Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

Wael M Abdel-Mageed, Lamya H Al-Wahaibi, Burhan Lehri, Muneera S M Al-Saleem, Michael Goodfellow, Ali B Kusuma, Imen Nouioui, Hariadi Soleh, Wasu Pathom-Aree, Marcel Jaspars, Andrey V Karlyshev

Research output: Contribution to journalArticlepeer-review

Abstract

A Micromonospora strain, isolate MT25T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1H-15N HSQC and 1H-15N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25T (NCIMB 15245T, TISTR 2834T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.

Original languageEnglish
Article number243
Number of pages19
JournalMarine Drugs
Volume19
Issue number5
DOIs
Publication statusPublished - 25 Apr 2021

Keywords

  • Mariana Trench
  • Micromonospora provocatoris MT25
  • desferrioxamine
  • n-acetylglutaminyl glutamine amide
  • 1H-15N 2D-NMR
  • genomics
  • biosynthetic gene clusters
  • stress genes

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