Biosensing 2,4-dichlorophenol toxicity during biodegradation by Burkholderia sp RASC c2 in soil

Y Beaton, L J Shaw, L A Glover, A A Meharg, K Killham

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Biodegradation of the model pollutant, 2,4-dichiorophenol (2,4-DCP) by Burkholderia sp. RASC c2, in contaminated sail was assessed by combining chemical analysis with a toxicity test using Escherichia coli HB101 pUCD607. E coli HB101 pUCD607 was previously marked with luxCDABE genes, encoding bacterial bioluminescence and was used as an alternative to Microtox. Mineralization of C-14-2,4-DCP (196.2 mu g g(-1) dry wt) in soil occurred rapidly after a 24 h lag. Correspondingly, 2,4-DCP concentrations in soil and soil water extra cts decreased with time and concentrations in the latter were at background levels (<0.12 mu g mL(-1)) after day 2. Toxicity of soil water extracts to the lux-based biosensor also decreased with time. Mean light output of E. coli was stimulated by similar to 1.5 X control values in sail wafer extracts when concentrations of 2,4-DCP were approaching the limit of detection by HPLC but returned to, values equivalent to those of controls when soil water 2,4-DCP concentrations were below the detection limit. No mineralization or microbial growth was detected in noninoculated microcosms. 2,4-DCP concentration in sterile controls decreased significantly with time as did toxicity to E. colt Lux-based E, coli was a sensitive biosensor of 2,4-DCP toxicity during biodegradation and results complemented chemical analysis.

Original languageEnglish
Pages (from-to)4086-4091
Number of pages6
JournalEnvironmental Science & Technology
Volume33
Publication statusPublished - 1999

Keywords

  • LUX-MODIFIED BACTERIAL
  • BIOREMEDIATION
  • TESTS
  • CHLOROPHENOLS
  • DEGRADATION
  • CHEMICALS
  • CATECHOL
  • BINDING
  • 2,4-D
  • FATE

Cite this

Beaton, Y., Shaw, L. J., Glover, L. A., Meharg, A. A., & Killham, K. (1999). Biosensing 2,4-dichlorophenol toxicity during biodegradation by Burkholderia sp RASC c2 in soil. Environmental Science & Technology, 33, 4086-4091.

Biosensing 2,4-dichlorophenol toxicity during biodegradation by Burkholderia sp RASC c2 in soil. / Beaton, Y ; Shaw, L J ; Glover, L A ; Meharg, A A ; Killham, K .

In: Environmental Science & Technology, Vol. 33, 1999, p. 4086-4091.

Research output: Contribution to journalArticle

Beaton, Y, Shaw, LJ, Glover, LA, Meharg, AA & Killham, K 1999, 'Biosensing 2,4-dichlorophenol toxicity during biodegradation by Burkholderia sp RASC c2 in soil' Environmental Science & Technology, vol. 33, pp. 4086-4091.
Beaton, Y ; Shaw, L J ; Glover, L A ; Meharg, A A ; Killham, K . / Biosensing 2,4-dichlorophenol toxicity during biodegradation by Burkholderia sp RASC c2 in soil. In: Environmental Science & Technology. 1999 ; Vol. 33. pp. 4086-4091.
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AB - Biodegradation of the model pollutant, 2,4-dichiorophenol (2,4-DCP) by Burkholderia sp. RASC c2, in contaminated sail was assessed by combining chemical analysis with a toxicity test using Escherichia coli HB101 pUCD607. E coli HB101 pUCD607 was previously marked with luxCDABE genes, encoding bacterial bioluminescence and was used as an alternative to Microtox. Mineralization of C-14-2,4-DCP (196.2 mu g g(-1) dry wt) in soil occurred rapidly after a 24 h lag. Correspondingly, 2,4-DCP concentrations in soil and soil water extra cts decreased with time and concentrations in the latter were at background levels (<0.12 mu g mL(-1)) after day 2. Toxicity of soil water extracts to the lux-based biosensor also decreased with time. Mean light output of E. coli was stimulated by similar to 1.5 X control values in sail wafer extracts when concentrations of 2,4-DCP were approaching the limit of detection by HPLC but returned to, values equivalent to those of controls when soil water 2,4-DCP concentrations were below the detection limit. No mineralization or microbial growth was detected in noninoculated microcosms. 2,4-DCP concentration in sterile controls decreased significantly with time as did toxicity to E. colt Lux-based E, coli was a sensitive biosensor of 2,4-DCP toxicity during biodegradation and results complemented chemical analysis.

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