Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1 beta

F Buonocore, M Forlenza, E Randelli, S Benedetti, P Bossu, S Meloni, C J Secombes, M Mazzini, G Scapigliati

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Abstract

Biological activities of a putative mature sea bass interleukin-1 beta peptide, produced as a recombinant protein (rIL-1 beta) in Escherichia coli, have been investigated. The rIL-1 beta contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1 beta induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1 beta administered intraperitoneally increased expression levels of the IL-1 beta gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.

Original languageEnglish
Pages (from-to)609-617
Number of pages9
JournalMarine Biotechnology
Volume7
DOIs
Publication statusPublished - 2005

Keywords

  • European sea bass
  • cytokine
  • interleukin 1 beta
  • proliferation
  • phagocytosis
  • cyclooxygenase 2
  • TROUT ONCORHYNCHUS-MYKISS
  • RAINBOW-TROUT
  • EXPRESSION ANALYSIS
  • MOLECULAR-CLONING
  • RECEPTOR
  • IL-1-BETA
  • FISH
  • CARP
  • MACROPHAGES
  • ACTIVATION

Cite this

Buonocore, F., Forlenza, M., Randelli, E., Benedetti, S., Bossu, P., Meloni, S., Secombes, C. J., Mazzini, M., & Scapigliati, G. (2005). Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1 beta. Marine Biotechnology, 7, 609-617. https://doi.org/10.1007/s10126-004-5131-5