Biochemical characterization of recombinant Candida albicans mannosyltransferases Mnt1, Mnt2 and Mnt5 reveals new functions in O- and N-mannan biosynthesis

Diana F. Diaz-Jimenez, Hector M. Mora-Montes, Arturo Hernandez-Cervantes, Juan P. Luna-Arias, Neil A. R. Gow, Arturo Flores-Carreon

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)
6 Downloads (Pure)

Abstract

The cell surface of Candida albicans is enriched with highly glycosylated mannoproteins that are involved in the interaction with host tissues. N- and O-glycosylation are post-translational modifications that initiate in the endoplasmic reticulum, and finalize in the Golgi. The KRE2/MNT1 family encode a set of multifunctional mannosyltransferases that participate in O-, N- and phosphomannosylation. In order to gain insights into the substrate specificities of these enzymes, recombinant forms of Mnt1. Mnt2, and Mnt5 were expressed in Pichia pastoris and the enzyme activities characterized. Mnt1 and Mnt2 showed a high specificity for alpha-methylmannoside and alpha 1,2-mannobiose as acceptor substrates. Notably, they also used Saccharomyces cerevisiae O-mannans as acceptors and generated products with more than three mannose residues, suggesting than Mnt1 and Mnt2 could be the mannosyltransferases adding the fourth and fifth mannose residue to the O-mannans in C albicans. Mnt5 only recognized alpha-mannans as acceptor, suggesting that participates in the addition of the second mannose residues to the N-glycan outer chain. (C) 2012 Elsevier Inc. All rights reserved.

Original languageEnglish
Pages (from-to)77-82
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume419
Issue number1
DOIs
Publication statusPublished - 2 Mar 2012

Keywords

  • candida albicans
  • mannosyltransferases
  • glycosylation
  • cell-wall integrity
  • saccharomyces-cerevisiae
  • pichia-pastoris
  • protein mannosyltransferase
  • antifungal resistance
  • virulence
  • alpha-1,2-mannosyltransferase
  • morphogenesis
  • recognition

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