Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1 beta

F  Buonocore; M  Forlenza; E  Randelli; S  Benedetti; P  Bossu; S  Meloni; C J  Secombes; M  Mazzini; G  Scapigliati

doi:10.1007/s10126-004-5131-5

Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1 beta

F Buonocore, M Forlenza, E Randelli, S Benedetti, P Bossu, S Meloni, C J Secombes, M Mazzini, G Scapigliati

Research output: Contribution to journal › Article

51 Citations (Scopus)

Abstract

Biological activities of a putative mature sea bass interleukin-1 beta peptide, produced as a recombinant protein (rIL-1 beta) in Escherichia coli, have been investigated. The rIL-1 beta contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1 beta induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1 beta administered intraperitoneally increased expression levels of the IL-1 beta gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.

Original language	English
Pages (from-to)	609-617
Number of pages	9
Journal	Marine Biotechnology
Volume	7
DOIs	https://doi.org/10.1007/s10126-004-5131-5
Publication status	Published - 2005

Keywords

European sea bass
cytokine
interleukin 1 beta
proliferation
phagocytosis
cyclooxygenase 2
TROUT ONCORHYNCHUS-MYKISS
RAINBOW-TROUT
EXPRESSION ANALYSIS
MOLECULAR-CLONING
RECEPTOR
IL-1-BETA
FISH
CARP
MACROPHAGES
ACTIVATION

Access to Document

10.1007/s10126-004-5131-5

Cite this

@article{b9a3901de0764ed4a671b33e3bfcc3af,

title = "Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1 beta",

abstract = "Biological activities of a putative mature sea bass interleukin-1 beta peptide, produced as a recombinant protein (rIL-1 beta) in Escherichia coli, have been investigated. The rIL-1 beta contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1 beta induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1 beta administered intraperitoneally increased expression levels of the IL-1 beta gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.",

keywords = "European sea bass, cytokine, interleukin 1 beta, proliferation, phagocytosis, cyclooxygenase 2, TROUT ONCORHYNCHUS-MYKISS, RAINBOW-TROUT, EXPRESSION ANALYSIS, MOLECULAR-CLONING, RECEPTOR, IL-1-BETA, FISH, CARP, MACROPHAGES, ACTIVATION",

author = "F Buonocore and M Forlenza and E Randelli and S Benedetti and P Bossu and S Meloni and Secombes, {C J} and M Mazzini and G Scapigliati",

year = "2005",

doi = "10.1007/s10126-004-5131-5",

language = "English",

volume = "7",

pages = "609--617",

journal = "Marine Biotechnology",

issn = "1436-2228",

publisher = "Springer Verlag",

}

TY - JOUR

T1 - Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1 beta

AU - Buonocore, F

AU - Forlenza, M

AU - Randelli, E

AU - Benedetti, S

AU - Bossu, P

AU - Meloni, S

AU - Secombes, C J

AU - Mazzini, M

AU - Scapigliati, G

PY - 2005

Y1 - 2005

N2 - Biological activities of a putative mature sea bass interleukin-1 beta peptide, produced as a recombinant protein (rIL-1 beta) in Escherichia coli, have been investigated. The rIL-1 beta contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1 beta induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1 beta administered intraperitoneally increased expression levels of the IL-1 beta gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.

AB - Biological activities of a putative mature sea bass interleukin-1 beta peptide, produced as a recombinant protein (rIL-1 beta) in Escherichia coli, have been investigated. The rIL-1 beta contains a 6-histidine tag at the N-terminus, and protein purification has been achieved through this tag by affinity chromatography. Biological activities have been investigated both at the cellular and gene expression levels. In in vitro assays sea bass rIL-1 beta induced the proliferation of murine D10.G4.1 cells and increased yeast phagocytosis by sea bass head kidney leukocytes. The purified cytokine was also tested in a lymphocyte-activation factor assay, where it induced the proliferation of sea bass thymocytes. Finally, in an in vivo assay, rIL-1 beta administered intraperitoneally increased expression levels of the IL-1 beta gene and activated macrophages to produce a cyclooxygenase 2 homologue (COX-2) gene in the head kidney.

KW - European sea bass

KW - cytokine

KW - interleukin 1 beta

KW - proliferation

KW - phagocytosis

KW - cyclooxygenase 2

KW - TROUT ONCORHYNCHUS-MYKISS

KW - RAINBOW-TROUT

KW - EXPRESSION ANALYSIS

KW - MOLECULAR-CLONING

KW - RECEPTOR

KW - IL-1-BETA

KW - FISH

KW - CARP

KW - MACROPHAGES

KW - ACTIVATION

U2 - 10.1007/s10126-004-5131-5

DO - 10.1007/s10126-004-5131-5

M3 - Article

SN - 1436-2228

VL - 7

SP - 609

EP - 617

JO - Marine Biotechnology

JF - Marine Biotechnology

ER -

Biological activity of sea bass (Dicentrarchus labrax L.) recombinant interleukin-1 beta

Abstract

Keywords

Access to Document

Fingerprint

Cite this