TY - JOUR
T1 - Bound galloylated compounds in persimmon upcycled dietary fiber modulate microbial strains associated to human health after in vitro digestion
AU - Moreno-Chamba, Bryan
AU - Salazar-Bermeo, Julio
AU - Martínez-Madrid, María Concepción
AU - Lizama, Victoria
AU - Martín-Bermudo, Francisco
AU - Berná, Genoveva
AU - Neacsu, Madalina
AU - Saura, Domingo
AU - Martí, Nuria
AU - Valero, Manuel
N1 - Funding Information:
This research was financed by Ministry of Science Innovation and Universities through the funded project ‘Industrial symbiosis in the integral use of persimmon (Diospyros kaki); Bioeconomy example '(CTM2017-88978-R). The authors gratefully acknowledge Miss Laura Agulló and Mitra Sol Technologies S.L. for the given technical assistance.
PY - 2022/2/15
Y1 - 2022/2/15
N2 - Persimmon byproduct upcycling was performed by solvent-assisted extraction (SAE) to obtain dietary fiber (DF). The effect of SAE on DF modulation was studied on specific beneficial and pathogenic strains before and after the in vitro digestion process. Overall, digested DF samples extracted using acetone as a solvent (dCET) showed higher (p < 0.05) prebiotic activity scores (PASs) in beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus casei, Lactococcus lactis, and Streptococcus salivarius. Moreover, dCET reduced the tested pathogenic strain populations. Initial cell attachment (ICA) inhibitory activity on biofilm formation by Pseudomonas putida, Staphylococcus aureus and Bacillus subitilis was observed for dCET, as well as inhibition of preformed (PFB) S. aureus biofilms. dCET combined with the antibiotics kanamycin (K) or gentamycin (G) exhibited synergistic effects against all tested pathogens, displaying bactericidal effects against S. aureus. High-performance liquid chromatography (HPLC-DAD) analysis showed that after hydrolysis, the released gallic acid could have been responsible for the antimicrobial properties registered in DF from the persimmon byproduct. The obtained results provided information about the potential of upcycled persimmon fiber fractions as possible prebiotics, although further research must be performed with complex microbial populations and in vivo studies.
AB - Persimmon byproduct upcycling was performed by solvent-assisted extraction (SAE) to obtain dietary fiber (DF). The effect of SAE on DF modulation was studied on specific beneficial and pathogenic strains before and after the in vitro digestion process. Overall, digested DF samples extracted using acetone as a solvent (dCET) showed higher (p < 0.05) prebiotic activity scores (PASs) in beneficial bacteria such as Bifidobacterium bifidum, Lactobacillus casei, Lactococcus lactis, and Streptococcus salivarius. Moreover, dCET reduced the tested pathogenic strain populations. Initial cell attachment (ICA) inhibitory activity on biofilm formation by Pseudomonas putida, Staphylococcus aureus and Bacillus subitilis was observed for dCET, as well as inhibition of preformed (PFB) S. aureus biofilms. dCET combined with the antibiotics kanamycin (K) or gentamycin (G) exhibited synergistic effects against all tested pathogens, displaying bactericidal effects against S. aureus. High-performance liquid chromatography (HPLC-DAD) analysis showed that after hydrolysis, the released gallic acid could have been responsible for the antimicrobial properties registered in DF from the persimmon byproduct. The obtained results provided information about the potential of upcycled persimmon fiber fractions as possible prebiotics, although further research must be performed with complex microbial populations and in vivo studies.
KW - Antibacterial synergy testing
KW - Antimicrobial activity
KW - Dietary polysaccharides
KW - Persimmon byproducts
KW - Prebiotic activity
UR - http://www.scopus.com/inward/record.url?scp=85121848928&partnerID=8YFLogxK
U2 - 10.1016/j.lwt.2021.113011
DO - 10.1016/j.lwt.2021.113011
M3 - Article
AN - SCOPUS:85121848928
VL - 156
JO - LWT- Food Science and Technology
JF - LWT- Food Science and Technology
SN - 0023-6438
M1 - 113011
ER -
