Biosensor-based diagnostics of contaminated groundwater: assessment and remediation strategy.

J. Bhattacharyya, David Read, S. Amos, S. Dooley, Kenneth Stuart Killham, Graeme Iain Paton

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Shallow groundwater beneath a former airfield site in southern England has been heavily contaminated with a wide range of chlorinated solvents. The feasibility of using bacterial biosensors to complement chemical analysis and enable cost-effective, and focussed sampling has been assessed as part of a site evaluation programme. Five different biosensors, three metabolic (Vibrio fischeri, Pseudomonas fluorescens 10568 and Escherichia coli HB101) and two catabolic (Pseudomonas putida TVA8 and E. coli DH5alpha), were employed to identify areas where the availability and toxicity of pollutants is of most immediate environmental concern. The biosensors used showed different sensitivities to each other and to the groundwater samples tested. There was generally a good agreement with chemical analyses. The potential efficacy of remediation strategies was explored by coupling sample manipulation to biosensor tests. Manipulation involved sparging and charcoal treatment procedures to simulate remediative engineering solutions. Sparging was sufficient at most locations. (C) 2004 Elsevier Ltd. All rights reserved.

Original languageEnglish
Pages (from-to)485-492
Number of pages7
JournalEnvironmental Pollution
Volume134
DOIs
Publication statusPublished - 2005

Keywords

  • biosensor
  • luminescence
  • TCE
  • DNAPL
  • toxicity
  • PSEUDOMONAS-FLUORESCENS
  • TOXICITY ASSESSMENT
  • TRICHLOROETHYLENE
  • BIOREMEDIATION
  • WATER
  • HYDROCARBONS
  • METABOLISM
  • ADSORPTION
  • POLLUTANTS
  • BACTERIAL

Cite this

Biosensor-based diagnostics of contaminated groundwater: assessment and remediation strategy. / Bhattacharyya, J.; Read, David; Amos, S.; Dooley, S.; Killham, Kenneth Stuart; Paton, Graeme Iain.

In: Environmental Pollution, Vol. 134, 2005, p. 485-492.

Research output: Contribution to journalArticle

Bhattacharyya, J. ; Read, David ; Amos, S. ; Dooley, S. ; Killham, Kenneth Stuart ; Paton, Graeme Iain. / Biosensor-based diagnostics of contaminated groundwater: assessment and remediation strategy. In: Environmental Pollution. 2005 ; Vol. 134. pp. 485-492.
@article{4ad31969499b44cbb81e960bf96ca4b1,
title = "Biosensor-based diagnostics of contaminated groundwater: assessment and remediation strategy.",
abstract = "Shallow groundwater beneath a former airfield site in southern England has been heavily contaminated with a wide range of chlorinated solvents. The feasibility of using bacterial biosensors to complement chemical analysis and enable cost-effective, and focussed sampling has been assessed as part of a site evaluation programme. Five different biosensors, three metabolic (Vibrio fischeri, Pseudomonas fluorescens 10568 and Escherichia coli HB101) and two catabolic (Pseudomonas putida TVA8 and E. coli DH5alpha), were employed to identify areas where the availability and toxicity of pollutants is of most immediate environmental concern. The biosensors used showed different sensitivities to each other and to the groundwater samples tested. There was generally a good agreement with chemical analyses. The potential efficacy of remediation strategies was explored by coupling sample manipulation to biosensor tests. Manipulation involved sparging and charcoal treatment procedures to simulate remediative engineering solutions. Sparging was sufficient at most locations. (C) 2004 Elsevier Ltd. All rights reserved.",
keywords = "biosensor, luminescence, TCE, DNAPL, toxicity, PSEUDOMONAS-FLUORESCENS, TOXICITY ASSESSMENT, TRICHLOROETHYLENE, BIOREMEDIATION, WATER, HYDROCARBONS, METABOLISM, ADSORPTION, POLLUTANTS, BACTERIAL",
author = "J. Bhattacharyya and David Read and S. Amos and S. Dooley and Killham, {Kenneth Stuart} and Paton, {Graeme Iain}",
year = "2005",
doi = "10.1016/j.envpol.2004.09.002",
language = "English",
volume = "134",
pages = "485--492",
journal = "Environmental Pollution",
issn = "0269-7491",
publisher = "ELSEVIER APPL SCI PUBL LTD",

}

TY - JOUR

T1 - Biosensor-based diagnostics of contaminated groundwater: assessment and remediation strategy.

AU - Bhattacharyya, J.

AU - Read, David

AU - Amos, S.

AU - Dooley, S.

AU - Killham, Kenneth Stuart

AU - Paton, Graeme Iain

PY - 2005

Y1 - 2005

N2 - Shallow groundwater beneath a former airfield site in southern England has been heavily contaminated with a wide range of chlorinated solvents. The feasibility of using bacterial biosensors to complement chemical analysis and enable cost-effective, and focussed sampling has been assessed as part of a site evaluation programme. Five different biosensors, three metabolic (Vibrio fischeri, Pseudomonas fluorescens 10568 and Escherichia coli HB101) and two catabolic (Pseudomonas putida TVA8 and E. coli DH5alpha), were employed to identify areas where the availability and toxicity of pollutants is of most immediate environmental concern. The biosensors used showed different sensitivities to each other and to the groundwater samples tested. There was generally a good agreement with chemical analyses. The potential efficacy of remediation strategies was explored by coupling sample manipulation to biosensor tests. Manipulation involved sparging and charcoal treatment procedures to simulate remediative engineering solutions. Sparging was sufficient at most locations. (C) 2004 Elsevier Ltd. All rights reserved.

AB - Shallow groundwater beneath a former airfield site in southern England has been heavily contaminated with a wide range of chlorinated solvents. The feasibility of using bacterial biosensors to complement chemical analysis and enable cost-effective, and focussed sampling has been assessed as part of a site evaluation programme. Five different biosensors, three metabolic (Vibrio fischeri, Pseudomonas fluorescens 10568 and Escherichia coli HB101) and two catabolic (Pseudomonas putida TVA8 and E. coli DH5alpha), were employed to identify areas where the availability and toxicity of pollutants is of most immediate environmental concern. The biosensors used showed different sensitivities to each other and to the groundwater samples tested. There was generally a good agreement with chemical analyses. The potential efficacy of remediation strategies was explored by coupling sample manipulation to biosensor tests. Manipulation involved sparging and charcoal treatment procedures to simulate remediative engineering solutions. Sparging was sufficient at most locations. (C) 2004 Elsevier Ltd. All rights reserved.

KW - biosensor

KW - luminescence

KW - TCE

KW - DNAPL

KW - toxicity

KW - PSEUDOMONAS-FLUORESCENS

KW - TOXICITY ASSESSMENT

KW - TRICHLOROETHYLENE

KW - BIOREMEDIATION

KW - WATER

KW - HYDROCARBONS

KW - METABOLISM

KW - ADSORPTION

KW - POLLUTANTS

KW - BACTERIAL

U2 - 10.1016/j.envpol.2004.09.002

DO - 10.1016/j.envpol.2004.09.002

M3 - Article

VL - 134

SP - 485

EP - 492

JO - Environmental Pollution

JF - Environmental Pollution

SN - 0269-7491

ER -