Biosensing the acute toxicity of metal interactions: Are they additive, synergistic, or antagonistic?

S Preston, N Coad, J Townend, K Killham, G I Paton

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

The toxicity of Zn, Cu, and Cd, alone or in combination, was assessed using two luminescence-based microbial biosensors from different ecological niches: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586 pUCD607. Significant synergistic interactions occurred between the toxic effects of the Zn and Cu and Zn and Cd combinations and the response of E. coli (i.e., toxicities of combinations of pollutants were greater than predicted from addition of individual toxicities). Significant synergistic interactions were also observed between the toxicities of Cd and Cu combinations and the response of P. fluorescens. A longer exposure time was shown to significantly increase the sensitivity of E. coli to the metal pollutants. P. fluorescens was observed to significantly decrease in its sensitivity toward Zn and Cd with a longer exposure time. It was shown that the toxicity of combinations of metals could not be modeled on the basis that their toxic action was independent of each other. The application of different models to describe interactions between combinations of metals is discussed. The importance of considering the test species and the exposure period selected for toxicity assessment was highlighted, as was the need to further investigate the toxicity of combinations of pollutants.

Original languageEnglish
Pages (from-to)775-780
Number of pages6
JournalEnvironmental Toxicology and Chemistry
Volume19
Publication statusPublished - 2000

Keywords

  • heavy metals
  • pollutant interactions
  • microbial biosensors
  • toxicity testing
  • bioavailability
  • PIGMENTED-SALMON-SYNDROME
  • BIOLUMINESCENCE
  • CONTAMINANTS
  • BACTERIA
  • MIXTURES
  • CADMIUM
  • TESTS
  • IONS
  • SOIL

Cite this

Biosensing the acute toxicity of metal interactions: Are they additive, synergistic, or antagonistic? / Preston, S ; Coad, N ; Townend, J ; Killham, K ; Paton, G I .

In: Environmental Toxicology and Chemistry, Vol. 19, 2000, p. 775-780.

Research output: Contribution to journalArticle

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abstract = "The toxicity of Zn, Cu, and Cd, alone or in combination, was assessed using two luminescence-based microbial biosensors from different ecological niches: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586 pUCD607. Significant synergistic interactions occurred between the toxic effects of the Zn and Cu and Zn and Cd combinations and the response of E. coli (i.e., toxicities of combinations of pollutants were greater than predicted from addition of individual toxicities). Significant synergistic interactions were also observed between the toxicities of Cd and Cu combinations and the response of P. fluorescens. A longer exposure time was shown to significantly increase the sensitivity of E. coli to the metal pollutants. P. fluorescens was observed to significantly decrease in its sensitivity toward Zn and Cd with a longer exposure time. It was shown that the toxicity of combinations of metals could not be modeled on the basis that their toxic action was independent of each other. The application of different models to describe interactions between combinations of metals is discussed. The importance of considering the test species and the exposure period selected for toxicity assessment was highlighted, as was the need to further investigate the toxicity of combinations of pollutants.",
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T1 - Biosensing the acute toxicity of metal interactions: Are they additive, synergistic, or antagonistic?

AU - Preston, S

AU - Coad, N

AU - Townend, J

AU - Killham, K

AU - Paton, G I

PY - 2000

Y1 - 2000

N2 - The toxicity of Zn, Cu, and Cd, alone or in combination, was assessed using two luminescence-based microbial biosensors from different ecological niches: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586 pUCD607. Significant synergistic interactions occurred between the toxic effects of the Zn and Cu and Zn and Cd combinations and the response of E. coli (i.e., toxicities of combinations of pollutants were greater than predicted from addition of individual toxicities). Significant synergistic interactions were also observed between the toxicities of Cd and Cu combinations and the response of P. fluorescens. A longer exposure time was shown to significantly increase the sensitivity of E. coli to the metal pollutants. P. fluorescens was observed to significantly decrease in its sensitivity toward Zn and Cd with a longer exposure time. It was shown that the toxicity of combinations of metals could not be modeled on the basis that their toxic action was independent of each other. The application of different models to describe interactions between combinations of metals is discussed. The importance of considering the test species and the exposure period selected for toxicity assessment was highlighted, as was the need to further investigate the toxicity of combinations of pollutants.

AB - The toxicity of Zn, Cu, and Cd, alone or in combination, was assessed using two luminescence-based microbial biosensors from different ecological niches: Escherichia coli HB101 pUCD607 and Pseudomonas fluorescens 10586 pUCD607. Significant synergistic interactions occurred between the toxic effects of the Zn and Cu and Zn and Cd combinations and the response of E. coli (i.e., toxicities of combinations of pollutants were greater than predicted from addition of individual toxicities). Significant synergistic interactions were also observed between the toxicities of Cd and Cu combinations and the response of P. fluorescens. A longer exposure time was shown to significantly increase the sensitivity of E. coli to the metal pollutants. P. fluorescens was observed to significantly decrease in its sensitivity toward Zn and Cd with a longer exposure time. It was shown that the toxicity of combinations of metals could not be modeled on the basis that their toxic action was independent of each other. The application of different models to describe interactions between combinations of metals is discussed. The importance of considering the test species and the exposure period selected for toxicity assessment was highlighted, as was the need to further investigate the toxicity of combinations of pollutants.

KW - heavy metals

KW - pollutant interactions

KW - microbial biosensors

KW - toxicity testing

KW - bioavailability

KW - PIGMENTED-SALMON-SYNDROME

KW - BIOLUMINESCENCE

KW - CONTAMINANTS

KW - BACTERIA

KW - MIXTURES

KW - CADMIUM

KW - TESTS

KW - IONS

KW - SOIL

M3 - Article

VL - 19

SP - 775

EP - 780

JO - Environmental Toxicology and Chemistry

JF - Environmental Toxicology and Chemistry

SN - 0730-7268

ER -