Biphasic zinc compartmentalisation in a human fungal pathogen

Aaron C. Crawford, Laura E. Lehtovirta, Omran Alamir, Maria J. Niemiec, Bader Alawfi, Mohammad Alsarraf, Volha Skrahina, Anna C. B. P. Costa, Andrew Anderson, Sujan Yellagunda, Elizabeth Ballou, Bernhard Hube, Constantin F. Urban, Duncan Wilson

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Abstract

Nutritional immunity describes the host-driven manipulation of essential micronutrients, including iron, zinc and manganese. To withstand nutritional immunity and proliferate within their hosts, pathogenic microbes must express efficient micronutrient uptake and homeostatic systems. Here we have elucidated the pathway of cellularzinc assimilation in the major human fungal pathogen Candida albicans. Bioinformatics analysis identified nine putative zinc transporters: four cytoplasmic-import Zip proteins (Zrt1, Zrt2, Zrt3 and orf19.5428) and five cytoplasmic-export ZnT proteins (orf19.1536/Zrc1, orf19.3874, orf19.3769, orf19.3132 and orf19.52). Only Zrt1 and Zrt2 are predicted to localise to the plasma membrane and here we demonstrate that Zrt2 is essential for C. albicans zinc uptake and growth at acidic pH. In contrast, ZRT1 expression was found to be highly pH-dependent and could support growth of the ZRT2-null strain at pH 7 and above. This regulatory paradigm is analogous to the distantly related pathogenic mould, Aspergillus fumigatus, suggesting that pH-adaptation of zinc transport may be conserved in fungi and we propose that environmental pH has shaped the evolution of zinc import systems in fungi. Deletion of C. albicans ZRT2 reduced fungal burden in wild type, but not in mice lacking the zinc-chelating antimicrobial protein calprotectin. Inhibition of zrt2 growth by neutrophil extracellular traps was calprotectin-dependent. This suggests that, within the kidney, C. albicans growth is determined by pathogen-Zrt2 and host-calprotectin. As well as serving as an essential micronutrient, zinc can also be highly toxic and we show that C. albicans deals with this potential threat by rapidly compartmentalising zinc within vesicular stores called zincosomes. In order to understand mechanistically how this process occurs, we created deletion mutants of all five ZnT-type transporters in C. albicans. Here we show that, unlike in Saccharomyces cerevisiae, C. albicans Zrc1 mediates zinc tolerance via zincosomal zinc compartmentalisation. This novel transporter was also essential for virulence and liver colonisation in vivo. In summary, we show that zinc homeostasis in a major human fungal pathogen is a multi-stage process initiated by Zrt1/Zrt2-cellular import, followed by Zrc1-dependent intracellular compartmentalisation.
Original languageEnglish
Article numbere1007013
JournalPLoS Pathogens
Volume14
Issue number5
DOIs
Publication statusPublished - 4 May 2018

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Zinc
Candida albicans
Leukocyte L1 Antigen Complex
Micronutrients
Fungi
Growth
Immunity
Proteins
Aspergillus fumigatus
Poisons
Manganese
Computational Biology
Virulence
Saccharomyces cerevisiae
Homeostasis
Iron
Cell Membrane
Kidney
Liver

Cite this

Crawford, A. C., Lehtovirta, L. E., Alamir, O., Niemiec, M. J., Alawfi, B., Alsarraf, M., ... Wilson, D. (2018). Biphasic zinc compartmentalisation in a human fungal pathogen. PLoS Pathogens, 14(5), [e1007013]. https://doi.org/10.1371/journal.ppat.1007013

Biphasic zinc compartmentalisation in a human fungal pathogen. / Crawford, Aaron C.; Lehtovirta, Laura E.; Alamir, Omran; Niemiec, Maria J.; Alawfi, Bader; Alsarraf, Mohammad; Skrahina, Volha; Costa, Anna C. B. P.; Anderson, Andrew; Yellagunda, Sujan; Ballou, Elizabeth; Hube, Bernhard; Urban, Constantin F.; Wilson, Duncan.

In: PLoS Pathogens, Vol. 14, No. 5, e1007013, 04.05.2018.

Research output: Contribution to journalArticle

Crawford, AC, Lehtovirta, LE, Alamir, O, Niemiec, MJ, Alawfi, B, Alsarraf, M, Skrahina, V, Costa, ACBP, Anderson, A, Yellagunda, S, Ballou, E, Hube, B, Urban, CF & Wilson, D 2018, 'Biphasic zinc compartmentalisation in a human fungal pathogen', PLoS Pathogens, vol. 14, no. 5, e1007013. https://doi.org/10.1371/journal.ppat.1007013
Crawford AC, Lehtovirta LE, Alamir O, Niemiec MJ, Alawfi B, Alsarraf M et al. Biphasic zinc compartmentalisation in a human fungal pathogen. PLoS Pathogens. 2018 May 4;14(5). e1007013. https://doi.org/10.1371/journal.ppat.1007013
Crawford, Aaron C. ; Lehtovirta, Laura E. ; Alamir, Omran ; Niemiec, Maria J. ; Alawfi, Bader ; Alsarraf, Mohammad ; Skrahina, Volha ; Costa, Anna C. B. P. ; Anderson, Andrew ; Yellagunda, Sujan ; Ballou, Elizabeth ; Hube, Bernhard ; Urban, Constantin F. ; Wilson, Duncan. / Biphasic zinc compartmentalisation in a human fungal pathogen. In: PLoS Pathogens. 2018 ; Vol. 14, No. 5.
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abstract = "Nutritional immunity describes the host-driven manipulation of essential micronutrients, including iron, zinc and manganese. To withstand nutritional immunity and proliferate within their hosts, pathogenic microbes must express efficient micronutrient uptake and homeostatic systems. Here we have elucidated the pathway of cellularzinc assimilation in the major human fungal pathogen Candida albicans. Bioinformatics analysis identified nine putative zinc transporters: four cytoplasmic-import Zip proteins (Zrt1, Zrt2, Zrt3 and orf19.5428) and five cytoplasmic-export ZnT proteins (orf19.1536/Zrc1, orf19.3874, orf19.3769, orf19.3132 and orf19.52). Only Zrt1 and Zrt2 are predicted to localise to the plasma membrane and here we demonstrate that Zrt2 is essential for C. albicans zinc uptake and growth at acidic pH. In contrast, ZRT1 expression was found to be highly pH-dependent and could support growth of the ZRT2-null strain at pH 7 and above. This regulatory paradigm is analogous to the distantly related pathogenic mould, Aspergillus fumigatus, suggesting that pH-adaptation of zinc transport may be conserved in fungi and we propose that environmental pH has shaped the evolution of zinc import systems in fungi. Deletion of C. albicans ZRT2 reduced fungal burden in wild type, but not in mice lacking the zinc-chelating antimicrobial protein calprotectin. Inhibition of zrt2 growth by neutrophil extracellular traps was calprotectin-dependent. This suggests that, within the kidney, C. albicans growth is determined by pathogen-Zrt2 and host-calprotectin. As well as serving as an essential micronutrient, zinc can also be highly toxic and we show that C. albicans deals with this potential threat by rapidly compartmentalising zinc within vesicular stores called zincosomes. In order to understand mechanistically how this process occurs, we created deletion mutants of all five ZnT-type transporters in C. albicans. Here we show that, unlike in Saccharomyces cerevisiae, C. albicans Zrc1 mediates zinc tolerance via zincosomal zinc compartmentalisation. This novel transporter was also essential for virulence and liver colonisation in vivo. In summary, we show that zinc homeostasis in a major human fungal pathogen is a multi-stage process initiated by Zrt1/Zrt2-cellular import, followed by Zrc1-dependent intracellular compartmentalisation.",
author = "Crawford, {Aaron C.} and Lehtovirta, {Laura E.} and Omran Alamir and Niemiec, {Maria J.} and Bader Alawfi and Mohammad Alsarraf and Volha Skrahina and Costa, {Anna C. B. P.} and Andrew Anderson and Sujan Yellagunda and Elizabeth Ballou and Bernhard Hube and Urban, {Constantin F.} and Duncan Wilson",
note = "We would like to thank all members of the Aberdeen Fungal Group and the Department of Microbial Pathogenicity Mechanisms for ever fruitful conversations. In particular Al Brown for insightful discussions and Donna MacCallum for assistance in statistical analysis of in vivo data. As well as the Microscopy and Histology and the Cytometry and In vivo Imaging Core Facilities at the University of Aberdeen. DW is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (102549/Z/13/Z), a Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology (097377/Z/11/Z), a Wellcome Trust ISSF seed corn grant (RG12723 14), and the MRC and University of Aberdeen (MR/N006364/1). BH is supported by the Deutsche Forschungsgemeinschaft DFG SFB/TR 124 FungiNet, project C1. LLM is supported by a Royal Society Dorothy Hodgkin Fellowship (DH150187). ERB is supported by a BBSRC AFL Fellowship (BB/M014525/1). CFU was supported by grants of the Swedish Research Council VR-M 2014-2281, {\AA}ke Wiberg Foundation M15-0108 and Kempe Foundation SMK1453.",
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T1 - Biphasic zinc compartmentalisation in a human fungal pathogen

AU - Crawford, Aaron C.

AU - Lehtovirta, Laura E.

AU - Alamir, Omran

AU - Niemiec, Maria J.

AU - Alawfi, Bader

AU - Alsarraf, Mohammad

AU - Skrahina, Volha

AU - Costa, Anna C. B. P.

AU - Anderson, Andrew

AU - Yellagunda, Sujan

AU - Ballou, Elizabeth

AU - Hube, Bernhard

AU - Urban, Constantin F.

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N1 - We would like to thank all members of the Aberdeen Fungal Group and the Department of Microbial Pathogenicity Mechanisms for ever fruitful conversations. In particular Al Brown for insightful discussions and Donna MacCallum for assistance in statistical analysis of in vivo data. As well as the Microscopy and Histology and the Cytometry and In vivo Imaging Core Facilities at the University of Aberdeen. DW is supported by a Sir Henry Dale Fellowship jointly funded by the Wellcome Trust and the Royal Society (102549/Z/13/Z), a Wellcome Trust Strategic Award for Medical Mycology and Fungal Immunology (097377/Z/11/Z), a Wellcome Trust ISSF seed corn grant (RG12723 14), and the MRC and University of Aberdeen (MR/N006364/1). BH is supported by the Deutsche Forschungsgemeinschaft DFG SFB/TR 124 FungiNet, project C1. LLM is supported by a Royal Society Dorothy Hodgkin Fellowship (DH150187). ERB is supported by a BBSRC AFL Fellowship (BB/M014525/1). CFU was supported by grants of the Swedish Research Council VR-M 2014-2281, Åke Wiberg Foundation M15-0108 and Kempe Foundation SMK1453.

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N2 - Nutritional immunity describes the host-driven manipulation of essential micronutrients, including iron, zinc and manganese. To withstand nutritional immunity and proliferate within their hosts, pathogenic microbes must express efficient micronutrient uptake and homeostatic systems. Here we have elucidated the pathway of cellularzinc assimilation in the major human fungal pathogen Candida albicans. Bioinformatics analysis identified nine putative zinc transporters: four cytoplasmic-import Zip proteins (Zrt1, Zrt2, Zrt3 and orf19.5428) and five cytoplasmic-export ZnT proteins (orf19.1536/Zrc1, orf19.3874, orf19.3769, orf19.3132 and orf19.52). Only Zrt1 and Zrt2 are predicted to localise to the plasma membrane and here we demonstrate that Zrt2 is essential for C. albicans zinc uptake and growth at acidic pH. In contrast, ZRT1 expression was found to be highly pH-dependent and could support growth of the ZRT2-null strain at pH 7 and above. This regulatory paradigm is analogous to the distantly related pathogenic mould, Aspergillus fumigatus, suggesting that pH-adaptation of zinc transport may be conserved in fungi and we propose that environmental pH has shaped the evolution of zinc import systems in fungi. Deletion of C. albicans ZRT2 reduced fungal burden in wild type, but not in mice lacking the zinc-chelating antimicrobial protein calprotectin. Inhibition of zrt2 growth by neutrophil extracellular traps was calprotectin-dependent. This suggests that, within the kidney, C. albicans growth is determined by pathogen-Zrt2 and host-calprotectin. As well as serving as an essential micronutrient, zinc can also be highly toxic and we show that C. albicans deals with this potential threat by rapidly compartmentalising zinc within vesicular stores called zincosomes. In order to understand mechanistically how this process occurs, we created deletion mutants of all five ZnT-type transporters in C. albicans. Here we show that, unlike in Saccharomyces cerevisiae, C. albicans Zrc1 mediates zinc tolerance via zincosomal zinc compartmentalisation. This novel transporter was also essential for virulence and liver colonisation in vivo. In summary, we show that zinc homeostasis in a major human fungal pathogen is a multi-stage process initiated by Zrt1/Zrt2-cellular import, followed by Zrc1-dependent intracellular compartmentalisation.

AB - Nutritional immunity describes the host-driven manipulation of essential micronutrients, including iron, zinc and manganese. To withstand nutritional immunity and proliferate within their hosts, pathogenic microbes must express efficient micronutrient uptake and homeostatic systems. Here we have elucidated the pathway of cellularzinc assimilation in the major human fungal pathogen Candida albicans. Bioinformatics analysis identified nine putative zinc transporters: four cytoplasmic-import Zip proteins (Zrt1, Zrt2, Zrt3 and orf19.5428) and five cytoplasmic-export ZnT proteins (orf19.1536/Zrc1, orf19.3874, orf19.3769, orf19.3132 and orf19.52). Only Zrt1 and Zrt2 are predicted to localise to the plasma membrane and here we demonstrate that Zrt2 is essential for C. albicans zinc uptake and growth at acidic pH. In contrast, ZRT1 expression was found to be highly pH-dependent and could support growth of the ZRT2-null strain at pH 7 and above. This regulatory paradigm is analogous to the distantly related pathogenic mould, Aspergillus fumigatus, suggesting that pH-adaptation of zinc transport may be conserved in fungi and we propose that environmental pH has shaped the evolution of zinc import systems in fungi. Deletion of C. albicans ZRT2 reduced fungal burden in wild type, but not in mice lacking the zinc-chelating antimicrobial protein calprotectin. Inhibition of zrt2 growth by neutrophil extracellular traps was calprotectin-dependent. This suggests that, within the kidney, C. albicans growth is determined by pathogen-Zrt2 and host-calprotectin. As well as serving as an essential micronutrient, zinc can also be highly toxic and we show that C. albicans deals with this potential threat by rapidly compartmentalising zinc within vesicular stores called zincosomes. In order to understand mechanistically how this process occurs, we created deletion mutants of all five ZnT-type transporters in C. albicans. Here we show that, unlike in Saccharomyces cerevisiae, C. albicans Zrc1 mediates zinc tolerance via zincosomal zinc compartmentalisation. This novel transporter was also essential for virulence and liver colonisation in vivo. In summary, we show that zinc homeostasis in a major human fungal pathogen is a multi-stage process initiated by Zrt1/Zrt2-cellular import, followed by Zrc1-dependent intracellular compartmentalisation.

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