Biotransformation of arsenate to arsenosugars by Chlorella vulgaris

L. A. Murray, Andrea Raab, Iain J Marr, Jorg Feldmann

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Chlorella vulgaris was cultivated in a growth medium containing arsenate concentration of <0.01, 10, 100 and 1000 mg 1(-1). Illumination was carried out in 12 h cycles for 5 days. The health status of the culture was monitored by continuous pH and dissolved oxygen (DO) readings. Destructive sampling was used for the determination of biomass, chlorophyll, total arsenic and arsenic species. The chlorophyll a content, the DO and pH cycles were not significantly different for the different arsenate concentrations in the culture. In contrast, biomass production was significantly (p < 0.05) increased for the arsenic(V) treatment at 1000 mg 1(-1) compared with 100 mg 1(-1).

The arsenic concentration in the algae increased with the arsenate concentration in the culture. However, the bioconcentration factor decreased a hundred-fold with increase of arsenate from the background level to 1000 mg 1(-1). The arsenic species were identified by using strong anion-exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry analysis after methanol/water (1:1) extraction. The majority (87-100%) of the extractable arsenic was still arsenate; arsenite was found to be between 1 and 6% of total extractable arsenic in the algae. In addition to dimethylarsinic acid, one unknown arsenical (almost co-eluting with methylarsonic acid) and three different arsenosugars have been identified for the first time in C. vulgaris growing in a culture containing a mixture of antibiotics and believed to be axenic. The transformation to arsenosugars in the algae is not dependent on the arsenate concentration in the culture and varies between 0.2 and 5% of total accumulated arsenic. Although no microbiological tests for bacterial contamination were made, this study supports the hypothesis that algae, and not associated bacteria, produce the arsenosugars. Copyright (C) 2003 John Wiley Sons, Ltd.

Original languageEnglish
Pages (from-to)669-674
Number of pages5
JournalApplied Organometallic Chemistry
Volume17
Issue number17
DOIs
Publication statusPublished - 2003

Keywords

  • arsenic
  • bioaccumulation
  • alga
  • Chlorella vulgaris
  • arsenosugars
  • ALGA FUCUS-GARDNERI
  • SEAWEED
  • BIOTRANSFORMATION
  • ARSENOBETAINE
  • ENVIRONMENT
  • ARSENIC(V)
  • METABOLISM
  • EXCRETION
  • SHEEP

Cite this

Biotransformation of arsenate to arsenosugars by Chlorella vulgaris. / Murray, L. A.; Raab, Andrea; Marr, Iain J; Feldmann, Jorg.

In: Applied Organometallic Chemistry, Vol. 17, No. 17, 2003, p. 669-674.

Research output: Contribution to journalArticle

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abstract = "Chlorella vulgaris was cultivated in a growth medium containing arsenate concentration of <0.01, 10, 100 and 1000 mg 1(-1). Illumination was carried out in 12 h cycles for 5 days. The health status of the culture was monitored by continuous pH and dissolved oxygen (DO) readings. Destructive sampling was used for the determination of biomass, chlorophyll, total arsenic and arsenic species. The chlorophyll a content, the DO and pH cycles were not significantly different for the different arsenate concentrations in the culture. In contrast, biomass production was significantly (p < 0.05) increased for the arsenic(V) treatment at 1000 mg 1(-1) compared with 100 mg 1(-1).The arsenic concentration in the algae increased with the arsenate concentration in the culture. However, the bioconcentration factor decreased a hundred-fold with increase of arsenate from the background level to 1000 mg 1(-1). The arsenic species were identified by using strong anion-exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry analysis after methanol/water (1:1) extraction. The majority (87-100{\%}) of the extractable arsenic was still arsenate; arsenite was found to be between 1 and 6{\%} of total extractable arsenic in the algae. In addition to dimethylarsinic acid, one unknown arsenical (almost co-eluting with methylarsonic acid) and three different arsenosugars have been identified for the first time in C. vulgaris growing in a culture containing a mixture of antibiotics and believed to be axenic. The transformation to arsenosugars in the algae is not dependent on the arsenate concentration in the culture and varies between 0.2 and 5{\%} of total accumulated arsenic. Although no microbiological tests for bacterial contamination were made, this study supports the hypothesis that algae, and not associated bacteria, produce the arsenosugars. Copyright (C) 2003 John Wiley Sons, Ltd.",
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AU - Murray, L. A.

AU - Raab, Andrea

AU - Marr, Iain J

AU - Feldmann, Jorg

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N2 - Chlorella vulgaris was cultivated in a growth medium containing arsenate concentration of <0.01, 10, 100 and 1000 mg 1(-1). Illumination was carried out in 12 h cycles for 5 days. The health status of the culture was monitored by continuous pH and dissolved oxygen (DO) readings. Destructive sampling was used for the determination of biomass, chlorophyll, total arsenic and arsenic species. The chlorophyll a content, the DO and pH cycles were not significantly different for the different arsenate concentrations in the culture. In contrast, biomass production was significantly (p < 0.05) increased for the arsenic(V) treatment at 1000 mg 1(-1) compared with 100 mg 1(-1).The arsenic concentration in the algae increased with the arsenate concentration in the culture. However, the bioconcentration factor decreased a hundred-fold with increase of arsenate from the background level to 1000 mg 1(-1). The arsenic species were identified by using strong anion-exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry analysis after methanol/water (1:1) extraction. The majority (87-100%) of the extractable arsenic was still arsenate; arsenite was found to be between 1 and 6% of total extractable arsenic in the algae. In addition to dimethylarsinic acid, one unknown arsenical (almost co-eluting with methylarsonic acid) and three different arsenosugars have been identified for the first time in C. vulgaris growing in a culture containing a mixture of antibiotics and believed to be axenic. The transformation to arsenosugars in the algae is not dependent on the arsenate concentration in the culture and varies between 0.2 and 5% of total accumulated arsenic. Although no microbiological tests for bacterial contamination were made, this study supports the hypothesis that algae, and not associated bacteria, produce the arsenosugars. Copyright (C) 2003 John Wiley Sons, Ltd.

AB - Chlorella vulgaris was cultivated in a growth medium containing arsenate concentration of <0.01, 10, 100 and 1000 mg 1(-1). Illumination was carried out in 12 h cycles for 5 days. The health status of the culture was monitored by continuous pH and dissolved oxygen (DO) readings. Destructive sampling was used for the determination of biomass, chlorophyll, total arsenic and arsenic species. The chlorophyll a content, the DO and pH cycles were not significantly different for the different arsenate concentrations in the culture. In contrast, biomass production was significantly (p < 0.05) increased for the arsenic(V) treatment at 1000 mg 1(-1) compared with 100 mg 1(-1).The arsenic concentration in the algae increased with the arsenate concentration in the culture. However, the bioconcentration factor decreased a hundred-fold with increase of arsenate from the background level to 1000 mg 1(-1). The arsenic species were identified by using strong anion-exchange high-performance liquid chromatography-inductively coupled plasma mass spectrometry analysis after methanol/water (1:1) extraction. The majority (87-100%) of the extractable arsenic was still arsenate; arsenite was found to be between 1 and 6% of total extractable arsenic in the algae. In addition to dimethylarsinic acid, one unknown arsenical (almost co-eluting with methylarsonic acid) and three different arsenosugars have been identified for the first time in C. vulgaris growing in a culture containing a mixture of antibiotics and believed to be axenic. The transformation to arsenosugars in the algae is not dependent on the arsenate concentration in the culture and varies between 0.2 and 5% of total accumulated arsenic. Although no microbiological tests for bacterial contamination were made, this study supports the hypothesis that algae, and not associated bacteria, produce the arsenosugars. Copyright (C) 2003 John Wiley Sons, Ltd.

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KW - bioaccumulation

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KW - ALGA FUCUS-GARDNERI

KW - SEAWEED

KW - BIOTRANSFORMATION

KW - ARSENOBETAINE

KW - ENVIRONMENT

KW - ARSENIC(V)

KW - METABOLISM

KW - EXCRETION

KW - SHEEP

U2 - 10.1002/aoc.498

DO - 10.1002/aoc.498

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VL - 17

SP - 669

EP - 674

JO - Applied Organometallic Chemistry

JF - Applied Organometallic Chemistry

SN - 0268-2605

IS - 17

ER -