Biomarkers of caspofungin resistance in Candida albicans isolates: A proteomic approach

Giuseppe Buda de Cesare, Ahmed Hafez, David Stead, Carlos Llorens, Carol Munro

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Candida albicans is a clinically important polymorphic fungal pathogen that causes lifethreatening invasive infections in immunocompromised patients. Antifungal therapy failure is 10 a substantial clinical problem, due to the emergence of an increasing number ofdrug-resistant isolates. Caspofungin is a common antifungal drug, often used as first-line therapy that inhibits cell wall β-(1,3)-glucan synthesis. In this work, the cell surface of different echinocandin-resistant C. albicans clinical isolates was compared with sensitive isolates and their responses to echinocandin treatment analyzed. Proteomic analysis detected changes in the repertoire of proteins 15 involved in cell wall organization and maintenance, in drug-resistant strains compared to susceptible isolates and after incubation with caspofungin. Moreover, an interaction network was created from the differential expression results. Our findings suggest drug resistance may involve not only a different cell wall architecture, but also a different response to drugs.
Original languageEnglish
JournalVirulence
DOIs
Publication statusAccepted/In press - 19 May 2022

Keywords

  • Candida albicans
  • drug resistance
  • caspofungin
  • proteomics
  • interaction network

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