Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

Wael M Abdel-Mageed; Lamya H Al-Wahaibi; Burhan Lehri; Muneera S M Al-Saleem; Michael Goodfellow; Ali B Kusuma; Imen Nouioui; Hariadi Soleh; Wasu Pathom-Aree; Marcel Jaspars; Andrey V Karlyshev

doi:10.3390/md19050243

Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

Wael M Abdel-Mageed, Lamya H Al-Wahaibi, Burhan Lehri, Muneera S M Al-Saleem, Michael Goodfellow, Ali B Kusuma, Imen Nouioui, Hariadi Soleh, Wasu Pathom-Aree, Marcel Jaspars, Andrey V Karlyshev

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Abstract

A Micromonospora strain, isolate MT25T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1H-15N HSQC and 1H-15N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25T (NCIMB 15245T, TISTR 2834T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.

Original language	English
Article number	243
Number of pages	19
Journal	Marine Drugs
Volume	19
Issue number	5
DOIs	https://doi.org/10.3390/md19050243
Publication status	Published - 25 Apr 2021

Bibliographical note

Acknowledgments
This work was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1440-0014) (2). M.G. is indebted to Jose M. Iqbal (Instituto de Resoursos Naturales Y Agrobilogia de Salamanca, Spain) for undertaking the fatty acid analyses. We thank Anthony Wright for acquiring the NMR data at the Australian Institute of Marine Sciences.

Funding: This work was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1440-0014) (2).

Keywords

Mariana Trench
Micromonospora provocatoris MT25
desferrioxamine
n-acetylglutaminyl glutamine amide
1H-15N 2D-NMR
genomics
biosynthetic gene clusters
stress genes

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Abdel-Mageed, W. M., Al-Wahaibi, L. H., Lehri, B., Al-Saleem, M. S. M., Goodfellow, M., Kusuma, A. B., Nouioui, I., Soleh, H., Pathom-Aree, W., Jaspars, M., & Karlyshev, A. V. (2021). Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench. Marine Drugs, 19(5), Article 243. https://doi.org/10.3390/md19050243

Abdel-Mageed, WM, Al-Wahaibi, LH, Lehri, B, Al-Saleem, MSM, Goodfellow, M, Kusuma, AB, Nouioui, I, Soleh, H, Pathom-Aree, W, Jaspars, M & Karlyshev, AV 2021, 'Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench', Marine Drugs, vol. 19, no. 5, 243. https://doi.org/10.3390/md19050243

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title = "Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench",

abstract = "A Micromonospora strain, isolate MT25T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1H-15N HSQC and 1H-15N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25T (NCIMB 15245T, TISTR 2834T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.",

keywords = "Mariana Trench, Micromonospora provocatoris MT25, desferrioxamine, n-acetylglutaminyl glutamine amide, 1H-15N 2D-NMR, genomics, biosynthetic gene clusters, stress genes",

author = "Abdel-Mageed, {Wael M} and Al-Wahaibi, {Lamya H} and Burhan Lehri and Al-Saleem, {Muneera S M} and Michael Goodfellow and Kusuma, {Ali B} and Imen Nouioui and Hariadi Soleh and Wasu Pathom-Aree and Marcel Jaspars and Karlyshev, {Andrey V}",

note = "Acknowledgments This work was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1440-0014) (2). M.G. is indebted to Jose M. Iqbal (Instituto de Resoursos Naturales Y Agrobilogia de Salamanca, Spain) for undertaking the fatty acid analyses. We thank Anthony Wright for acquiring the NMR data at the Australian Institute of Marine Sciences. Funding: This work was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1440-0014) (2).",

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AU - Abdel-Mageed, Wael M

AU - Al-Wahaibi, Lamya H

AU - Lehri, Burhan

AU - Al-Saleem, Muneera S M

AU - Goodfellow, Michael

AU - Kusuma, Ali B

AU - Nouioui, Imen

AU - Soleh, Hariadi

AU - Pathom-Aree, Wasu

AU - Jaspars, Marcel

AU - Karlyshev, Andrey V

N1 - Acknowledgments This work was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1440-0014) (2). M.G. is indebted to Jose M. Iqbal (Instituto de Resoursos Naturales Y Agrobilogia de Salamanca, Spain) for undertaking the fatty acid analyses. We thank Anthony Wright for acquiring the NMR data at the Australian Institute of Marine Sciences. Funding: This work was funded by the Deanship of Scientific Research at Princess Nourah bint Abdulrahman University, through the Research Groups Program Grant no. (RGP-1440-0014) (2).

PY - 2021/4/25

Y1 - 2021/4/25

N2 - A Micromonospora strain, isolate MT25T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1H-15N HSQC and 1H-15N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25T (NCIMB 15245T, TISTR 2834T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.

AB - A Micromonospora strain, isolate MT25T, was recovered from a sediment collected from the Challenger Deep of the Mariana Trench using a selective isolation procedure. The isolate produced two major metabolites, n-acetylglutaminyl glutamine amide and desferrioxamine B, the chemical structures of which were determined using 1D and 2D-NMR, including 1H-15N HSQC and 1H-15N HMBC 2D-NMR, as well as high resolution MS. A whole genome sequence of the strain showed the presence of ten natural product-biosynthetic gene clusters, including one responsible for the biosynthesis of desferrioxamine B. Whilst 16S rRNA gene sequence analyses showed that the isolate was most closely related to the type strain of Micromonospora chalcea, a whole genome sequence analysis revealed it to be most closely related to Micromonospora tulbaghiae 45142T. The two strains were distinguished using a combination of genomic and phenotypic features. Based on these data, it is proposed that strain MT25T (NCIMB 15245T, TISTR 2834T) be classified as Micromonospora provocatoris sp. nov. Analysis of the genome sequence of strain MT25T (genome size 6.1 Mbp) revealed genes predicted to responsible for its adaptation to extreme environmental conditions that prevail in deep-sea sediments.

KW - Mariana Trench

KW - Micromonospora provocatoris MT25

KW - desferrioxamine

KW - n-acetylglutaminyl glutamine amide

KW - 1H-15N 2D-NMR

KW - genomics

KW - biosynthetic gene clusters

KW - stress genes

U2 - 10.3390/md19050243

DO - 10.3390/md19050243

M3 - Article

C2 - 33923039

SN - 1660-3397

VL - 19

JO - Marine Drugs

JF - Marine Drugs

IS - 5

M1 - 243

ER -

Biotechnological and Ecological Potential of Micromonospora provocatoris sp. nov., a Gifted Strain Isolated from the Challenger Deep of the Mariana Trench

Abstract

Bibliographical note

Keywords

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