TY - JOUR
T1 - Birth after human chorionic gonadotropin-primed oocyte in vitro maturation and fertilization with testicular sperm in a normo-ovulatory patient
AU - González-Ortega, Claudia
AU - Pina Aguilar, Raul Eduardo
AU - Cancino-Villareal, Patricia
AU - Gutiérrez-Gutiérrez, Antonio Martin
N1 - REPA is supported by CONACYT (329705) and Elphinstone scholarships and a fellowship from IBSA Foundation.
PY - 2016/11/3
Y1 - 2016/11/3
N2 - In this report, we present a case of in vitro maturation (IVM) with surgical retrieved testicular sperm in a normo-ovulatory female. Human chorionic gonadotropin-primed IVM, testicular biopsy for sperm retrieval and intracytoplasmic sperm injection with fresh sperm were performed. Fourteen cumulus-oocyte complexes were obtained in germinal vesicle or metaphase I stage, eight oocytes reached metaphase II, seven presumptive zygotes were obtained, and three cleavage stages embryos in day 2 were transferred producing a singleton pregnancy. A single healthy newborn was obtained. Our results suggest that IVM may be an alternative for in vitro fertilization in normo-ovulatory women even if surgical retrieval of sperm is needed. Further research is required to depict contributing factors to the success of IVM in indications different from polycystic ovaries syndrome and the role of male gamete.
AB - In this report, we present a case of in vitro maturation (IVM) with surgical retrieved testicular sperm in a normo-ovulatory female. Human chorionic gonadotropin-primed IVM, testicular biopsy for sperm retrieval and intracytoplasmic sperm injection with fresh sperm were performed. Fourteen cumulus-oocyte complexes were obtained in germinal vesicle or metaphase I stage, eight oocytes reached metaphase II, seven presumptive zygotes were obtained, and three cleavage stages embryos in day 2 were transferred producing a singleton pregnancy. A single healthy newborn was obtained. Our results suggest that IVM may be an alternative for in vitro fertilization in normo-ovulatory women even if surgical retrieval of sperm is needed. Further research is required to depict contributing factors to the success of IVM in indications different from polycystic ovaries syndrome and the role of male gamete.
KW - Azoospermia
KW - germinal vesicle
KW - in vitro maturation
KW - intracytoplasmic sperm injection
KW - mild approach
KW - surgical retrieval of sperm
UR - http://www.scopus.com/inward/record.url?scp=84993296449&partnerID=8YFLogxK
U2 - 10.4103/0974-1208.192076
DO - 10.4103/0974-1208.192076
M3 - Article
C2 - 27803591
VL - 9
SP - 210
EP - 212
JO - Journal of Human Reproductive Sciences
JF - Journal of Human Reproductive Sciences
SN - 0974-1208
IS - 3
ER -
