using appropriate biological assays.

Original languageEnglish
Pages (from-to)4721-4726
Number of pages6
JournalEnvironmental Science & Technology
Volume34
Publication statusPublished - 2000

Keywords

  • GEL-PERMEATION CHROMATOGRAPHY
  • ENERGY-TRANSDUCING MEMBRANES
  • ORGANIC-MATTER
  • MACROMOLECULAR STRUCTURES
  • PSEUDOMONAS-FLUORESCENS
  • SORPTION
  • ACID
  • BIODEGRADATION
  • CHEMICALS
  • TOXICITY

Cite this

Shaw, L. J., Beaton, Y., Glover, L. A., Killham, K., Osborn, D., & Meharg, A. A. (2000). Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material. Environmental Science & Technology, 34, 4721-4726.

Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material. / Shaw, L J ; Beaton, Y ; Glover, L A ; Killham, K ; Osborn, D ; Meharg, A A .

In: Environmental Science & Technology, Vol. 34, 2000, p. 4721-4726.

Research output: Contribution to journalArticle

Shaw, LJ, Beaton, Y, Glover, LA, Killham, K, Osborn, D & Meharg, AA 2000, 'Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material', Environmental Science & Technology, vol. 34, pp. 4721-4726.
Shaw LJ, Beaton Y, Glover LA, Killham K, Osborn D, Meharg AA. Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material. Environmental Science & Technology. 2000;34:4721-4726.
Shaw, L J ; Beaton, Y ; Glover, L A ; Killham, K ; Osborn, D ; Meharg, A A . / Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material. In: Environmental Science & Technology. 2000 ; Vol. 34. pp. 4721-4726.
@article{b7667eb83802492586aaa92b044348de,
title = "Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material",
abstract = "Interaction of organic xenobiotics with soil water-soluble humic material (WSHM) may influence their environmental fate and bioavailability. We utilized bacterial assays (lux-based toxicity and mineralization by Burkholderia sp. RASC) to assess temporal changes in the bioavailability of [C-14]-2,4-dichlorophenol (2,4-DCP) in soil water extracts (29.5 pg mL(-1) 2,4-DCP; 840.2 mug mL(-1) organic carbon). HPLC determined and bioavailable concentrations were compared. Gel permeation chromatography (GPC) was used to confirm the association of a fraction (>50{\%}) of [C-14]-2,4-DCP with WSHM. Subtle differences in pa ra meters describing 2,4-DCP mineralization curves were recorded for different soil-2,4-DCP contact times. Problems regarding the interpretation of mineralization data when assessing the bioavailability of toxic compounds are discussed. The lux-bioassay revealed a time-dependent reduction in 2,4-DCP bioavailability: after 7 d, less than 20{\%} was bioavailable, However, GPC showed no quantitative difference in the amount of WSHM-associated 2,4-DCP over this time. These data suggest qualitative changes in the nature of the 2,4-DCP-WSHM association and that associated 2,4-DCP may exert a toxic effect. Although GPC distinguished between free- and WSHM-associated 2,4-DCP, it did not resolve the temporal shift in bioavailability revealed by the lux biosensor. These results stress that assessment of risk posed by chemicals must be considered using appropriate biological assays.",
keywords = "GEL-PERMEATION CHROMATOGRAPHY, ENERGY-TRANSDUCING MEMBRANES, ORGANIC-MATTER, MACROMOLECULAR STRUCTURES, PSEUDOMONAS-FLUORESCENS, SORPTION, ACID, BIODEGRADATION, CHEMICALS, TOXICITY",
author = "Shaw, {L J} and Y Beaton and Glover, {L A} and K Killham and D Osborn and Meharg, {A A}",
year = "2000",
language = "English",
volume = "34",
pages = "4721--4726",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",

}

TY - JOUR

T1 - Bioavailability of 2,4-dichlorophenol associated with soil water-soluble humic material

AU - Shaw, L J

AU - Beaton, Y

AU - Glover, L A

AU - Killham, K

AU - Osborn, D

AU - Meharg, A A

PY - 2000

Y1 - 2000

N2 - Interaction of organic xenobiotics with soil water-soluble humic material (WSHM) may influence their environmental fate and bioavailability. We utilized bacterial assays (lux-based toxicity and mineralization by Burkholderia sp. RASC) to assess temporal changes in the bioavailability of [C-14]-2,4-dichlorophenol (2,4-DCP) in soil water extracts (29.5 pg mL(-1) 2,4-DCP; 840.2 mug mL(-1) organic carbon). HPLC determined and bioavailable concentrations were compared. Gel permeation chromatography (GPC) was used to confirm the association of a fraction (>50%) of [C-14]-2,4-DCP with WSHM. Subtle differences in pa ra meters describing 2,4-DCP mineralization curves were recorded for different soil-2,4-DCP contact times. Problems regarding the interpretation of mineralization data when assessing the bioavailability of toxic compounds are discussed. The lux-bioassay revealed a time-dependent reduction in 2,4-DCP bioavailability: after 7 d, less than 20% was bioavailable, However, GPC showed no quantitative difference in the amount of WSHM-associated 2,4-DCP over this time. These data suggest qualitative changes in the nature of the 2,4-DCP-WSHM association and that associated 2,4-DCP may exert a toxic effect. Although GPC distinguished between free- and WSHM-associated 2,4-DCP, it did not resolve the temporal shift in bioavailability revealed by the lux biosensor. These results stress that assessment of risk posed by chemicals must be considered using appropriate biological assays.

AB - Interaction of organic xenobiotics with soil water-soluble humic material (WSHM) may influence their environmental fate and bioavailability. We utilized bacterial assays (lux-based toxicity and mineralization by Burkholderia sp. RASC) to assess temporal changes in the bioavailability of [C-14]-2,4-dichlorophenol (2,4-DCP) in soil water extracts (29.5 pg mL(-1) 2,4-DCP; 840.2 mug mL(-1) organic carbon). HPLC determined and bioavailable concentrations were compared. Gel permeation chromatography (GPC) was used to confirm the association of a fraction (>50%) of [C-14]-2,4-DCP with WSHM. Subtle differences in pa ra meters describing 2,4-DCP mineralization curves were recorded for different soil-2,4-DCP contact times. Problems regarding the interpretation of mineralization data when assessing the bioavailability of toxic compounds are discussed. The lux-bioassay revealed a time-dependent reduction in 2,4-DCP bioavailability: after 7 d, less than 20% was bioavailable, However, GPC showed no quantitative difference in the amount of WSHM-associated 2,4-DCP over this time. These data suggest qualitative changes in the nature of the 2,4-DCP-WSHM association and that associated 2,4-DCP may exert a toxic effect. Although GPC distinguished between free- and WSHM-associated 2,4-DCP, it did not resolve the temporal shift in bioavailability revealed by the lux biosensor. These results stress that assessment of risk posed by chemicals must be considered using appropriate biological assays.

KW - GEL-PERMEATION CHROMATOGRAPHY

KW - ENERGY-TRANSDUCING MEMBRANES

KW - ORGANIC-MATTER

KW - MACROMOLECULAR STRUCTURES

KW - PSEUDOMONAS-FLUORESCENS

KW - SORPTION

KW - ACID

KW - BIODEGRADATION

KW - CHEMICALS

KW - TOXICITY

M3 - Article

VL - 34

SP - 4721

EP - 4726

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

ER -