Board-invited review: recent advances in biohydrogenation of unsaturated fatty acids within the rumen microbial ecosystem

T. C. Jenkins, R. J. Wallace, P. J. Moate, E. E. Mosley

Research output: Contribution to journalLiterature review

415 Citations (Scopus)

Abstract

Recent advances in chromatographic identification of CLA isomers, combined with interest in their possible properties in promoting human health (e. g., cancer prevention, decreased atherosclerosis, improved immune response) and animal performance (e. g., body composition, regulation of milk fat synthesis, milk production), has renewed interest in biohydrogenation and its regulation in the rumen. Conventional pathways of biohydrogenation traditionally ignored minor fatty acid intermediates, which led to the persistence of oversimplified pathways over the decades. Recent work is now being directed toward accounting for all possible trans-18:1 and CLA products formed, including the discovery of novel bioactive intermediates. Modern microbial genetics and molecular phylogenetic techniques for identifying and classifying microorganisms by their small-subunit rRNA gene sequences have advanced knowledge of the role and contribution of specific microbial species in the process of biohydrogenation. With new insights into the pathways of biohydrogenation now available, several attempts have been made at modeling the pathway to predict ruminal flows of unsaturated fatty acids and biohydrogenation intermediates across a range of ruminal conditions. After a brief historical account of major past accomplishments documenting biohydrogenation, this review summarizes recent advances in 4 major areas of biohydrogenation: the microorganisms involved, identification of intermediates, the biochemistry of key enzymes, and the development and testing of mathematical models to predict biohydrogenation outcomes.

Original languageEnglish
Pages (from-to)397-412
Number of pages16
JournalJournal of Animal Science
Volume86
Issue number2
Early online date27 Nov 2007
DOIs
Publication statusPublished - Feb 2008

Keywords

  • biohydrogenation
  • rumen
  • microorganism
  • conjugated linoleic acid
  • modeling
  • conjugated linoleic-acid
  • fungus neocallimastix frontalis
  • ribosomal-RNA sequences
  • lactating dairy-cows
  • butyrivibrio-fibrisolvens
  • oleic-acid
  • in-vitro
  • stearic-acid
  • sheep rumen
  • ruminal biohydrogenation
  • biohydrogenation
  • conjugated linoleic acid

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